Victors

recA from Brucella melitensis bv. 1 str. 16M
Victor ID	1245
Gene Name	recA from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism)	Brucella melitensis bv. 1 str. 16M
NCBI Gene ID	1196498
NCBI Protein GI	17987070
Locus Tag	BMEI0787
Protein Accession	NP_539704.1
Other Database IDs	UniProtKB-ID: D0B951_BRUME UniRef100: UniRef100_B2S648 UniRef90: UniRef90_B2S648 UniRef50: UniRef50_B2S648 UniParc: UPI00001334D4 EMBL: GG703780 EMBL-CDS: EEW86707.1 RefSeq_NT: NC_003317.1 OMA: GRDNTIT
Taxonomy ID	224914
Chromosome No	I
Gene Starting Position	806715
Gene Ending Position	807851
Gene Strand (Orientation)	+
Protein Name	recombinase A
DNA Sequence	>gi\|17986284:806715-807851 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence TTTGTGGCAACGAGCCTGCCGGGCTTCTAGCGAGACAAGAGGATGGTGTAAGATGTCTCAAAATTCATTG CGACTTGTTGAGGATAATTCAGTGGACAAGACAAAAGCTCTCGACGCGGCATTGTCGCAAATCGAACGGG CGTTCGGCAAAGGCTCAATCATGCGTCTGGGCCAGAACGATCAGGTGGTCGAAATCGAAACCGTATCGAC CGGCTCGCTCTCGCTCGATATTGCCTTGGGGGTTGGCGGCTTGCCCAAGGGACGTATCGTGGAAATCTAT GGGCCGGAAAGTTCCGGTAAAACCACGCTTGCCTTGCACACCATCGCTGAAGCGCAGAAGAAGGGCGGCA TCTGCGCATTCGTCGATGCGGAACATGCGCTTGACCCGGTCTATGCGCGCAAGCTCGGTGTCGATCTTGA AAATCTTCTGATCTCGCAGCCCGATACGGGTGAACAGGCGCTTGAAATCACCGATACGCTCGTGCGCTCT GGCGCCATCGACGTTCTCGTTGTCGACTCGGTCGCAGCCCTGACGCCGCGTGCTGAAATCGAAGGTGAAA TGGGGGATTCCCTGCCGGGCCTTCAGGCCCGTCTCATGAGCCAGGCGCTGCGCAAGCTTACTGGCTCCAT TTCCCGTTCAAACTGCATGGTGATCTTCATCAACCAGATCCGCATGAAGATCGGTGTCATGTTCGGCTCG CCGGAAACGACAACGGGCGGCAATGCGCTCAAGTTCTATGCTTCGGTTCGCCTCGATATTCGCCGTATCG GCTCCATCAAGGAGCGCGACGAAGTGGTGGGCAACCAGACCCGCGTGAAGGTGGTGAAGAACAAGCTCGC TCCTCCGTTCAAGCAGGTCGAATTCGATATCATGTATGGCGCGGGCGTTTCCAAGGTGGGCGAGCTGGTC GATCTTGGTGTCAAGGCCGGTGTGGTTGAGAAGTCTGGTGCCTGGTTCTCCTATAATTCCCAGCGTCTCG GGCAGGGGCGCGAGAACGCCAAGCAATATCTCAAGGATAATCCGGAAGTCGCGCGTGAAATCGAAACCAC GCTTCGCCAGAATGCCGGCCTGATCGCCGAGCAGTTCCTTGACGATGGCGGACCGGAAGAAGATGCTGCC GGCGCGGCGGAAATGTA
Protein Sequence	>gi\|229220681\|ref\|NP_539704.2\| recombinase A [Brucella melitensis bv. 1 str. 16M] MSQNSLRLVEDNSVDKTKALDAALSQIERAFGKGSIMRLGQNDQVVEIETVSTGSLSLDIALGVGGLPKGRIVEIYGPESSGKTTLALHTIAEAQKKGGICAFVDAEHALDPVYARKLGVDLENLLISQPDTGEQALEITDTLVRSGAIDVLVVDSVAALTPRAEIEGEMGDSLPGLQARLMSQALRKLTGSISRSNCMVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGSIKERDEVVGNQTRVKVVKNKLAPPFKQVEFDIMYGAGVSKVGELVDLGVKAGVVEKSGAWFSYNSQRLGQGRENAKQYLKDNPEVAREIETTLRQNAGLIAEQFLDDGGPEEDAAGAAEM
Molecule Role	Virulence factor
Molecule Role Annotation	FUNCTION: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage (By similarity)(UniProt: P65976). SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: P65976). SIMILARITY: Belongs to the recA family(UniProt: P65976). MUTATION: The RecA mutant was more sensitive than the parental strain to killing by MMS. When administered intraperitoneally to BALBc mice, numbers of bacteria per spleen were consistently lower in animals infected with the RecA mutant than with the parental strain. However, both the RecA mutant and parental strain persisted in mice through 100 days post-infection. These results indicate that RecA is not crucial for persistence of B abortus in mice (Tatum et al., 1993). The B abortus RecA mutant was virulent in mice, but its course of infection in mice differed from that of the parental strain. The infectious cycle of the parental strain in the mouse model was biphasic. During the rst week, there was an initial rise in cfu of B abortus 2308 in the spleen followed by a decrease during the second week. This phase was followed by a second phase in which B abortus S2308 persisted and slowly increased in numbers in the spleen . Though fewer RecA mutants were found in the spleens of mice infected intraperitoneally in the early stages of the infection and no large initial rise was seen, the same numbers were found as the parental strain 100 days post -infection. This suggests that collectively, different loci are involved to varying extents in the initial infection and the persistence phase (Tatum et al., 1993).
References	*Tatum et al., 1993:* Tatum FM, Morfitt DC, Halling SM. Construction of a Brucella abortus RecA mutant and its survival in mice. Microbial pathogenesis. 1993; 14(3); 177-185. [PubMed: 8321120].


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recA from Brucella melitensis bv. 1 str. 16M