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cobB from Brucella melitensis bv. 1 str. 16M

Victor ID 1241
Gene Name cobB from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1196416
NCBI Protein GI 17986988
Locus Tag BMEI0705
Protein Accession NP_539622.1
Other Database IDs UniProtKB-ID: D0B8X0_BRUME
UniRef100: UniRef100_Q57CK2
UniRef90: UniRef90_A5VR62
UniRef50: UniRef50_A6X051
UniParc: UPI0000057DAB
EMBL: GG703780
EMBL-CDS: EEW86626.1
RefSeq_NT: NC_003317.1
OMA: VFKCGPD
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 730440
Gene Ending Position 731750
Gene Strand (Orientation) +
Protein Name cobyrinic acid a,c-diamide synthase
DNA Sequence 
>gi|17986284:730440-731750 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
CATGAAGGGATTCATGATTGCCGCACCGGCCTCCGGGTCGGGCAAGACGACCGTGACCTTGGGTTTGTTG
CGCGCCCTGAAACGCAGGGGCGAGGTGCTTGCGCCGGTGAAGGCCGGGCCGGATTATATCGACCCCGCCT
ATCACAGGGCGGCGAGTGGCGTGGATTGTTTCAATCTCGACCCCTGGGCCATGCGACCGGAACTCATCAG
TGCGCTTTCCTCCCGCATGACGGAAAGCGGCGCGCGTGTTCTTGTCGCCGAAGGCATGATGGGGCTTTTC
GACGGGGCGATTGACGGTAAGGGCTCTTCTGCCGATCTGGCGCGGCTTCTGGATTTGCCAGTCGTGCTGG
TGGTGGATTGCGCCCGGCAATCCCACTCCATCGCGGCGCTGGTGTGGGGTTTCAGCCAGTTTCGCAAGGA
TGTGCTGATTGAGGGTGTCATTCTCAATCGTGTCGGAAGTCCCCGCCATGAAGCCATGCTGCGGGGCGCT
CTGGCACCCCTTGGCGTCCCGGTTCTGGGTGCGCTGCCGCGAGATCCGGCACTTTCCTTGCCGGAAAGGC
ATCTGGGGCTGGTGCAGGCGGACGAACATGCCGGTCTTGAAAGCTTTCTCGAACAAGCGGCCGATGTGAT
GGAAGCGCATATCGACATGGATGCGCTTCAGACCATCTGGCTACGCCCGAAGCGCTATGACGCCATGGCC
AATGTCGCGCGTCTGAAACCGCTTGGAAACCGCATTGCCGTGGCGCGGGACGATGCCTTTGCTTTTGCCT
ATATGCATCTTTTTGAAGGCTGGCGCAGGCGGGGTGCGGAAATCTCTTTCTTTTCCCCGCTGGCAGATGA
AGCACCGAAGGCAGATGCGGATGCAATCTATCTTCCCGGTGGTTATCCAGAACTTCATGCGCAACGATTG
GCCGGTGCATCCCGTTTCCGCACGGCGATAGGGGACGCTGCCGCGCGTGGCGTCACGGTCTATGGCGAGT
GCGGCGGCTATATGGTCCTCGGGAAAACACTGGAGGATGCGGCAGGCGTTCACCATCCCATGCTGGGGCT
TTTGCCATTGGAAACGAGCTTTGCCAGGCGGAAACTGCATCTTGGCTATCGCCTGCTGGAGCCGCTTGGC
GGATTGCCGTGGGACATGCCTTTGAAAGCGCATGAATTCCATTATGCAAGCATCGTGCGGGAAGAAAAGG
CCGACCGCCTGTTCCGGGTGCGCGATGCATCGGGCGAGAACCTTGGCGAAGCGGGCTTGCGGGTTGGTTC
CGTCTCCGGCTCCTTCATGCATGTCATTGATTTTAGCGGAGAAGCGGCGTG
Protein Sequence 
>gi|17986988|ref|NP_539622.1| cobyrinic acid a,c-diamide synthase [Brucella melitensis bv. 1 str. 16M] MKGFMIAAPASGSGKTTVTLGLLRALKRRGEVLAPVKAGPDYIDPAYHRAASGVDCFNLDPWAMRPELISALSSRMTESGARVLVAEGMMGLFDGAIDGKGSSADLARLLDLPVVLVVDCARQSHSIAALVWGFSQFRKDVLIEGVILNRVGSPRHEAMLRGALAPLGVPVLGALPRDPALSLPERHLGLVQADEHAGLESFLEQAADVMEAHIDMDALQTIWLRPKRYDAMANVARLKPLGNRIAVARDDAFAFAYMHLFEGWRRRGAEISFFSPLADEAPKADADAIYLPGGYPELHAQRLAGASRFRTAIGDAAARGVTVYGECGGYMVLGKTLEDAAGVHHPMLGLLPLETSFARRKLHLGYRLLEPLGGLPWDMPLKAHEFHYASIVREEKADRLFRVRDASGENLGEAGLRVGSVSGSFMHVIDFSGEAA
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation (By similarity)(UniProt: Q8G020).

PATHWAY: Cobalamin biosynthesis(UniProt: Q8G020).

SIMILARITY: Belongs to the cobB/cobQ family. CobB subfamily(UniProt: Q8G020).

MUTATION: 1,152 signature-tagged mutagenesis mutants of Brucella melitensis 16M were screened in a mouse model of infection. 36 of them to be attenuated in vivo. cobB is one of them (Lestrate et al., 2003).
COG COG1797H, under H: Coenzyme transport and metabolism
References
Lestrate et al., 2003: Lestrate P, Dricot A, Delrue RM, Lambert C, Martinelli V, De Bolle X, Letesson JJ, Tibor A. Attenuated signature-tagged mutagenesis mutants of Brucella melitensis identified during the acute phase of infection in mice. Infection and immunity. 2003; 71(12); 7053-7060. [PubMed: 14638795].