Victors

lon from Brucella melitensis bv. 1 str. 16M
Victor ID	1250
Gene Name	lon from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism)	Brucella melitensis bv. 1 str. 16M
NCBI Gene ID	1196587
NCBI Protein GI	17987159
Locus Tag	BMEI0876
Protein Accession	NP_539793.1
Other Database IDs	UniProtKB-ID: LON_BRUME UniRef100: UniRef100_Q8YHC6 UniRef90: UniRef90_Q2YPX3 UniRef50: UniRef50_A8HYF7 UniParc: UPI000021CACE EMBL: AE008917 EMBL-CDS: AAL52057.1 RefSeq_NT: NC_003317.1 MEROPS: S16.001 GenomeReviews: AE008917_GR KEGG: bme:BMEI0876 NMPDR: fig\|224914.1.peg.875 HOGENOM: HBG566281 OMA: IQKMSKV ProtClustDB: CLSK864999 BioCyc: BMEL224914:BMEI0876-MONOMER
Taxonomy ID	224914
Chromosome No	I
Gene Starting Position	905052
Gene Ending Position	907523
Gene Strand (Orientation)	+
Protein Name	ATP-DEPENDENT PROTEASE LA
DNA Sequence	>gi\|17986284:905052-907523 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence GTTGGGTCGGAAGGCCCGAGAAAGGACTAGTGTTATGACGGGTATTGAACAGAAAACACCGGTTGGTGGT TCTGAAACGGGTGGTGCGGACGGGCTCTATGCTGTCTTGCCGCTGCGTGACATCGTCGTCTTTCCCCATA TGATCGTCCCGCTTTTTGTGGGCCGCGAAAAATCCATTCGCGCGCTTGAAGAAGTGATGGGCGTTGACAA GCAGATATTGCTTGCCACCCAGAAGAACGCTGCCGATGACGATCCGGCGCCGGACGCGATCTATGAGATT GGTACGATCGCCAATGTGTTGCAGCTTCTGAAACTGCCGGACGGCACCGTCAAGGTGCTGGTCGAAGGCA CGGCACGCGCCAAAATTTCCAAGTTTACCGATCGTGAAGATTATCACGAGGCTTATGCTGCGGCTCTGCA GGAGCCGGAGGAAGATGCTGTCGAGATCGAGGCACTGGCCCGCTCGGTGGTTTCCGACTTTGAAAATTAC GTCAAGCTGAACAAGAAGATTTCGCCGGAAGTGGTTGGCACGGCCAGCCAGATCGACGATTATTCCAAGC TTGCCGATACGGTTGCCTCGCACCTTGCGATCAAAATCCCTGAAAAGCAGGAAATGCTGTCGGTTCTTTC GGTGCGCGAGCGCCTTGAGAAAGCCCTTTCCTTCATGGAAGCCGAAATTTCTGTTCTACAGGTTGAGAAG CGTATTCGCAGCCGCGTCAAGCGCCAGATGGAGAAGACGCAGCGCGAATATTATCTCAATGAGCAGATGA AGGCGATCCAGAAGGAGCTTGGCGACAGTGAGGACGGCCGTGATGAAGTGGCCGAAATCGAAGAGCGCAT CACCAAGACCAAGCTCAGCAAGGAAGCGCGCGAAAAAGCTCTGGCCGAGCTGAAGAAACTGCGCAGCATG AGCCCGATGTCCGCTGAAGCGACGGTGGTTCGCAATTATCTCGACTGGTTGCTCTCCATTCCATGGGGCA AGAAGTCGAAGGTGAAGCAGGATCTGAACTTTGCGCAGGAAGTGCTGGATGCGGAGCATTTCGGCCTTGG CAAGGTCAAGGAACGCATCGTCGAATATCTGGCGGTGCAGGCCCGTTCGACCAAGATCAAGGGTCCGATC CTCTGCCTCGTTGGCCCTCCCGGCGTCGGCAAGACCTCGCTTGCGCGCTCGATTGCCAAGGCAACGGGCC GCGAATATGTCCGCATGTCGCTTGGCGGCGTACGCGACGAGGCTGAAATCCGCGGTCATCGCCGCACCTA TATCGGCTCGATGCCCGGCAAGGTCATCCAGTCGATGAAGAAGGCGAAGAAGTCCAATCCGCTTTTCCTG CTCGATGAAATCGACAAGATGGGCCAGGATTTCCGCGGCGATCCGTCTTCGGCCATGCTGGAGGTGCTGG ACCCGAAACAGAACGCGACCTTCATGGATCACTACCTTGAAGTTGAGTATGATCTATCGAACGTCATGTT CGTGACGACCGCCAATACGATGAATATTCCCGGTCCACTTCTGGATCGTATGGAGATCATCCGTATCGCC GGTTACACGGAAGACGAAAAGCTGGAGATCGCCAAGCGGCACCTGTTGCCGAAGGCCATCAAGGACCATG CCCTGCAACCGAAGGAGTTTTCGGTTACGGAAGATGCGCTGCGCAACGTTATCCGCCATTATACGCGGGA AGCGGGCGTGCGTAGCCTTGAACGCGAGGTGATGACCCTTGCGCGCAAGGCCGTGACGGAAATCCTGAAG ACGAAGAAGAAGTCGGTAAAGATTACCGACAAGAACCTCTCCGATTACCTTGGTGTGGAGAAGTTCCGCT TCGGTCAGATCGACGGTGAAGATCAGGTGGGTGTCGTGACTGGCCTTGCCTGGACCGAAGTCGGCGGTGA GCTTTTGACCATCGAAGGCGTCATGATGCCGGGTAAGGGCCGCATGACGGTTACGGGTAATCTCCGTGAC GTGATGAAGGAATCGATTTCGGCCGCGGCATCCTATGTCCGCTCGCGCGCGATCGATTTCGGCATCGAGC CTCCGCTGTTCGACAAGCGCGATATCCACGTGCACGTGCCGGAAGGCGCGACGCCGAAGGATGGTCCTTC TGCCGGTATTGCCATGGTTACGGCCATCGTCTCCGTGCTGACGGGTATTCCCGTTCGCAAGGACATCGCC ATGACGGGTGAAGTCACGTTGCGCGGTCGGGTTCTGCCAATCGGCGGGTTGAAGGAAAAGCTGCTTGCGA CCTTGCGCGGCGGTATCAAGAAGGTTCTGATCCCGGAAGAGAACGCCAAGGATCTGGCGGAAATCCCGGA CAATGTGAAGAACAATCTTGAGATCGTTCCGGTATCCCGCGTCGGTGAAGTGCTGAAGCACGCGCTCGTG CGCCAGCCTGAACCGATTGAATGGACCGAGCAGGAGAATCCCACTGCCGTGCCTCCGGTGGAGGATGAAG CAGGGGCTTCGCTGGCCCATTA
