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glyA from Brucella melitensis bv. 1 str. 16M

Victor ID 1266
Gene Name glyA from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1196903
NCBI Protein GI 17987475
Locus Tag BMEI1192
Protein Accession NP_540109.1
Other Database IDs UniProtKB-ID: GLYA_BRUME
UniRef100: UniRef100_Q8YGG7
UniRef90: UniRef90_A6X1Y9
UniRef50: UniRef50_A4SFY3
UniParc: UPI000021CAC6
EMBL: AE008917
EMBL-CDS: AAL52373.1
EMBL-CDS: AAL52372.1
RefSeq_NT: NC_003317.1
OMA: GRAYVTC
BioCyc: BMEL224914:BMEI1191-MONOMER
BioCyc: BMEL224914:BMEI1192-MONOMER
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 1235100
Gene Ending Position 1235852
Gene Strand (Orientation) -
Protein Name SERINE HYDROXYMETHYLTRANSFERASE
DNA Sequence 
>gi|17986284:1235100-1235852 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
ATCATGCCGCCGCGCGGACCGCGAAGCGATTTGTGCGTTGTCGTGGTGCAGACATGGGCATGGGGAACCG
GCGACGGATGCTGACCGCCAGCCACGAGACCGGCAATATGCGCCATATCGACCATGAGACAAGCGCCCAC
TTCATCGGCGATCTCGCGGAAACGCTTCCAGTCCCAGATACGCGAATAGGCCGTCCGCCTGCAAGGATAA
GCTTGGGCTTGTTTTCGCGTGCGAGGCGGGCGACTTCGTCCATGTCGAGCAGATGATCGTCCTTGCGCAC
GCCGTAGGACACGACATTGAACCACTTGCCGGACATATTGACCGGCGAACCGTGGGTCAGGTGGCCACCT
GAATTAAGGTCGAGGCCCATGAAGGTATCGCCTGGCTGAAGCAGCGCGAGGAAAACCGCCTGATTCATCT
GGCTGCCGGAATTCGGCTGAACATTGGCGAATTCGGCACCGAAAAGCTTCTTGGCGCGTTCGATCGCCAG
TTCTTCCACGACATCGACATACTGGCAGCCGCCATAATAGCGCTTGCCCGGATAGCCTTCGGCATATTTG
TTGGTGAGGATGGAACCCTGTGCTTCCAGAACAGCGCGCGAAACGATGTTTTCCGAAGCGATCAGCTCGA
TTTCATGGCGCTGGCGACCAAGTTCGTTGCGGATTGCACCGAAAATCTCGGGATCGATGTCCTCGAGGCT
TGCATTGAAGAAAACGTCCGAAGACGCTTTCGTGGCGGCGTTGGCTTGAGACA
Protein Sequence 
>gi|17987475|ref|NP_540109.1| serine hydroxymethyltransferase [Brucella melitensis bv. 1 str. 16M] MSQANAATKASSDVFFNASLEDIDPEIFGAIRNELGRQRHEIELIASENIVSRAVLEAQGSILTNKYAEGYPGKRYYGGCQYVDVVEELAIERAKKLFGAEFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVRKDDHLLDMDEVARLARENKPKLILAGGRPIRVSGTGSVSARSPMKWALVSWSIWRILPVSWLAVSIRRRFPMPMSAPRQRTNRFAVRAAA
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Interconversion of serine and glycine(UniProt: Q8G1F1).

CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine(UniProt: Q8G1F1).

COFACTOR: Pyridoxal phosphate (By similarity)(UniProt: Q8G1F1).

PATHWAY: Key enzyme in the biosynthesis of purines, lipids, hormones and other components(UniProt: Q8G1F1).

SUBUNIT: Homotetramer (By similarity)(UniProt: Q8G1F1).

SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: Q8G1F1).

SIMILARITY: Belongs to the SHMT family(UniProt: Q8G1F1).

MUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis glyA gene is required for intracellular multiplication in human macrophage THP-1 cells (Kohler et al., 2002).
COG COG0112E, under E: Amino acid transport and metabolism
References
Kohler et al., 2002: Kohler S, Foulongne V, Ouahrani-Bettache S, Bourg G, Teyssier J, Ramuz M, Liautard JP. The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. Proceedings of the National Academy of Sciences of the United States of America. 2002 Nov 26; 99(24); 15711-6. [PubMed: 12438693 ].