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dnaK from Brucella melitensis bv. 1 str. 16M |
| Victor ID |
1322 |
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Gene Name |
dnaK from Brucella melitensis bv. 1 str. 16M |
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Sequence Strain (Species/Organism) |
Brucella melitensis bv. 1 str. 16M |
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NCBI Gene ID |
1197713
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NCBI Protein GI |
17988285
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Locus Tag |
BMEI2002 |
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Protein Accession |
NP_540919.1 |
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Other Database IDs |
UniProtKB-ID: DNAK_BRUME
UniRef100: UniRef100_Q8YE76
UniRef90: UniRef90_Q05981
UniRef50: UniRef50_Q8DH10
UniParc: UPI00001295B3
EMBL: AE008917
EMBL-CDS: AAL53183.1
RefSeq_NT: NC_003317.1
GenomeReviews: AE008917_GR
KEGG: bme:BMEI2002
NMPDR: fig|224914.1.peg.2001
HOGENOM: HBG334976
OMA: TKFFGKE
ProtClustDB: PRK00290
BioCyc: BMEL224914:BMEI2002-MONOMER
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Taxonomy ID |
224914
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Chromosome No |
I |
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Gene Starting Position |
2059669 |
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Gene Ending Position |
2061594 |
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Gene Strand (Orientation) |
- |
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Protein Name |
molecular chaperone DnaK |
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DNA Sequence |
>gi|17986284:2059669-2061594 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
ATTACGACGACTTCTTGTTGTCGTCGATTTCTTCGTAGTCGGCATCGACCACATCGTCCTTGGACGAAGA
GGCCTGTTCACCGCCCTCGGCACCCGCACCTTCGGCAGCCTGCGCTGCTTCGTACATGGCCTGACCAAGC
TTCATGGAAACTTCGGCAAGGGCCTGGGTCTTCGCCTTGATATCCTCGGCGTCTTCACCTTCAAGCGAGG
TCTTGAGGGCTGCGATCGCATCCTCAATGGCCTTCTTGTCATCGGCCGAAACCTTGTCGCCATATTCGGC
CAGCGACTTTTCGGTCGAATGGACCAGGCTTTCGGCCTGATTCTTGGCTTCGACGCTCTCACGACGCTTC
TTGTCGGCCTCGGCATTGGCTTCGGCGTCCTTGACCATCTTTTCGATGTCGGCATCGCTCAGGCCGCCCG
AAGCCTGAATGCGGATCTGGTGCTCCTTGCCGGTACCCTTATCCTTGGCCGAAACGTTGACGATGCCGTT
GGCGTCGATGTCGAAGGTCACTTCGATCTGCGGCACACCGCGCGGTGCGGGCGGAATGCCAACGAGGTCG
AACTGTCCAAGCAGCTTGTTATCGGCTGCCATTTCACGCTCGCCCTGGAAGACGCGGATCGTCACGGCCG
ACTGGTTGTCCTCAGCCGTGGAGAAGGTCTGCGACTTCTTGGTCGGGATAGTGGTGTTGCGTTCGATCAG
GCGGGTGAACACGCCGCCCAGCGTTTCAATGCCGAGCGAAAGCGGGGTCACGTCGAGCAGCAGCACGTCC
TTGACGTCGCCCTGCAAAACGCCGCCCTGGATCGCCGCGCCCATGGCCACGACTTCATCCGGGTTCACGC
CCTTGTGCGGTTCCTTGCCGAAGAAGGCCTTCACGACTTCCTGAATCTTGGGCATGCGGGTCATGCCGCC
GACCAGAACCACTTCGTCAATTTCGCCAGCCTTGAGGCCGGCATCCTTGAGCGCCGCCTTGCACGGCTCG
ACCGTGCGCTGCACGAGATCATCGACCAGGCTTTCAAACTTGGCGCGCGACAGCTTGATCGCCAGATGCT
TCGGGCCAGTCTGGTCAGCCGTGATGAACGGCAGGTTGATTTCGGTCTGCTGCGAGGACGACAGTTCGAT
CTTGGCCTTTTCGGCAGCTTCCTTGAGGCGCTGCAGGGCAAGCTTGTCGTTCTTCAGGTCGATGCCACTT
TCCTTCTTGAACTCGGCAACCAGATATTCGACCAGACGAATATCGAAGTCTTCACCGCCAAGGAACGTGT
CGCCATTGGTGGACTTCACTTCAAAAACGCCGTCGCCGATTTCCAGAACCGACACGTCGAAAGTACCGCC
GCCAAGGTCATAGACAGCGATGGTCTTGCCTTCGCTCTTGTCGAGACCATAGGCAAGCGCAGCCGCGGTC
GGCTCGTTGATGATGCGCAGAACTTCCAGACCGGCGATCTTGCCGGCATCCTTGGTGGCCTGACGCTGGG
CGTCGTTGAAGTAGGCCGGAACCGTGATGACGGCCTGCGTAACGGTTTCGCCAAGGTAGGATTCAGCCGT
TTCCTTCATCTTCTGAAGGATCATGGCGGAGATCTGCGACGGAGAATATTTCTTGCCGTGCACCTCGACC
