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rpoA from Brucella melitensis bv. 1 str. 16M

Victor ID 1244
Gene Name rpoA from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1196492
NCBI Protein GI 17987064
Locus Tag BMEI0781
Protein Accession NP_539698.1
Other Database IDs UniProtKB-ID: D0B945_BRUME
UniRef100: UniRef100_C0RJH6
UniRef90: UniRef90_B2S655
UniRef50: UniRef50_Q5HAU5
UniParc: UPI0000057DF7
EMBL: GG703780
EMBL-CDS: EEW86701.1
RefSeq_NT: NC_003317.1
OMA: FGTTLGN
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 800211
Gene Ending Position 801224
Gene Strand (Orientation) +
Protein Name DNA-directed RNA polymerase subunit alpha
DNA Sequence 
>gi|17986284:800211-801224 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
CATGATCCAGAAGAACTGGCAGGAACTGATCAAGCCGAACAAGGTGGATTTCATCACCCACGGCTCCCGC
ACGCATGCAACCGTCGTCGCTGAGCCGCTGGAACGCGGCTTTGGCCTGACGCTCGGCAATGCGCTGCGTC
GCGTGCTTCTGTCGTCGCTTCGCGGCGCTGCCGTGACCGCTGTCCAGATCGACGGCGTTCTGCATGAATT
CTCTTCCATTCCGGGCGTTCGCGAAGACGTCACGGATATCGTTCTGAACATCAAGGAAATCGCCATCCGT
ATGGAAGGCGAGGGCCCGAAGCGCATGGTCGTCCACAAGGAAGGCCCCGGCGTCGTTACGGCTGGCGACA
TTCAGACCGTTGGCGATGTCGAGATCCTGAACCCGGAACACGTCATCTGCACGCTGGATGAAGGCGCTGA
AATCCGCATGGAATTCACCGTCAACACCGGCAAGGGATATGTGCCTGCCGACTGCAACCGCGCGGAAGAT
GCTCCGATCGGTCTGATCCCGGTCGATAGCCTTTATTCTCCGGTTCGCAAGGTGTCGTACAAGATCGAGA
ACACCCGTGAAGGCCAGGTTCTCGACTATGACAAGCTGACGCTGAATATCGAGACCAATGGTTCGGTTAC
GGGTGAAGACGCTGTGGCTTATGCCGCTCGCATTCTCCAGGACCAGCTTTCGATCTTCGTCAACTTTGAA
GAGCCGCAGAAGGAAGCTCCGCAGGAACAGGTTGCGGAACTGGCCTTCAATCCGGCTCTGCTCAAGAAGG
TGGACGAACTGGAGCTGTCGGTGCGTTCGGCAAACTGCCTGAAGAACGACAACATCGTCTATATCGGCGA
TCTGATCCAGAAGACGGAAGCCGAAATGCTGCGGACCCCGAATTTCGGCCGCAAGTCGCTTAACGAGATC
AAGGAAGTTCTGGCATCGATGGGTCTCCATCTCGGTATGGAAATCCCAGCCTGGCCGCCGGAAAATATCG
AAGACCTCGCCAAGCGCTACGAAGACCAATATTG
Protein Sequence 
>gi|17987064|ref|NP_539698.1| DNA-directed RNA polymerase subunit alpha [Brucella melitensis bv. 1 str. 16M] MIQKNWQELIKPNKVDFITHGSRTHATVVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAVQIDGVLHEFSSIPGVREDVTDIVLNIKEIAIRMEGEGPKRMVVHKEGPGVVTAGDIQTVGDVEILNPEHVICTLDEGAEIRMEFTVNTGKGYVPADCNRAEDAPIGLIPVDSLYSPVRKVSYKIENTREGQVLDYDKLTLNIETNGSVTGEDAVAYAARILQDQLSIFVNFEEPQKEAPQEQVAELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEIPAWPPENIEDLAKRYEDQY
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates(UniProt: Q8G094).

CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)(UniProt: Q8G094).

SUBUNIT: Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity)(UniProt: Q8G094).

DOMAIN: The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements (By similarity)(UniProt: Q8G094).

SIMILARITY: Belongs to the RNA polymerase alpha chain family(UniProt: Q8G094).

MUTATION: The rpoA gene codes for the essential alpha-subunit of the RNA polymerase. B. melitensis rpoA mutant was found by signature-tagged mutagenesis from a mouse infection model. This disruption leaves a partially functional protein, impaired for the activation of virB transcription, as demonstrated by the absence of induction of the virB promoter in the Tn5::rpoA background. RpoA is involved in virB regulation in vitro. The mutant (Tn5::rpoA) was more resistant to oxidative stress (Lestrate et al., 2003).
COG COG0202K, under K: Transcription
References
Lestrate et al., 2003: Lestrate P, Dricot A, Delrue RM, Lambert C, Martinelli V, De Bolle X, Letesson JJ, Tibor A. Attenuated signature-tagged mutagenesis mutants of Brucella melitensis identified during the acute phase of infection in mice. Infection and immunity. 2003; 71(12); 7053-7060. [PubMed: 14638795].