Protein Sequence	>gi\|17987159\|ref\|NP_539793.1\| ATP-dependent protease LA [Brucella melitensis bv. 1 str. 16M] MGRKARERTSVMTGIEQKTPVGGSETGGADGLYAVLPLRDIVVFPHMIVPLFVGREKSIRALEEVMGVDKQILLATQKNAADDDPAPDAIYEIGTIANVLQLLKLPDGTVKVLVEGTARAKISKFTDREDYHEAYAAALQEPEEDAVEIEALARSVVSDFENYVKLNKKISPEVVGTASQIDDYSKLADTVASHLAIKIPEKQEMLSVLSVRERLEKALSFMEAEISVLQVEKRIRSRVKRQMEKTQREYYLNEQMKAIQKELGDSEDGRDEVAEIEERITKTKLSKEAREKALAELKKLRSMSPMSAEATVVRNYLDWLLSIPWGKKSKVKQDLNFAQEVLDAEHFGLGKVKERIVEYLAVQARSTKIKGPILCLVGPPGVGKTSLARSIAKATGREYVRMSLGGVRDEAEIRGHRRTYIGSMPGKVIQSMKKAKKSNPLFLLDEIDKMGQDFRGDPSSAMLEVLDPKQNATFMDHYLEVEYDLSNVMFVTTANTMNIPGPLLDRMEIIRIAGYTEDEKLEIAKRHLLPKAIKDHALQPKEFSVTEDALRNVIRHYTREAGVRSLEREVMTLARKAVTEILKTKKKSVKITDKNLSDYLGVEKFRFGQIDGEDQVGVVTGLAWTEVGGELLTIEGVMMPGKGRMTVTGNLRDVMKESISAAASYVRSRAIDFGIEPPLFDKRDIHVHVPEGATPKDGPSAGIAMVTAIVSVLTGIPVRKDIAMTGEVTLRGRVLPIGGLKEKLLATLRGGIKKVLIPEENAKDLAEIPDNVKNNLEIVPVSRVGEVLKHALVRQPEPIEWTEQENPTAVPPVEDEAGASLAH
Molecule Role	Virulence factor
Molecule Role Annotation	FUNCTION: Degrades short-lived regulatory and abnormal proteins in presence of ATP. Hydrolyzes two ATPs for each peptide bond cleaved in the protein substrate (By similarity)(UniProt: Q8G0I7). CATALYTIC ACTIVITY: Hydrolysis of proteins in presence of ATP(UniProt: Q8G0I7). SUBUNIT: Homotetramer (By similarity)(UniProt: Q8G0I7). SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: Q8G0I7). SIMILARITY: Belongs to the peptidase S16 family(UniProt: Q8G0I7). SIMILARITY: Contains 1 Lon domain(UniProt: Q8G0I7). MUTATION: In contrast to the parent strain, the Brucella abortus lon mutant, was impaired in its capacity to form isolated colonies on solid medium at 41 degrees C and displayed an increased sensitivity to killing by puromycin and H₂O2. Brucella abortus Lon homologue functions as a stress response protease that is required for wild-type virulence during the initial stages of infection in the mouse model, but is not essential for the establishment and maintenance of chronic infection in this host (Robertson et al., 2000). Both single lon or clpA mutations had comparable effects on growth inhibition, suggesting that the concerned proteases Lon and ClpAP both degrade a number of specific proteins, but are also both involved in general degradation of abnormal proteins. Compared to the single mutants, the double mutant lon clpA was highly sensitive to canavanine. One possible explanation for this observation is that both proteases can substitute for each other to a large extent during bacterial growth. Hence, simultaneous inactivation or decrease in activation of both proteases, either by direct mutation or by elimination of the regulatory component ClpA, strongly increased growth inhibition (Robertson et al., 2000).
COG	COG0466O, under O: Posttranslational modification, protein turnover, chaperones
References	*Robertson et al., 2000:* Robertson GT, Kovach ME, Allen CA, Ficht TA, Roop RM 2nd. The Brucella abortus Lon functions as a generalized stress response protease and is required for wild-type virulence in BALB/c mice. Molecular microbiology. 2000; 35(3); 577-588. [PubMed: 10672180].


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lon from Brucella melitensis bv. 1 str. 16M