Search: for Help
About
Introduction
Statistics
Your PHIDIAS
Register or Login
Philert
Submission
Curated Data
Victors
BBP (Brucella)
Phinfo
Phinet
HazARD
Data Analysis
Phigen
Pacodom
BLAST
Help & Documents
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

hfq from Brucella melitensis bv. 1 str. 16M

Victor ID 1249
Gene Name hfq from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1196583
NCBI Protein GI 17987155
Locus Tag BMEI0872
Protein Accession NP_539789.1
Other Database IDs UniProtKB-ID: D0B9D5_BRUME
UniRef100: UniRef100_B2S5W3
UniRef90: UniRef90_B3PYU7
UniRef50: UniRef50_B3PYU7
UniParc: UPI0000057E52
EMBL: GG703780
EMBL-CDS: EEW86791.1
RefSeq_NT: NC_003317.1
OMA: STISPMK
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 900418
Gene Ending Position 900654
Gene Strand (Orientation) +
Protein Name RNA-binding protein Hfq
DNA Sequence 
>gi|17986284:900418-900654 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
AATGGCTGAACGATCGCAAAATCTACAAGACCTCTTTCTGAATTCTGTCCGCAAGCAGAAGATTTCTCTA
ACGATCTTCCTTATCAATGGCGTGAAATTGACCGGCATTGTAACGTCTTTCGATAATTTCTGCGTGCTTC
TGCGTCGTGATGGTCATTCGCAGCTTGTTTACAAGCATGCTATTTCGACAATCATGCCGAGCCAGCCTGT
ACAGATGTTTGAAGGCGAGGAAGCCTG
Protein Sequence 
>gi|17987155|ref|NP_539789.1| RNA-binding protein Hfq [Brucella melitensis bv. 1 str. 16M] MAERSQNLQDLFLNSVRKQKISLTIFLINGVKLTGIVTSFDNFCVLLRRDGHSQLVYKHAISTIMPSQPVQMFEGEEA
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: RNA-binding protein that stimulates the elongation of poly(A) tails (By similarity)(UniProt: P0A3G8).

SIMILARITY: Belongs to the hfq family(UniProt: P0A3G8).

MUTATION: hfq encodes for the RNA binding protein host factor I (HF-I). The hfq knock out strain has been showed a reduced growth rate and is unable to utilize glucose as a sole carbon source(Sonnleitner et al., 2003).

hfq is required for the efficient translation of the stationary-phase sigma factor RpoS in many bacteria, and a Brucella abortus hfq mutant displays a phenotype in vitro, which suggests that it has a generalized defect in stationary-phase physiology. The inability of the B. abortus hfq mutant to survive and replicate in a wild-type manner in cultured murine macrophages, and the profound attenuation displayed by this strain and its B melitensis counterpart in experimentally infected animals indicate that stationary -phase physiology plays an essential role in the capacity of the brucellae to establish and maintain long-term intracellular residence in host macrophages (Roop et al., 2003).

In contrast to B abortus 2308, the isogenic hfq and bacA mutants remained in acidic, LAMP-1 phagosomes and failed to initiate intracellular replication (Roop et al., 2003).

A hfq mutant of B abortus was eliminated from mouse spleens more rapidly than the wild type (Roop et al., 2003).
COG COG1923R, under R: General function prediction only
References
Roop et al., 2003: Roop RM 2nd, Gee JM, Robertson GT, Richardson JM, Ng WL, Winkler ME. Brucella stationary-phase gene expression and virulence. Annual review of microbiology. 2003; 57; 57-76. [PubMed: 12730323].
Sonnleitner et al., 2003: Sonnleitner E, Hagens S, Rosenau F, Wilhelm S, Habel A, Jäger KE, Bläsi U. Reduced virulence of a hfq mutant of Pseudomonas aeruginosa O1. Microbial pathogenesis. 2003; 35(5); 217-228. [PubMed: 14521880].