CAGGCATCGCCATTGTCGCCCTTGACGATCTTGTAAGGAACAAGATCCTTGTCCTTGGTGACCATCGGAT
CGTCATAGCGACGGCCAATCAGGCGCTTGACGGCAAAAAGGGTGCCTTCCGGGTTGGTGACGGCCTGACG
CTTCGCAGGCTGACCAGCGAGGCGTTCGTCACCATCGGTGAAGGCGATGATCGAAGGGGTGGTGCGTGCA
CCTTCTGCATTTTCAATGACCTTCGCGTTCTTTCCGTCCATGACGGCAACGCAGGAGTTGGTCGTTCCCA
TATCGATACCAATAACTTTAGCCATATTTCTCTCCA
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Protein Sequence |
>gi|17988285|ref|NP_540919.1| molecular chaperone DnaK [Brucella melitensis bv. 1 str. 16M] MERNMAKVIGIDMGTTNSCVAVMDGKNAKVIENAEGARTTPSIIAFTDGDERLAGQPAKRQAVTNPEGTLFAVKRLIGRRYDDPMVTKDKDLVPYKIVKGDNGDAWVEVHGKKYSPSQISAMILQKMKETAESYLGETVTQAVITVPAYFNDAQRQATKDAGKIAGLEVLRIINEPTAAALAYGLDKSEGKTIAVYDLGGGTFDVSVLEIGDGVFEVKSTNGDTFLGGEDFDIRLVEYLVAEFKKESGIDLKNDKLALQRLKEAAEKAKIELSSSQQTEINLPFITADQTGPKHLAIKLSRAKFESLVDDLVQRTVEPCKAALKDAGLKAGEIDEVVLVGGMTRMPKIQEVVKAFFGKEPHKGVNPDEVVAMGAAIQGGVLQGDVKDVLLLDVTPLSLGIETLGGVFTRLIERNTTIPTKKSQTFSTAEDNQSAVTIRVFQGEREMAADNKLLGQFDLVGIPPAPRGVPQIEVTFDIDANGIVNVSAKDKGTGKEHQIRIQASGGLSDADIEKMVKDAEANAEADKKRRESVEAKNQAESLVHSTEKSLAEYGDKVSADDKKAIEDAIAALKTSLEGEDAEDIKAKTQALAEVSMKLGQAMYEAAQAAEGAGAEGGEQASSSKDDVVDADYEEIDDNKKSS
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Molecule Role |
Virulence factor |
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Molecule Role Annotation |
FUNCTION: Acts as a chaperone (By similarity)(UniProt: Q8FXX2).
INDUCTION: By stress conditions e.g. heat shock (By similarity)(UniProt: Q8FXX2).
SIMILARITY: Belongs to the heat shock protein 70 family(UniProt: Q8FXX2).
MUTATION: The heat shock protein DnaK is essential for intramacrophagic replication of Brucella suis. The replacement of the stress-inducible, native dnaK promoter of B suis by the promoter of the constitutively expressed bla gene resulted in temperature-independent synthesis of DnaK. In contrast to a dnaK null mutant, this strain grew at 37 degrees C, with a thermal cutoff at 39 degrees C However, the constitutive dnaK mutant, which showed high sensitivity to H(2)O(2)-mediated stress , failed to multiply in murine macrophage-like cells and was rapidly eliminated in a mouse model of infection, adding strong arguments that stress-mediated and heat shock promoter-dependent induction of dnaK is a crucial event in the intracellular replication of B suis (Köhler et al., 2002).
Mutation studies indicated that DnaK, but not DnaJ, was required for growth at 37 degrees C in vitro. Viability of the dnaK null mutant was also greatly affected at low pH. In infection experiments performed with both mutants at the reduced temperature of 30 degrees C, the dnaK mutant of B suis survived but failed to multiply within U937 cells, whereas the wild-type strain and the dnaJ mutant multiplied normally. Complementation of the dnaK mutant with the cloned dnaK gene restored growth at 37 degrees C, increased resistance to acid pH, and increased intracellular multiplication (Köhler et al., 2002). |
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COG
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COG0443O, under O: Posttranslational modification, protein turnover, chaperones |
| References |
Köhler et al., 2002: Köhler S, Ekaza E, Paquet JY, Walravens K, Teyssier J, Godfroid J, Liautard JP. Induction of dnaK through its native heat shock promoter is necessary for intramacrophagic replication of Brucella suis. Infection and immunity. 2002; 70(3); 1631-1634. [PubMed: 11854256].
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