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purD from Brucella melitensis bv. 1 str. 16M

Victor ID 1295
Gene Name purD from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) B rucella melitensis bv. 1 str. 16M
NCBI Gene ID 1197230
NCBI Protein GI 17987802
Locus Tag BMEI1519
Protein Accession NP_540436.1
Other Database IDs UniProtKB-ID: D0B5C4_BRUME
UniRef100: UniRef100_Q8YFK1
UniRef90: UniRef90_Q8YFK1
UniRef50: UniRef50_P52420
UniParc: UPI00000580D5
EMBL: GG703778
EMBL-CDS: EEW88598.1
RefSeq_NT: NC_003317.1
OMA: FSLMAFV
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 1562639
Gene Ending Position 1563922
Gene Strand (Orientation) +
Protein Name phosphoribosylamine--glycine ligase
DNA Sequence 
>gi|17986284:1562639-1563922 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
CATGAAAGTTCTGTTGATCGGTTCCGGTGGTCGCGAGCACGCCTTGGCATGGAAGCTTGCCGCCTCGCCC
CTGCTTGAAAAACTATATTGCGCGCTCGGCAATCCCGGTATCGCCGCTGTAGCGGAGCTTGCCGATATCG
GCGTGGACGACCATGCCGCGCTGATTGCCTTTGCCAAGGAAAAGCATATCGACCTCGTGGTCGTGGGGCC
GGAAGCGCCGCTTGTCGCCGGGCTTGCCGATGAAATGCGCGCTGAGGGCATCCGCGTCTTCGGCCCGTCC
AGGGCCGCAGCCCAGCTTGAAGGCTCGAAGGGCTTCACCAAGGATCTCTGCGCGCGCTTCAATATCCCGA
CCGGCGCTTATGGCCGTTTCAACAATGCGCCAAAGGCGAAAGCCTATATCCGCCAGCAGGGCGCGCCCAT
CGTGGTCAAGGCCGATGGGCTTGCCGCAGGCAAGGGCGTGGTTGTCGCCATGACGCTTCAGGAAGCACTT
GATGCGGTCGATTCCTGCTTTGAGGGCGCTTTCGGTGCGGCAGGCGCGGAAGTGGTGGTCGAGGAATTTC
TTGACGGTGAGGAAGCAAGCTTCTTCTGCATCTGCGATGGCAAGACGGCATTGCCGCTTGGCTCCGCGCA
GGATCACAAGCGCGTCGGCGATGGCGATACCGGCCCGAATACCGGCGGCATGGGCGCCTATGCGCCCGCC
CCGGTGATGACACCGGAAATGGTGGCGCGCACCATGCGCGAACTGATTGAACCCACCATGCGCGGCATGG
CGGAAATCGGCGCACCCTTTTCCGGCATTCTCTTTGCCGGGCTGATGATTACCAGCGATGGCCCGAAGCT
CATCGAATATAATACCCGTTTCGGCGATCCGGAATGCCAGGTATTGATGATGCGCCTCAACAGCGATCTG
CTTGCCCTCGTCAATGCTGCCGTTGACGGCAGACTTGATGAGGTTTCACTTGAATGGAAAGACGAACCGG
CGCTGACCGTAGTGATGGCCGCCGAAGGCTACCCGGCCAATGTGAAGAAGGGCAGCGTCATCCGCGACCT
CGAAAAGCTGGAAAGCATTGATGGCGTAAAACTGTTCCATGCGGGCACGGCGCTGAAGGACGGCGCCATC
GTCGCCAGCGGCGGGCGCGTCCTCAACATCACCGCTACGGCGGCAACCGTAGCGCAAGCGCAGGCGCGTG
CCTATGAGGCGCTGAAGCTCATCGACTGGCCGGAAGGTTTTTACCGTTCTGATATCGGCTGGCGCGCCGT
GGAGCGTGAGAAGGCTAATCGCTG
Protein Sequence 
>gi|17987802|ref|NP_540436.1| phosphoribosylamine--glycine ligase [Brucella melitensis bv. 1 str. 16M] MKVLLIGSGGREHALAWKLAASPLLEKLYCALGNPGIAAVAELADIGVDDHAALIAFAKEKHIDLVVVGPEAPLVAGLADEMRAEGIRVFGPSRAAAQLEGSKGFTKDLCARFNIPTGAYGRFNNAPKAKAYIRQQGAPIVVKADGLAAGKGVVVAMTLQEALDAVDSCFEGAFGAAGAEVVVEEFLDGEEASFFCICDGKTALPLGSAQDHKRVGDGDTGPNTGGMGAYAPAPVMTPEMVARTMRELIEPTMRGMAEIGAPFSGILFAGLMITSDGPKLIEYNTRFGDPECQVLMMRLNSDLLALVNAAVDGRLDEVSLEWKDEPALTVVMAAEGYPANVKKGSVIRDLEKLESIDGVKLFHAGTALKDGAIVASGGRVLNITATAATVAQAQARAYEALKLIDWPEGFYRSDIGWRAVEREKANR
Molecule Role Virulence factor
Molecule Role Annotation CATALYTIC ACTIVITY: ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide(UniProt: Q8G2B1).

PATHWAY: Nucleotide biosynthesis; IMP biosynthesis; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2(UniProt: Q8G2B1).

PATHWAY: Context: Purine biosynthesis(UniProt: Q8G2B1).

SIMILARITY: Belongs to the GARS family(UniProt: Q8G2B1).

SIMILARITY: Contains 1 ATP-grasp domain(UniProt: Q8G2B1).

MUTATION: Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALBc mouse model. It confirms the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages (Alcantara et al., 2004).

Like the purE mutant, a purD::Tn10 mutant has reduced survival in murine macrophages and reduced virulence in mice (Alcantara et al., 2004).
COG COG0151F, under F: Nucleotide transport and metabolism
References
Alcantara et al., 2004: Alcantara RB, Read RD, Valderas MW, Brown TD, Roop RM 2nd. Intact purine biosynthesis pathways are required for wild-type virulence of Brucella abortus 2308 in the BALB/c mouse model. Infection and immunity. 2004; 72(8); 4911-4917. [PubMed: 15271960].