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pncA from Brucella melitensis bv. 1 str. 16M

Victor ID 1233
Gene Name pncA from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1196256
NCBI Protein GI 17986828
Locus Tag BMEI0545
Protein Accession NP_539462.1
Other Database IDs UniProtKB-ID: Q8YIA0_BRUME
UniRef100: UniRef100_Q8YIA0
UniRef90: UniRef90_E0DY99
UniRef50: UniRef50_C3KLM5
UniParc: UPI0000057D0B
EMBL: AE008917
EMBL-CDS: AAL51726.1
RefSeq_NT: NC_003317.1
GenomeReviews: AE008917_GR
KEGG: bme:BMEI0545
NMPDR: fig|224914.1.peg.544
HOGENOM: HBG289389
OMA: LYLEVID
ProtClustDB: PRK14013
BioCyc: BMEL224914:BMEI0545-MONOMER
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 564907
Gene Ending Position 565968
Gene Strand (Orientation) +
Protein Name INTEGRAL MEMBRANE PROTEIN
DNA Sequence 
>gi|17986284:564907-565968 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
GATGGCCGCCTATCGTTATTTTATTTGGCCCATGATTTTCACCGTTTTGGGTTTGGCCGGTGCCGTATGG
CTCGGTTACGAACTGACCGGCACTTTTGGGGGAATGGTTTCCGTTTTCATCATTTGTGCGGTTTTGAGCG
TTCTCGAAATCTCGCTTTCCTTCGATAATGCCATCGTCAATGCGCGTATCCTGCGCGATATGTCACCGAA
ATGGCAGCACCGCTTCCTGACATGGGGCATCGTCATCGCCGTTTTCGGCATGCGCATCGTCTTCCCGCTG
GGAATCGTGGCCATAGCCATGTGGACGGACCCGATCTCGGCGCTGAAACTTGCCATCTGGCAGCCCAATG
AATATGCGCGCGTGATTGCGGAATCCCATACGGGCATCGCCGCGTTCGGCGGCACGTTCCTTCTGATGGT
CGGCATGAAATATTTCTTCGACGTTGAAAAGGATGTGCACTGGATCAGGATGATCGAGAGCCGCATGTCG
AAATTCGCATCCGTTCAGGGTGTTGAAATCGGCGTCGCCATTGCCCTTATCATCTTCTTCTCCTACCAGC
TTGACGCCGACCATTCGCATACATTCGTTATTGCGGGGCTGTTTGGTCTTCTGACCTTCCTTGCAGTGGA
AGGGCTTGGCGAAGTTCTGGATGCGACGCAGGAGCAGATGGATATGGTCCATCGCGGCGGTCTTGGTGCG
TTTATCTATCTGGAAGTGCTGGATGCCAGCTTTTCCTTTGACGGCGTGATCGGTGCTTTCGCACTCACCA
CGAACCTTTTCGTGATCGCCATCGGCCTTGGTATCGGCGCGTTTTATGTGCGCTCGCTCACGATCATGCT
GGTGGAGCGCAAGACGCTTGGCCAGTACCGGTATCTGGAGCACGGTGCGTTTTACGCTATCCTCGTGCTG
GCGATCATCATGTATGTACAGACGCTGGTGCATATACCGGAAGTTATTACCGGCCTCATCGGCGCGGCAC
TTATCGGCCTCTCCTTCATGTCGTCGATGCGCTATAACCGCCTGAACCCGAACTGGCAGAACGAGACCGA
GGAAGTGACCTA
Protein Sequence 
>gi|17986828|ref|NP_539462.1| hypothetical protein BMEI0545 [Brucella melitensis bv. 1 str. 16M] MAAYRYFIWPMIFTVLGLAGAVWLGYELTGTFGGMVSVFIICAVLSVLEISLSFDNAIVNARILRDMSPKWQHRFLTWGIVIAVFGMRIVFPLGIVAIAMWTDPISALKLAIWQPNEYARVIAESHTGIAAFGGTFLLMVGMKYFFDVEKDVHWIRMIESRMSKFASVQGVEIGVAIALIIFFSYQLDADHSHTFVIAGLFGLLTFLAVEGLGEVLDATQEQMDMVHRGGLGAFIYLEVLDASFSFDGVIGAFALTTNLFVIAIGLGIGAFYVRSLTIMLVERKTLGQYRYLEHGAFYAILVLAIIMYVQTLVHIPEVITGLIGAALIGLSFMSSMRYNRLNPNWQNETEEVT
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: Brucella abortus pnc mutant via mini-Tn5Km2 transposon mutagenesis has intracellular growth defect inside HeLa cells (Kim et al., 2003). Nicotinamidasepyrazinamidase mutant (pncA mutant) of Brucella abortus failed to replicate in HeLa cells, and showed a lower rate of intracellular replication than that of wild-type strain in macrophages (Kim et al., 2004). The pncA mutant was not co-localizing with either late endosomes or lysosomes. The pncA mutant showed a 1.5-log reduction of the number of bacteria isolated from mouse spleens after 10 weeks (Kim et al., 2004). It indicates that the mutant has reduced virulence in mice.
COG COG2899S, under S: Function unknown
References
Kim et al., 2003: Kim S, Watarai M, Kondo Y, Erdenebaatar J, Makino S, Shirahata T. Isolation and characterization of mini-Tn5Km2 insertion mutants of Brucella abortus deficient in internalization and intracellular growth in HeLa cells. Infection and immunity. 2003; 71(6); 3020-3027. [PubMed: 12761078].
Kim et al., 2004: Kim S, Kurokawa D, Watanabe K, Makino S, Shirahata T, Watarai M. Brucella abortus nicotinamidase (PncA) contributes to its intracellular replication and infectivity in mice. FEMS microbiology letters. 2004; 234(2); 289-295. [PubMed: 15135535].