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mutM from Brucella melitensis bv. 1 str. 16M

Victor ID 1321
Gene Name mutM from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1197657
NCBI Protein GI 17988229
Locus Tag BMEI1946
Protein Accession NP_540863.1
Other Database IDs UniProtKB-ID: D0B465_BRUME
UniRef100: UniRef100_B2S9T2
UniRef90: UniRef90_B2S9T2
UniRef50: UniRef50_Q13EN7
UniParc: UPI000005827D
EMBL: GG703778
EMBL-CDS: EEW88189.1
RefSeq_NT: NC_003317.1
OMA: PDLRFPF
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 2005059
Gene Ending Position 2005940
Gene Strand (Orientation) -
Protein Name formamidopyrimidine-DNA glycosylase
DNA Sequence 
>gi|17986284:2005059-2005940 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
CTCATGTCTGGCAAGAGGCACAGAAAAAAGTCGAACGACCAGATTGCACCACGCGCTCGACAGTGCCGCC
GCAGGCGGGGTTTCTGCATGGCTTACCCTCGCGGCCATAGACGGAAAAAGAGTGCTGGAAATAGCCGAGC
GCACCGTCGGCCTGAATGTAATCCTTAAGGCTGGAGCCTCCGGCTGCAATCGCCTGCGCAATGACGGAGC
GAATGGCCCCGGCAAGCCGCTCCATCACGTCCATTTCGCCTGCAACCGAGCCTGCCGCGCGCATCGGCGA
AAGCCCTGGGCGCCAGAGCGCCTCGCAGACATAGATATTGCCAAGCCCCGCGATAAGCCTCTGGTCGAGC
AAGGCGGCCTTCAGGGGCGTGCGGCGTCCCTTGAAAAGCGCGGCCAGAACCTCGCCCGACAGGAGATTGC
CGGTCGGCTCCACACCAAGGTCTTTCAGGAGCGGATGTTCCTCCAGCGCGCCCTTTTCGGCAAAGAGCAT
GAAACCGAAGCGGCGCGGATCATTATAAATAATGCGTGCAGACGCACCGTCCGGGCGCATGAGGTGAAAG
ACCACATGGTCATGCAGGCTGTTCTTGGAGCGCTCGTGATGAAACCCGCCCGGCAAACCTTGCGCATCCT
CCGCCTCAATGCGAAACGAGCCGGACATGCCGAGATGGCTGATGATCGAAAGCCCGTCATCGAGATGGAC
GGTCAGATATTTCGCACGGCGGCCAAGCGCCTCGATGCGCCTGCCGGAAAGGCGTTCGGCAAAATTTTCA
GGAAAGGCAAAGCGCAGGTCGGGCCGATTCTGCTCCACCCGCACAACGGTTGCCCCTTCCATGAAGGGTT
GCAGACCACGGCGAACCGTTTCGACCTCTGGCAGTTCCGGCA
Protein Sequence 
>gi|17988229|ref|NP_540863.1| formamidopyrimidine-DNA glycosylase [Brucella melitensis bv. 1 str. 16M] MPELPEVETVRRGLQPFMEGATVVRVEQNRPDLRFAFPENFAERLSGRRIEALGRRAKYLTVHLDDGLSIISHLGMSGSFRIEAEDAQGLPGGFHHERSKNSLHDHVVFHLMRPDGASARIIYNDPRRFGFMLFAEKGALEEHPLLKDLGVEPTGNLLSGEVLAALFKGRRTPLKAALLDQRLIAGLGNIYVCEALWRPGLSPMRAAGSVAGEMDVMERLAGAIRSVIAQAIAAGGSSLKDYIQADGALGYFQHSFSVYGREGKPCRNPACGGTVERVVQSGRSTFFCASCQT
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity)(UniProt: Q8FXR6).

CATALYTIC ACTIVITY: Hydrolysis of DNA containing ring-opened N(7)-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine(UniProt: Q8FXR6).

CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate(UniProt: Q8FXR6).

COFACTOR: Binds 1 zinc ion per subunit (By similarity)(UniProt: Q8FXR6).

SUBUNIT: Monomer (By similarity)(UniProt: Q8FXR6).

SIMILARITY: Belongs to the FPG family(UniProt: Q8FXR6).

SIMILARITY: Contains 1 FPG-type zinc finger(UniProt: Q8FXR6).

MUTATION: Attenuated in Differential fluorescence induction (Delrue et al., 2004).
COG COG0266L, under L: Replication, recombination and repair
References
Delrue et al., 2004: Delrue RM, Lestrate P, Tibor A, Letesson JJ, De Bolle X. Brucella pathogenesis, genes identified from random large-scale screens. FEMS microbiology letters. 2004; 231(1); 1-12. [PubMed: 14979322].