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purL from Brucella melitensis bv. 1 str. 16M

Victor ID 1265
Gene Name purL from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1196838
NCBI Protein GI 17987410
Locus Tag BMEI1127
Protein Accession NP_540044.1
Other Database IDs UniProtKB-ID: PURL_BRUME
UniRef100: UniRef100_B2S573
UniRef90: UniRef90_A9MAL4
UniRef50: UniRef50_Q8UEB0
UniParc: UPI0000132B29
EMBL: AE008917
EMBL-CDS: AAL52308.1
RefSeq_NT: NC_003317.1
GenomeReviews: AE008917_GR
KEGG: bme:BMEI1127
HOGENOM: HBG311214
OMA: YGNSFGV
ProtClustDB: PRK01213
BioCyc: BMEL224914:BMEI1127-MONOMER
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 1169664
Gene Ending Position 1171919
Gene Strand (Orientation) +
Protein Name phosphoribosylformylglycinamidine synthase II
DNA Sequence 
>gi|17986284:1169664-1171919 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
GGTGCCACAGCCTGCGACGGGGTTCCCCATTTCCATGACTATTTCCAATACGCGAGACATCACGCCGGAA
CTGATCGAAGCGCATGGTCTTAAGCCGGACGAGTATCAGCGCATTCTTGAGCTGATCGGGCGCGAGCCGA
CTTTTACCGAACTCGGCATTTTCTCCGCCATGTGGAATGAGCACTGCTCCTACAAGTCCTCCAAGAAATG
GCTACGCACACTTCCGACCAGCGGACCGCGCGTCATTCAAGGCCCCGGTGAAAATGCAGGCGTGGTCGAT
ATTGGCGATGGCGATTGCGTCGTCTTCAAGATGGAAAGCCACAATCACCCCTCCTATATCGAGCCTTATC
AGGGTGCCGCGACCGGCGTGGGCGGAATCTTGCGCGACGTGTTCACCATGGGCGCGCGCCCCGTTGCGGC
AATGAATGCGCTGCGCTTTGGCGAACCCGACCATCCAAAAACCCGCCACCTCGTCTCGGGCGTTGTTTCC
GGCGTCGGCGGCTATGGCAATGCCTTCGGCGTGCCGACTGTCGGTGGCGAGGTGAATTTCGACAAGCGCT
ATAACGGCAATATCCTCGTCAATGCCTTTGCTGCCGGTCTTGCCCGGCATGATGGCATTTTCCTGTCGGA
AGCCGAGGGCGTCGGCCTGCCGGTCGTCTATCTGGGTGCAAAGACTAGCCGCGATGGTGTTGGCGGCGCA
ACAATGGCATCGGCTGAATTCGACGAGTCGATCGAGGAAAAGCGCCCCACCGTTCAGGTGGGCGATCCTT
TCACCGAAAAATGCCTGCTTGAAGCCTGCCTTGAACTCATGGCTTCCGGTGCGGTCATCGCCATTCAGGA
CATGGGCGCTGCCGGCCTCACCTGCTCGGCGGTCGAGATGGGTGCCAAGGGCGATCTCGGCATCGAACTC
ATTCTCGATCATGTTCCCGTTCGCGAAGAGAACATGACCGCCTATGAAATGATGCTTTCGGAAAGCCAGG
AGCGCATGCTCATGGTGCTGAAGCCGGAAAAGGAAGCAGAAGCGCAGGCTATCTTCCGCAAATGGGGCCT
TGATTTCGCGATTGTCGGCAAGACCACGGACGATCTTCGTTTCCGCGTCATCCATCAGGGGGAAGAGGTC
GCCAATCTGCCGATCAAGGATCTGGGCGACGAAGCGCCGGAATATGATCGCCCGTGGATGGAGCCGGGCA
AGCATGCGCCGCTGCCTGCCAGCAATGTGCCGCAGGTGGAAGACTATTCCGCAGCCCTGCTGAAGCTGAT
CGGCTCGCCCGATCTTTCCTCGCGCCGCTGGGTCTATGAACAGTACGACACGCTCATTCAGGGCAATTCC
TTGCAGGTTCCGGGCGGCGACGCCGGTGTAATCCGTGTCGAAGGCCATGAAACCAAGGCTCTCGCCTTCT
CTTCCGACGTGACGCCGCGCTATTGCGAAGCCGATCCGTTTGAAGGCGGCAAGCAGGCCGTTGCCGAATG
CTGGCGCAACATCACGGCGACCGGCGCGGAGCCGCTGGCCTCGACCGATAATCTGAACTTCGGCAATCCC
GAAAAGCCGGAAATCATGGGCCAGCTCGTCAAGGCTATCGAAGGCATTGGCGAGGCCTGCCGCGCACTCG
ACTTCCCGATCGTGTCCGGCAATGTGTCGCTTTACAATGAGACCAACGGCCAGGCCATTCTGCCAACGCC
AACGATTGCGGGTGTTGGCCTCCTTCCCGACTGGTCGCAAATGGCGAAGATCGGCGGCATGCAGGATGGC
GATACGCTGGTTCTGCTCGGTGGTGACGGAACGCATCTCGGCCAGTCGGTCTATCTGCGTGACCTTTTCG
