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hisF from Brucella melitensis bv. 1 str. 16M

Victor ID 1325
Gene Name hisF from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1197752
NCBI Protein GI 17988324
Locus Tag BMEI2041
Protein Accession NP_540958.1
Other Database IDs UniProtKB-ID: D0B3X4_BRUME
UniRef100: UniRef100_C0RFX3
UniRef90: UniRef90_C0RFX3
UniRef50: UniRef50_Q2KTT1
UniParc: UPI00000582DC
EMBL: GG703778
EMBL-CDS: EEW88098.1
RefSeq_NT: NC_003317.1
OMA: RVVKGTN
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 2102198
Gene Ending Position 2102983
Gene Strand (Orientation) -
Protein Name imidazole glycerol phosphate synthase subunit HisF
DNA Sequence 
>gi|17986284:2102198-2102983 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
ATCAACGGACCGGATCGAGCCGCATCGGAATGCCGGCTTCGGCCATATAGCGCTTGGCCTCGCCGATCGT
ATAGGTGCCGAAGTGGAAAATCGAAGCGGCGAGAACCGCCGTCGCATGGCCATCGCGAATGCCCGCCACC
AGATGATCGAGAGTGCCGACGCCGCCCGATGCGATGACCGGCGCGCGCACGCTATCGGCCACGGCGCGGG
TCAGCGCCACGTCGTACCCCGCCTTGGTGCCATCGCGGTCCATGGAGGTGAGCAGGATTTCGCCTGCGCC
AAGGTCCACGACCTTCTGTGCAAATTCCACCGCATCGATGCCGGTCGGCTGCCGCCCGCCATGGGTGAAG
ATTTCCCAGTGATCATTTTCACCCGCGCCGGAAACCTTTTTCGCATCGATGGCGACAACAATGCATTGAT
TGCCAAACTTGTCGGCGGCTTCCGCCACGAATTCCGGGTTCTTCACCGCTGCGGTATTGATCGACACCTT
GTCGGCTCCCGCCAAAAGAAGCTTGCGGATATCGGCCACCTGGCGAACCCCGCCACCAACGGTCAAGGGC
ATGAAGCACTGTTCGGCTGTACGCGCCACCACATCGAAAATTGTCTCGCGATTGTCCGAAGACGCCGTGA
TGTCGAGAAAGCACAATTCGTCGGCGCCTGCCGCATCATAGGCGCGGGCGGCTTCCACCGGATCACCGGC
ATCGATCAGATCAACGAAGTTCACACCTTTGACGACGCGGCCATCTTTCACATCGAGACAGGGAATCACT
CTTGCTTTCAAAGTCA
Protein Sequence 
>gi|17988324|ref|NP_540958.1| imidazole glycerol phosphate synthase subunit HisF [Brucella melitensis bv. 1 str. 16M] MTLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAARAYDAAGADELCFLDITASSDNRETIFDVVARTAEQCFMPLTVGGGVRQVADIRKLLLAGADKVSINTAAVKNPEFVAEAADKFGNQCIVVAIDAKKVSGAGENDHWEIFTHGGRQPTGIDAVEFAQKVVDLGAGEILLTSMDRDGTKAGYDVALTRAVADSVRAPVIASGGVGTLDHLVAGIRDGHATAVLAASIFHFGTYTIGEAKRYMAEAGIPMRLDPVR
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit (By similarity)(UniProt: Q8FY07).

CATALYTIC ACTIVITY: 5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H(2)O(UniProt: Q8FY07).

PATHWAY: Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5(UniProt: Q8FY07).

SUBUNIT: Heterodimer of hisH and hisF (By similarity)(UniProt: Q8FY07).

SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: Q8FY07).

SIMILARITY: Belongs to the hisA/hisF family(UniProt: Q8FY07).

MUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis hisF gene is required for intracellular multiplication in human macrophage THP-1 cells (Kohler et al., 2002).
COG COG0107E, under E: Amino acid transport and metabolism
References
Kohler et al., 2002: Kohler S, Foulongne V, Ouahrani-Bettache S, Bourg G, Teyssier J, Ramuz M, Liautard JP. The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. Proceedings of the National Academy of Sciences of the United States of America. 2002 Nov 26; 99(24); 15711-6. [PubMed: 12438693 ].