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uvrA from Brucella melitensis bv. 1 str. 16M

Victor ID 1251
Gene Name uvrA from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1196589
NCBI Protein GI 17987161
Locus Tag BMEI0878
Protein Accession NP_539795.1
Other Database IDs UniProtKB-ID: D0B9E1_BRUME
UniRef100: UniRef100_Q8YHC4
UniRef90: UniRef90_Q2YPX5
UniRef50: UniRef50_Q2YPX5
UniParc: UPI0000057E58
EMBL: GG703780
EMBL-CDS: EEW86797.1
RefSeq_NT: NC_003317.1
OMA: GAIKGWD
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 908166
Gene Ending Position 911090
Gene Strand (Orientation) -
Protein Name excinuclease ABC subunit A
DNA Sequence 
>gi|17986284:908166-911090 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
TTTACTCGGCAGCTTGGGAGCTGCGCTTGGGCCGGCGTTCCAGCAGCTCCTTGAGGAATTGGCCGGTATA
AGACCGCTTCTCCTGCACAATGTCTTCCGGACGGCCTACGGCAACAATCTCGCCGCCGCCGTCGCCACCT
TCCGGTCCAAGGTCGATCACCCAGTCGGCAGTCTTGATGACTTCCAGATTATGCTCGATCACAACGACCG
TGTTTCCTTGCTCCACCAGTTCGTGCAGCACTTCGAGCAGTTTTGCTACGTCGTGGAAATGCAGGCCGGT
GGTGGGTTCGTCAAGAATATAGAGCGTGCGGCCAGTTGCGCGGCGCGACAGTTCCTTGGCAAGCTTCACA
CGCTGCGCTTCGCCGCCGGAAAGCGTCGTCGCCTGCTGGCCGACCTTGATGTAACCGAGGCCGACCTTGA
CGAGCGTTTCCAGCTTGTCGCGCACGGCCGGCACGGCGGAGAAGAATTCAGCACCTTCTTCCACTGTCAT
GTCGAGAACATCGGCAATGGACTTGCCCTTGAACAGCACATCCAGTGTTTCGCGGTTATACCGCTTGCCG
TGGCAGACATCGCAGGTCACATAAACGTCTGGCAGGAAGTGCATCTCGATCTTGATGACGCCGTCGCCCT
GACATGCCTCGCAGCGCCCGCCCTTGATGTTGAAGGAAAAGCGGCCCGGCTGATAGCCGCGCGCCTTGGC
TTCCGGCAGGCCCGCAAACCAGTCGCGGATTGGTGTGAACGCACCGGTATAGGTGGCCGGGTTGGAGCGC
GGGGTGCGCCCGATCGGTGACTGGTCAATGTCAATCACCTTGTCGAGAAATTCCAGCCCCTCTATCCGGT
CGTGTTCAGCCGGATGCTCACGCGACCCCATGATGCGGCGCGACGCAGCCTTGAACAACGTCTCGATCAG
GAAGGTGGACTTGCCTCCGCCTGAAACGCCCGTGACAGCGGTGAAGGTGCCGAGTGGAATATCGGCTGAA
ACATTCTTCAGGTTGTTGCCCCGAGCGCCCACGACACGCAGCCGCTTGGTCTTGGAAATTTTGCGACGCT
CGGCCGGGACGGCAACTTCCATCGCGCCGGACAGATATTTGCCTGTCAGCGAATTCGTATTTGCCATGAT
ATCCTGGGGCGAGCCCTGCGCAATCACCTTGCCGCCATGCACGCCCGCTGCCGGGCCGATATCGACCACA
TAATCGGCGGTGAGGATTGCGTCCTCATCATGCTCGACCACGATGACCGTATTACCGAGATCGCGCAGGT
GGCGCAGCGTGTCGAGCAGGCGGGCATTATCACGCTGATGCAAACCAATGGATGGTTCATCCAGCACGTA
AAGCACGCCGGTGAGGCCCGAGCCGATCTGCGAGGCAAGGCGGATACGCTGGCTTTCGCCGCCAGAGAGC
GTGCCGGAATTGCGCGCCAGCGTCAGGTAGTCGAGCCCCACATCGTTGAGGAATTGCAGGCGCTCGCGAA
TTTCTTTCAGGATGCGCGCTGCAATTTCACGCTGCTTTTCATTAAAGCTGCCATCGATGTCGCGGAACCA
GGCATCGGCCTTGCGGATCGACATTTCGGTGATCTCGCCAATATGTTTTTTGCCGATCTTTACGGACAGG
GCTTCCGGCTTCAGGCGATAGCCATTGCAGGCGGGGCAGGGGGTGGAGGCCATGAAGCGCTCGATTTCCT
CACGCGACCATGCCGAATCGGTTTCCTTCCAGCGGCGTTCCAGATTGGGGATCACCCCCTCGAAAGGTTT
CGTGGTTTGATAAGACCGCAGCCCGTCGTCATAATGGAAGGTAATTTCCCTGCCCTTGGTGCCATAGAGA
ATGGCTTGCCGGGCCTCTTCGGAAAGTTCCGACCAGCGCGCGCTTACCTTGAAACCATAGGCCTTGCCCA
AGGCTTCCAGCGTCTGGTTGTAATAGGGCGACGAGGAGCGCGCCCATGGTGCAACCGCGCCGTCTTTCAG
CGCTGCGCTTTCATCGGGAATAATAAGGTTTGGATCGATGGCCTGCTGCGTGCCGAGACCGTCGCAGGTG
GGGCAGGCACCAAACGGGTTGTTGAACGAGAACAGGCGCGGCTCGATCTCAGGAATGGTGAAACCGGAAA
CCGGGCAGGCAAATTTTTCCGAGAAAAGAATGCGCTCATGCGTTTCGTTGGCTGATTTATTGGCTGAACC
GCCTTCCGCCGTTTCTCCCACTGGCAGGGGCTTGTCGGCAAATTCGGCAATGGCCAGACCATCCGCAAGC
TTGAGACAGGTTTCGAGACTGTCTGCAAGACGTGTGGACAAATCCGGGCGCACCACCACGCGGTCTACCA
CTACGTCGATATCGTGCTTGTATTTCTTGTCGAGTGGCGGAACGTCGGCGATCTCATAGAAGGTGCCGTC
CACCTTCACGCGCTGGAAGCCCTTTTTCTGAAGTTCGGCGAGTTCCTTCCTGTATTCGCCCTTACGCCCG
CGCACGATAGGCGCAAGGATATAAAGACGTGTGCCTTCCTCCAGTGCGATGACCCTATCGACCATCTGGC
TCACCGTCTGGCTTTCGATCGGAAGGCCGGTAGCAGGAGAATAGGGAATGCCCACACGCGCAAAGAGCAG
GCGCATATAGTCGTAGATTTCGGTGACTGTGCCCACCGTCGAACGCGGATTGCGGCTTGTCGTCTTCTGC
TCGATGGAAATGGCGGGCGACAGGCCGTCAATCTGGTCGACATCCGGTTTCTGCATCATTTCGAGAAACT
GGCGCGCATAGGCTGAAAGGCTTTCGACATAGCGGCGCTGGCCTTCGGCATAGATCGTATCGAAAGCAAG
CGAGGATTTGCCCGAGCCAGAAAGCCCCGTCATCACGATCAGCTTGTCGCGCGGCAGATCGAGATCGACA
TTTTTCAGATTGTGCTCGCGTGCGCCACGTATGGAAATGAATTTCTGATCGCTCA
Protein Sequence 
>gi|17987161|ref|NP_539795.1| excinuclease ABC subunit A [Brucella melitensis bv. 1 str. 16M] MSDQKFISIRGAREHNLKNVDLDLPRDKLIVMTGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLEMMQKPDVDQIDGLSPAISIEQKTTSRNPRSTVGTVTEIYDYMRLLFARVGIPYSPATGLPIESQTVSQMVDRVIALEEGTRLYILAPIVRGRKGEYRKELAELQKKGFQRVKVDGTFYEIADVPPLDKKYKHDIDVVVDRVVVRPDLSTRLADSLETCLKLADGLAIAEFADKPLPVGETAEGGSANKSANETHERILFSEKFACPVSGFTIPEIEPRLFSFNNPFGACPTCDGLGTQQAIDPNLIIPDESAALKDGAVAPWARSSSPYYNQTLEALGKAYGFKVSARWSELSEEARQAILYGTKGREITFHYDDGLRSYQTTKPFEGVIPNLERRWKETDSAWSREEIERFMASTPCPACNGYRLKPEALSVKIGKKHIGEITEMSIRKADAWFRDIDGSFNEKQREIAARILKEIRERLQFLNDVGLDYLTLARNSGTLSGGESQRIRLASQIGSGLTGVLYVLDEPSIGLHQRDNARLLDTLRHLRDLGNTVIVVEHDEDAILTADYVVDIGPAAGVHGGKVIAQGSPQDIMANTNSLTGKYLSGAMEVAVPAERRKISKTKRLRVVGARGNNLKNVSADIPLGTFTAVTGVSGGGKSTFLIETLFKAASRRIMGSREHPAEHDRIEGLEFLDKVIDIDQSPIGRTPRSNPATYTGAFTPIRDWFAGLPEAKARGYQPGRFSFNIKGGRCEACQGDGVIKIEMHFLPDVYVTCDVCHGKRYNRETLDVLFKGKSIADVLDMTVEEGAEFFSAVPAVRDKLETLVKVGLGYIKVGQQATTLSGGEAQRVKLAKELSRRATGRTLYILDEPTTGLHFHDVAKLLEVLHELVEQGNTVVVIEHNLEVIKTADWVIDLGPEGGDGGGEIVAVGRPEDIVQEKRSYTGQFLKELLERRPKRSSQAAE
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by uvrB, the uvrA molecules dissociate (By similarity)(UniProt: Q8G0I9).

