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ilvD from Brucella melitensis bv. 1 str. 16M

Victor ID 1313
Gene Name ilvD from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1197559
NCBI Protein GI 17988131
Locus Tag BMEI1848
Protein Accession NP_540765.1
Other Database IDs UniProtKB-ID: ILVD_BRUME
UniRef100: UniRef100_Q8YEN0
UniRef90: UniRef90_B2S7Z5
UniRef50: UniRef50_Q5F8G6
UniParc: UPI000012D56A
EMBL: AE008917
EMBL-CDS: AAL53029.1
RefSeq_NT: NC_003317.1
GenomeReviews: AE008917_GR
KEGG: bme:BMEI1848
NMPDR: fig|224914.1.peg.1847
HOGENOM: HBG671001
OMA: KVPCLSK
ProtClustDB: PRK12448
BioCyc: BMEL224914:BMEI1848-MONOMER
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 1897913
Gene Ending Position 1899754
Gene Strand (Orientation) +
Protein Name dihydroxy-acid dehydratase
DNA Sequence 
>gi|17986284:1897913-1899754 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
TTTGAAAATGCCTCCTTATCGTTCGCGTACAACCACCCATGGCCGCAATATGGCCGGCGCGCGCGGCCTA
TGGCGCGCCACCGGCATGAAGGATGAGGATTTCGGCAAGCCGATTATCGCCGTGGTGAACTCGTTCACCC
AGTTCGTGCCCGGCCATGTGCATCTGAAGGATCTCGGCCAGCTCGTCGCGCGCGAAATCGAAAGTGCGGG
CGGCGTCGCCAAGGAATTCAATACCATTGCGGTCGATGACGGCATCGCCATGGGCCATGACGGCATGCTC
TATTCGCTCCCCTCCCGTGAGCTCATCGCCGACTCGGTCGAATATATGGTCAATGCGCATTGCGCCGATG
CGATGGTCTGCATCTCCAATTGCGACAAGATCACGCCCGGCATGCTGATGGCGGCGCTGCGTCTCAATAT
TCCGGTCGTGTTCGTCTCCGGTGGGCCGATGGAAGCAGGCAAGGTCGTGTGGGAGGATTCTGTCAAGAAG
CTCGACCTCGTGGACGCCATGGTGGCGGCGGCCGACGACCATTATACCGACGAGCAGGTGAAGGCGATTG
AGCGTTCCGCCTGCCCGACCTGTGGGTCGTGTTCGGGCATGTTCACGGCCAATTCGATGAATTGCCTTAC
CGAAGCGCTCGGCCTGTCGCTGCCCGGCAATGGTTCGACGCTCGCCACCCATGCAGACCGCAAGCGCCTC
TTCGTCGAAGCTGGCCACCTGATCGTCGATCTCGCCCGCCGCTATTACGAGCAGGATGACGAAAGTGTGC
TGCCGCGCTCCATCGCCACTTTCTCGGCTTTCGAGAACGCGATGACGCTCGATATCGCCATGGGCGGTTC
GACCAATACGGTTCTGCATCTGCTCGCGGCTGCGCAGGAAGCGGAAATCGATTTCACCATGGCGGATATA
GACCGTCTGTCGCGCCGCGTGCCGGTGCTGTGCAAGGTGGCGCCCGCCGTGTCGTCGGTTCACATGGAAG
ACGTGCATCATGCCGGCGGCATCATGGGCATTCTCGGACAGCTCGACAATGCGGGCCTGCTGACCACCAG
CATCCCGACCGTGCACAGCGAAACGCTCGCTAAGGCGCTTGATCATTGGGATGTAACGCGCACAAACAGC
GAAATGGTGCACAAATTCTATTCCGCCGCTCCCGGCGGCGTGCCGACGCAGGTGGCTTTCAGCCAGGAGC
GGCGCTTCGACAAGGTGGACACCGACCGCGAAAAAGGCGTCATCCACTCAAAGGAACACGCTTTCAGCCA
GGATGGCGGCTTGGCCGTGCTTTACGGCAATCTGGCGGAAGACGGCTGCATCGTGAAGACGGCAGGCGTG
GACGATTCCATCCTGAAATTCTCCGGCCCGGCCCGCATTTTTGAAAGCCAGGATTCGGCTGTGCTTGGCA
TTTTGAACGGCAAGATCAAGCCGGGCGACATCGTGCTGATCCGCTATGAAGGCCCGCGCGGCGGCCCCGG
AATGCAGGAAATGCTCTACCCGACCAGCTATCTGAAGTCGAAGGGCCTCGGCAAAGCCTGCGCACTTATC
ACCGACGGACGTTTCTCGGGCGGTTCATCGGGCCTTTCCATCGGCCATGTTTCGCCGGAAGCAGCCGAAG
GCGGCACGATCGGTCTGGTGCGCGAAGGCGATATCATCGATATCGACATTCCGAACCGCAAGATCCACCT
TGCCGTTGACGACGCCACACTTGCCGAACGCCGCGCCGAGCAGGATGCAGCAGGCTGGAAACCGGCAGAG
GAACGCAAGCGTAAAATCTCCACTGCGCTGAAAGCCTATGCGGCAATGGCGACAAGTGCCGCCAGGGGCG
CGGTGCGAAAGCTGCCGGATTG
Protein Sequence 
>gi|17988131|ref|NP_540765.1| dihydroxy-acid dehydratase [Brucella melitensis bv. 1 str. 16M] MKMPPYRSRTTTHGRNMAGARGLWRATGMKDEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIESAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAALRLNIPVVFVSGGPMEAGKVVWEDSVKKLDLVDAMVAAADDHYTDEQVKAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHADRKRLFVEAGHLIVDLARRYYEQDDESVLPRSIATFSAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMADIDRLSRRVPVLCKVAPAVSSVHMEDVHHAGGIMGILGQLDNAGLLTTSIPTVHSETLAKALDHWDVTRTNSEMVHKFYSAAPGGVPTQVAFSQERRFDKVDTDREKGVIHSKEHAFSQDGGLAVLYGNLAEDGCIVKTAGVDDSILKFSGPARIFESQDSAVLGILNGKIKPGDIVLIRYEGPRGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGSSGLSIGHVSPEAAEGGTIGLVREGDIIDIDIPNRKIHLAVDDATLAERRAEQDAAGWKPAEERKRKISTALKAYAAMATSAARGAVRKLPD
Molecule Role Virulence factor
Molecule Role Annotation CATALYTIC ACTIVITY: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H(2)O(UniProt: Q8G353).

