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ilvCv from Brucella melitensis bv. 1 str. 16M

Victor ID 1238
Gene Name ilvCv from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1196335
NCBI Protein GI 17986907
Locus Tag BMEI0624
Protein Accession NP_539541.1
Other Database IDs UniProtKB-ID: D0B8P2_BRUME
UniRef100: UniRef100_C0RE20
UniRef90: UniRef90_B2S6K5
UniRef50: UniRef50_A9M177
UniParc: UPI0000057D5A
EMBL: GG703780
EMBL-CDS: EEW86548.1
RefSeq_NT: NC_003317.1
OMA: DSISNTA
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 651727
Gene Ending Position 652746
Gene Strand (Orientation) +
Protein Name ketol-acid reductoisomerase
DNA Sequence 
>gi|17986284:651727-652746 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
AATGCGCGTTTATTACGATCGGGATGCAGACGTTAACCTGATCAAGTCGAAGAAGGTGGTTATCGTCGGT
TATGGCAGCCAGGGCCGCGCCCATGCGCTCAACCTGAAGGACTCTGGCGCGGCCAATGTGCGCGTTGCCC
TTCGTGAAGGCTCCGCCACGGTTCAGAAGGCACAGGCCGATGGCTTTGAAGTCATGAACGTCGCCGATGC
CGCCAAGTGGGCCGACCTCATGATGATGGCGACCCCTGACGAATTGCAGGCCGACATCTACCGCGACCAC
ATCCACAACAATCTGCGCGATGGCGCTGCGATTGCTTTTGCCCATGGCCTCAACGTGCATTGCGGCCTGA
TTGAGCCGAAGAAGACGGTCGATGTCATGATGATCGCGCCGAAGGGTCCCGGCCACACGGTTCGCGGTGA
ATATCAGAAGGGCGGCGGCGTTCCCTGCCTCATCGCCATTCATCAGGATGCATCTGGCAATGCCCATGAT
CTGGCCCTTTCCTACGCTTCGGGCGTTGGCGGCGGCCGTTCGGGCGTTATCGAGACGACCTTCAAGGAAG
AGTGCGAAACCGATCTCTTCGGCGAACAGGCTGTTCTGTGCGGCGGCGTGGTCGAGCTGATCCGCACCGG
CTTTGAAGTTCTGGTCGAAGCTGGCTATGCGCCGGAAATGGCCTATTTCGAGTGCCTGAACGAAATGAAG
CTGATCGTGGACCTGATCTACGAAGGCGGCATTGCCAACATGAACTATTCGATCTCCAACACTGCCGAGT
GGGGCGAATATGTGACCGGCCCGCGCATCATCACCGCCGAAACCAAGGCCGAAATGAAGCGTGTTCTGAA
GGATATCCAGACCGGCAAGTTCACCTCTGACTGGATGCAGGAATGGAAAGCCGGCGCTGCCCGCTTCAAG
GGTATCCGCCGTCTCAACGACGCCCATCAGATCGAAGAAGTCGGTGGCAAGCTACGCGCCATGATGCCGT
GGATCGAAAAGAACAAACTGGTCGATAAAGCCCGCAACTG
Protein Sequence 
>gi|17986907|ref|NP_539541.1| ketol-acid reductoisomerase [Brucella melitensis bv. 1 str. 16M] MRVYYDRDADVNLIKSKKVVIVGYGSQGRAHALNLKDSGAANVRVALREGSATVQKAQADGFEVMNVADAAKWADLMMMATPDELQADIYRDHIHNNLRDGAAIAFAHGLNVHCGLIEPKKTVDVMMIAPKGPGHTVRGEYQKGGGVPCLIAIHQDASGNAHDLALSYASGVGGGRSGVIETTFKEECETDLFGEQAVLCGGVVELIRTGFEVLVEAGYAPEMAYFECLNEMKLIVDLIYEGGIANMNYSISNTAEWGEYVTGPRIITAETKAEMKRVLKDIQTGKFTSDWMQEWKAGAARFKGIRRLNDAHQIEEVGGKLRAMMPWIEKNKLVDKARN
Molecule Role Virulence factor
Molecule Role Annotation CATALYTIC ACTIVITY: (R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH(UniProt: Q8FZU1).

CATALYTIC ACTIVITY: (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH(UniProt: Q8FZU1).

PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2(UniProt: Q8FZU1).

PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2(UniProt: Q8FZU1).

SIMILARITY: Belongs to the ketol-acid reductoisomerase family(UniProt: Q8FZU1).

MUTATION: ilvC is one of the 37 mutants with virulene defect, screened out from the signature- tagged miniTn5 library (Lestrate et al., 2003).
COG COG0059EH, under E: Amino acid transport and metabolism; H: Coenzyme transport and metabolism
References
Lestrate et al., 2003: Lestrate P, Dricot A, Delrue RM, Lambert C, Martinelli V, De Bolle X, Letesson JJ, Tibor A. Attenuated signature-tagged mutagenesis mutants of Brucella melitensis identified during the acute phase of infection in mice. Infection and immunity. 2003; 71(12); 7053-7060. [PubMed: 14638795].