SUBUNIT: Forms a heterotetramer with uvrB during the search for lesions (By similarity)(UniProt: Q8G0I9).

SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: Q8G0I9).

SIMILARITY: Belongs to the ABC transporter family. UvrA subfamily(UniProt: Q8G0I9).

SIMILARITY: Contains 2 ABC transporter domains(UniProt: Q8G0I9).

MUTATION: B. abortus urvA and recA mutants exhibited greater sensitivity than the wild-type strain. Mutant strains carrying inactivated uvrA genes are typically less sensitive than recA mutants because there is only the loss of the nucleotide excision repair system, just one subset of the larger repair networks. However, it was found that the recA mutant conferred only a modest sensitivity to UV, substantially less sensitive than the uvrA mutant. High basal recA expression was observed in the uvrA repair mutant. The B abortus recA mutant exhibited a nearly fourfold decline in survival to murine peritoneal macrophages but nominal sensitivity for the uvrA and radA repair mutants (Roux et al., 2006).
COG COG0178L, under L: Replication, recombination and repair
References
Roux et al., 2006: Roux CM, Booth NJ, Bellaire BH, Gee JM, Roop RM 2nd, Kovach ME, Tsolis RM, Elzer PH, Ennis DG. RecA and RadA proteins of Brucella abortus do not perform overlapping protective DNA repair functions following oxidative burst. Journal of bacteriology. 2006; 188(14); 5187-5195. [PubMed: 16816190].