COFACTOR: Binds 1 4Fe-4S cluster (Potential)(UniProt: Q8G353).

PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3(UniProt: Q8G353).

PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3(UniProt: Q8G353).

SIMILARITY: Belongs to the ilvD/edd family(UniProt: Q8G353).

MUTATION: Of those B abortus mutants with mini-Tn5 insertions in genes predicted to be involved in amino acid biosynthesis and transport, only the ilvD mutant, displayed attenuation in both macrophages and mice. The othre two amino acid biosynthesis mutants [trpB::miniTn5 and pheA::miniTn5] displayed wild-type virulence in mice but attenuated inside macrophages. The studies with B abortus ilvD, trpB, and pheA mutants suggest that tryptophan and phenylalanine are available to the brucellae in their intracellular niche but that other amino acids (eg, leucine, isoleucine, or valine) are not (Alcantara et al., 2004).
COG COG0129EG, under E: Amino acid transport and metabolism; G: Carbohydrate transport and metabolism
References
Alcantara et al., 2004: Alcantara RB, Read RD, Valderas MW, Brown TD, Roop RM 2nd. Intact purine biosynthesis pathways are required for wild-type virulence of Brucella abortus 2308 in the BALB/c mouse model. Infection and immunity. 2004; 72(8); 4911-4917. [PubMed: 15271960].