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glnD from Brucella melitensis bv. 1 str. 16M

Victor ID 1309
Gene Name glnD from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1197515
NCBI Protein GI 17988087
Locus Tag BMEI1804
Protein Accession NP_540721.1
Other Database IDs UniProtKB-ID: D0B4K1_BRUME
UniRef100: UniRef100_B2S8D8
UniRef90: UniRef90_B2S8D8
UniRef50: UniRef50_Q8UIF1
UniParc: UPI00000581F0
EMBL: GG703778
EMBL-CDS: EEW88325.1
RefSeq_NT: NC_003317.1
OMA: MQHDLFH
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 1842670
Gene Ending Position 1845474
Gene Strand (Orientation) +
Protein Name PII uridylyl-transferase
DNA Sequence 
>gi|17986284:1842670-1845474 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
CATGAGCGCACACGACCTGAAGCTGGAAGAGATCGTCAATGCCGAAACGCTCCGTCGCAAGCTCAATGAA
CTGGCGGACACGGCCGATGAAAGCTACACCAGCCTTCCCATGCGCAAGGTTGTGCTCCAGACGCTGAAGG
ATGCGCTGGCGAGCGGGCGCGCCAATGCCGAAGACATGCTGATGAAGGATGGCGGCGGCACGCTTTGCGC
CAAGCGGCTCTGCTACCTGATGGATACGCTGATCGACATCCTGTTTGAATTCGCAACCACCAGGGCCTAT
CCGACGCGCAATCCCTCCAAGGCGGAGAATATGGCGCTCGTGGCAGTCGGCGGCTATGGGCGCGGCGGGC
TTGCACAGGGTTCCGACATCGACCTTCTTTTCCTGCTTCCCTATAAACAGACGCCCTGGGGCGAACAGGT
TGTGGAATATACGCTCTACATGCTCTGGGACATGGGGCTGAAAGTCGGCCATTCCACCCGCAACATCGAT
GAGTGCATTCGTCTGGCGCGCGAGGACATGACCATTCGCACAGCCCTTCTCGATGCGCGCTTTCTTACCG
GCGACAAGGATCTGTTCCGCACACTTGAAATCCGTTTCGAGGAGGAAATCGTCAAGGGAACGGAGCCGGA
ATTCATTCAGGCAAAGCTTGCCGAGCGCGATGCACGCCACCGCAAGGCAGGTGAAACACGCTATCTGGTC
GAACCGAACGTCAAGGAAGGCAAGGGCGGACAGCGCGACCTGCACACCCTGTTCTGGATCACCAAATATT
TCTACCGCGTGAAGACCAAGGAAGAGCTGGTCAAGCTTGGGGTTCTGTCGCGAGCGGAACTGAAATTGTT
CAACAAGGCTGAGGATTTTCTCTGGGCGGTGCGGTGCCATATGCATTTTGCGACGCTGAAAGCCGAAGAA
CGCCTTTCCTTCGACATTCAGCCGGAAATCGCACAGCGCCTCGGCTATACGGCGCATCCCGGCCAGAACT
ATGTCGAACGCTTCATGAAGCATTATTTCCTCGTCGCGAAGGATGTGGGTGACCTCACCCGCATCATCTG
CGCGGCGCTGGAAGAACAGCAGGCAAAGCATGTTCCAGGCTTCAACCGCATCTTCCTGACCTTCTCGCGC
CGCAAGCGCAAGCTTTCGGATGATGGCGCTTTCATTTCGGAAAACCATCGCATCAATATCGCACGGCCCG
ATATTTTCCGGCAGGACCCGGTCAATATGATCCGGCTCTTTCACCTTGCCGACCGCCATGGGCTGGAGTT
TCATCCCGAAGCAATGCAGAGCCTCACCCGCTCGCTGAAGCTCATCAATGCCGACCTGCGCGAGAACCCG
GAGGCCAACCGGCTTTTCCTCGAAATCCTCACCTCGCCGCGCAATCCGGAACTTATCCTGCGCCGCATGA
ACGAATCCGGCGTGCTTGGAAAATTCATTCCCGATTTCGGCAAGATCGTCGCGATGATGCAGTTCAATAT
GTATCACCACTATACGGTGGACGAGCATCTGTTGCGCTGCATTGCCGTGCTTTCGGAAATCGAGCACGGC
GAGCTTAAAACCGAGCACCCGCTTTCAAACCATCTCATCACCACGATCAAGCGCGACCGCAACCTGCTTT
ACGTGACGCTTCTTCTGCACGATATCGCCAAGGGCCGCCCGGAAGACCACTCCATCGCGGGCGCACGCAT
TGCCCGCAGGCTTTGCCCGCGCTTCGGCTTGACACCATCCGAAACCGAGACCGTGGAATGGCTGGTGCGC
GAACACCTCACCATGAGCATGGTGGCGCAATCGCGCGATCTCAACGACCGCAAGACCATTATCGATTTTG
CCGATACGGTGCAGACGATGGAGCGGCTGAAGCTTCTCCTGATCCTCACCGTTTGTGATATCAAGGCGGT
CGGCCCCGGCATATGGAACGGCTGGAAAGGCCAGCTTCTGCGCACGCTTTTCTATGAGACCGAACTGGTG
CTGACGGGCGGATTTTCCGAATTGTCGCGGGCCGCCCGCGACAAGCAGGCGCGTGAGGCGCTGGCCGAGC
GGCTTTCCGACTGGCCGAAGGAAGAGCGCGACGCCTATCTTGCCCTGCCCTATACCAATTATTTCCTCAC
