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miaA from Brucella melitensis bv. 1 str. 16M

Victor ID 1236
Gene Name miaA from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1196327
NCBI Protein GI 17986899
Locus Tag BMEI0616
Protein Accession NP_539533.1
Other Database IDs UniProtKB-ID: D0B8N4_BRUME
UniRef100: UniRef100_Q8YI29
UniRef90: UniRef90_B2S6L5
UniRef50: UniRef50_A9M648
UniParc: UPI0000057D52
EMBL: GG703780
EMBL-CDS: EEW86540.1
RefSeq_NT: NC_003317.1
OMA: VNADSMQ
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 642158
Gene Ending Position 643090
Gene Strand (Orientation) +
Protein Name tRNA delta(2)-isopentenylpyrophosphate transferase
DNA Sequence 
>gi|17986284:642158-643090 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
GATGAGTGAGGATGCAGTCAAGAATGCGATCCTGATAGCTGGCCCAACGGCCAGCGGCAAGTCGGCACTT
GCCATTCGCATGGCCAAAGCGACGGGCGGTTTCATCGTCAATACCGATTCCATGCAGGTTTATGGCGTTC
TGGATCTTTTGACCGCGCGGCCTTCCAGGGCCAATCTGGCCGAGGCCGAACATTTTCTTTACGGCCATGT
GCCGCCCTCCAGCACCTATTCCACCGGCAAATGGTTTGAGGATGTCGAAGCACTTCTGGGGCGTTGCGAA
CTGCAAGGCCGGGTGCCGATCTTCGTCGGCGGGACAGGGCTTTATTTTCGTGCGCTTCTGGGCGGGCTTT
CGCAAACGCCGGAAGTATCGGCGCAAGTGCGCGACCATTGGCGAGGCCGGATGGAAGCCGAAGGCGCAAA
GGCCCTGCACGCTGTCCTTTGTGTGCGTGACCCGGCCATCGCGGCTGCCTTGCAGCCCTCCGATAGCCAG
CGCATTGTGCGTGCGCTGGAAGTGCTGGAAAGCACCGGCAAATCCCTTTTGGAATGGCAAAAGGTCAAGG
GCAGGGCGCTGGTGGATGACCAATCGGCGCAAAAGATCGTGCTCCGGCCTGATCGTGCATGGCTTGGCGT
GCGTATTGCGCGGCGTTTTTCGGCCATGTGGGCGGAGGGGGCAATCGATGAGGTGAGGGCGCTTCTGGCG
CTCGATCTCGATCCTGCGCTACCCGCCATGAAGGCCATTGGCGTCAGGGAAGTCAGTGCATTTCTTGCAG
AAACCATGTCGCGCGAGGAGGCTATTGAACGTTCCGTCATTGCCACGCGGCAATATGCCAAGCGCCAGTC
CACCTGGTTTCGCAATCAGTTGGGCGAAGACTGGCGTGTCTATGCCAGTGGCGAAGAGGTGTTTCAGGGC
GGTTCGTTTCGGGATCCGCAATA
Protein Sequence 
>gi|17986899|ref|NP_539533.1| tRNA delta(2)-isopentenylpyrophosphate transferase [Brucella melitensis bv. 1 str. 16M] MSEDAVKNAILIAGPTASGKSALAIRMAKATGGFIVNTDSMQVYGVLDLLTARPSRANLAEAEHFLYGHVPPSSTYSTGKWFEDVEALLGRCELQGRVPIFVGGTGLYFRALLGGLSQTPEVSAQVRDHWRGRMEAEGAKALHAVLCVRDPAIAAALQPSDSQRIVRALEVLESTGKSLLEWQKVKGRALVDDQSAQKIVLRPDRAWLGVRIARRFSAMWAEGAIDEVRALLALDLDPALPAMKAIGVREVSAFLAETMSREEAIERSVIATRQYAKRQSTWFRNQLGEDWRVYASGEEVFQGGSFRDPQ
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Catalyzes the first step in the biosynthesis of 2-methylthio-N6-(delta(2)-isopentenyl)-adenosine (MS[2]I[6]A) adjacent to the anticodon of several tRNA species (By similarity)(UniProt: Q8CY40).

CATALYTIC ACTIVITY: Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine(UniProt: Q8CY40).

SIMILARITY: Belongs to the IPP transferase family(UniProt: Q8CY40).

MUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis miaA gene is required for intracellular multiplication in human macrophage THP-1 cells (Kohler et al., 2002).
COG COG0324J, under J: Translation, ribosomal structure and biogenesis
References
Kohler et al., 2002: Kohler S, Foulongne V, Ouahrani-Bettache S, Bourg G, Teyssier J, Ramuz M, Liautard JP. The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. Proceedings of the National Academy of Sciences of the United States of America. 2002 Nov 26; 99(24); 15711-6. [PubMed: 12438693 ].