ACCGCGCGGACGGTCCTGCGCCTTTTGTCGATCTGGCGCTTGAAAAGCGCAATGGCGAATTTGTCCGTTC
TGCCATTCGCAACGGTCAGGTGACGGCCTGCCATGATCTTTCCGATGGCGGTCTGGCGATTGCCGTTGCC
GAAATGGCGATCAAGTCCGGCAAAGGTGCGACGCTGGACGCTGGTGACGGCCTGCCGCACGCCCTGCTTT
TCGGTGAAGATCAGGCACGCTATGTTATTTCCGCCACGCCTGAAATGGCAAAGCTCATCGCGCTTAACGC
GGAAGGCGCAGGCGTTCCATTCCGCATTTTGGGTACAGTTGGCGGCGACCGGCTGAAAATCAGCAAGAAT
GTCGATGTCAGCGTGGCCGATCTGACGCAGGCTTATGAAGGTTGGTTCCCGAACTTCATGAACGGCGAAC
TGACCGGCAATAACTG
Protein Sequence 
>gi|17987410|ref|NP_540044.1| phosphoribosylformylglycinamidine synthase II [Brucella melitensis bv. 1 str. 16M] MPQPATGFPISMTISNTRDITPELIEAHGLKPDEYQRILELIGREPTFTELGIFSAMWNEHCSYKSSKKWLRTLPTSGPRVIQGPGENAGVVDIGDGDCVVFKMESHNHPSYIEPYQGAATGVGGILRDVFTMGARPVAAMNALRFGEPDHPKTRHLVSGVVSGVGGYGNAFGVPTVGGEVNFDKRYNGNILVNAFAAGLARHDGIFLSEAEGVGLPVVYLGAKTSRDGVGGATMASAEFDESIEEKRPTVQVGDPFTEKCLLEACLELMASGAVIAIQDMGAAGLTCSAVEMGAKGDLGIELILDHVPVREENMTAYEMMLSESQERMLMVLKPEKEAEAQAIFRKWGLDFAIVGKTTDDLRFRVIHQGEEVANLPIKDLGDEAPEYDRPWMEPGKHAPLPASNVPQVEDYSAALLKLIGSPDLSSRRWVYEQYDTLIQGNSLQVPGGDAGVIRVEGHETKALAFSSDVTPRYCEADPFEGGKQAVAECWRNITATGAEPLASTDNLNFGNPEKPEIMGQLVKAIEGIGEACRALDFPIVSGNVSLYNETNGQAILPTPTIAGVGLLPDWSQMAKIGGMQDGDTLVLLGGDGTHLGQSVYLRDLFDRADGPAPFVDLALEKRNGEFVRSAIRNGQVTACHDLSDGGLAIAVAEMAIKSGKGATLDAGDGLPHALLFGEDQARYVISATPEMAKLIALNAEGAGVPFRILGTVGGDRLKISKNVDVSVADLTQAYEGWFPNFMNGELTGNN
Molecule Role Virulence factor
Molecule Role Annotation CATALYTIC ACTIVITY: ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H(2)O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate(UniProt: Q8G183).

PATHWAY: Nucleotide biosynthesis; IMP biosynthesis; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1(UniProt: Q8G183).

PATHWAY: Context: Purine biosynthesis(UniProt: Q8G183).

SUBUNIT: Heterodimer of two subunits, purQ and purL(UniProt: Q8G183).

SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: Q8G183).

SIMILARITY: Belongs to the FGAMS family(UniProt: Q8G183).

MUTATION: Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALBc mouse model. It confirms the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages (Alcantara et al., 2004). Studies with B melitensis purE and B abortus purL mutants, which are purine auxotrophs, and B suis aroC mutants, which can not synthesize aromatic amino acids, indicate that the brucellae also face signicant nutrient limitation during their prolonged residence in host macrophages (Alcantara et al., 2004).
COG COG0046F, under F: Nucleotide transport and metabolism
References
Alcantara et al., 2004: Alcantara RB, Read RD, Valderas MW, Brown TD, Roop RM 2nd. Intact purine biosynthesis pathways are required for wild-type virulence of Brucella abortus 2308 in the BALB/c mouse model. Infection and immunity. 2004; 72(8); 4911-4917. [PubMed: 15271960].