CGTCAGCCTGGACGATCAGGTGCGCCATGCGCATTTCATACGCGATGCCGACCAGCAGGGCCGTGCGCTC
GTCACCATGGCCAAGCCGCACGCATTCGAGGCCGTGACCGAAATTACCGTTCTCGCGCCCGACCATCCGC
GTCTCCTTTCGGTCATTACCGGAGCCTGCGCGGCAGCGGGCGGCAATATCGTGGATGCGCAGATTTTTAC
CACCAGCGACGGGCGTGCGCTGGACACGATCCTCATCAGCCGCGAATTCGACACCGACGACGACGAACGC
CGCCGGGCCGAGCGCGTGGGCAAGGTGATCGAGGACGTGCTTTCCGGCAAGGCGCATCTGCCCGATATGC
TGGCGAAGCGCACCAAGCCGAAGAAAGCTGCCAGGGCTTTCAAGGTGGAGCCGCGTGTCGAGATCAACAA
CACGCTTTCCAACAAATTCACGGTTATCGAAGTGGAAGGTCTGGACCGGCCGGGCCTGCTTTCAGAGCTG
ACCGGCCTTATTTCCGACCTTTCACTGGATATCGCTTCGGCCCATATCACCACTTTCGGCGAGAAGGTGA
TCGACAGTTTTTATGTGACGGATCTCGTGGGCCACAAGATTTCAAACGCCACCCGCCAGGGCAATATCAA
GCGCAAGCTCCTTGCCCTTCTGGGCGCAGAGAACGGCGCCAGGACAAACGGCCGCTCGCCACAGGCCGCA
GCATA
Protein Sequence 
>gi|17988087|ref|NP_540721.1| PII uridylyl-transferase [Brucella melitensis bv. 1 str. 16M] MSAHDLKLEEIVNAETLRRKLNELADTADESYTSLPMRKVVLQTLKDALASGRANAEDMLMKDGGGTLCAKRLCYLMDTLIDILFEFATTRAYPTRNPSKAENMALVAVGGYGRGGLAQGSDIDLLFLLPYKQTPWGEQVVEYTLYMLWDMGLKVGHSTRNIDECIRLAREDMTIRTALLDARFLTGDKDLFRTLEIRFEEEIVKGTEPEFIQAKLAERDARHRKAGETRYLVEPNVKEGKGGQRDLHTLFWITKYFYRVKTKEELVKLGVLSRAELKLFNKAEDFLWAVRCHMHFATLKAEERLSFDIQPEIAQRLGYTAHPGQNYVERFMKHYFLVAKDVGDLTRIICAALEEQQAKHVPGFNRIFLTFSRRKRKLSDDGAFISENHRINIARPDIFRQDPVNMIRLFHLADRHGLEFHPEAMQSLTRSLKLINADLRENPEANRLFLEILTSPRNPELILRRMNESGVLGKFIPDFGKIVAMMQFNMYHHYTVDEHLLRCIAVLSEIEHGELKTEHPLSNHLITTIKRDRNLLYVTLLLHDIAKGRPEDHSIAGARIARRLCPRFGLTPSETETVEWLVREHLTMSMVAQSRDLNDRKTIIDFADTVQTMERLKLLLILTVCDIKAVGPGIWNGWKGQLLRTLFYETELVLTGGFSELSRAARDKQAREALAERLSDWPKEERDAYLALPYTNYFLTVSLDDQVRHAHFIRDADQQGRALVTMAKPHAFEAVTEITVLAPDHPRLLSVITGACAAAGGNIVDAQIFTTSDGRALDTILISREFDTDDDERRRAERVGKVIEDVLSGKAHLPDMLAKRTKPKKAARAFKVEPRVEINNTLSNKFTVIEVEGLDRPGLLSELTGLISDLSLDIASAHITTFGEKVIDSFYVTDLVGHKISNATRQGNIKRKLLALLGAENGARTNGRSPQAAA
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Modifies, by uridylylation or deuridylylation the PII (glnB) regulatory protein (By similarity)(UniProt: Q8G312).

CATALYTIC ACTIVITY: UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII](UniProt: Q8G312).

SIMILARITY: Belongs to the glnD family(UniProt: Q8G312).

MUTATION: glnD encodes for a uridylyl transferase which is the primary sensor of nitrogen. The glnD mutant via signature-tagged transposon mutagenesis is attenuated in THP1 macrophages and HeLa cells. It supports the hypothesis that the concentration of glutamine in host cells is critical for the intracellular survival of Brucella (Foulongne et al., 2000).
COG COG2844O, under O: Posttranslational modification, protein turnover, chaperones
References
Foulongne et al., 2000: Foulongne V, Bourg G, Cazevieille C, Michaux-Charachon S, O'Callaghan D. Identification of Brucella suis genes affecting intracellular survival in an in vitro human macrophage infection model by signature-tagged transposon mutagenesis. Infection and immunity. 2000; 68(3); 1297-1303. [PubMed: 10678941].