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pth from Brucella melitensis bv. 1 str. 16M

Victor ID 1227
Gene Name pth from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1196191
NCBI Protein GI 17986763
Locus Tag BMEI0480
Protein Accession NP_539397.1
Other Database IDs UniProtKB-ID: D0B9P8_BRUME
UniRef100: UniRef100_A9M6K1
UniRef90: UniRef90_Q2YLX5
UniRef50: UniRef50_Q2YLX5
UniParc: UPI0000057CCB
EMBL: GG703780
EMBL-CDS: EEW86404.1
RefSeq_NT: NC_003317.1
OMA: IKFKTGG
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 501204
Gene Ending Position 501956
Gene Strand (Orientation) -
Protein Name peptidyl-tRNA hydrolase
DNA Sequence 
>gi|17986284:501204-501956 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
ATTAATCCTTCTTCCCCAGCAGCTTTTTCAGCATTTCAGCCATCGGGCCGCTTTCGGGAATATTGGGCTT
TTTCTGGTTCTGGCGCGCCTGACGAATATGGCTCTGGGCCTTTGGCGGAGCCTTCTCCAATTGCGCGGGA
TCTGTCTTGACGCCGCCTGGCCGCTGATTGCCGTCGCCCATGGCGAGTGCAATGCGGTTCATGAAGCTGT
TATCCTCGCGTCTGGCAAGCATGGCGACATTGTCGGCGACGGCGCCCATAAGCGTATCCAGCCATTCATT
GTCGGCCTTGGCGAAATCGCCCAGAACATAATTATGCACGAGTTCCTTCGCGCCGGGATGGCCGATGCCA
AGCCGCATGCGGCGATAATTCTGTCCGCCGGGGAAGGACTGCATATGTGCGTCGATGGATTTGATGCCGT
TATGGCCGCCGGAACCGCCGCCGGTCTTGATGCGCAGCTTGCCCGGAACGAGGTCCAGTTCATCATAGAT
CACGACGAGATCGGCAGGCGTCAGCTTGTAGAAGCGCATGGCCTCGCCGATAGACTGGCCGGAAAGGTTC
ATGAAAGTCTGCGGTTTGACCAGCAACACTTTCTCGCCGTCGATCACGCCATCGGCGATCTCTGCCTGAA
ATTTCTTCTGCCAGTTGGAAAAGCGATGGCGGCGGAATATTTCATCCGCCGCCATGAAACCGATATTGTG
CCGGTTATGCGCATATTGGGGACCCGGATTGCCAAGACCTGCGATGAGCAGCA
Protein Sequence 
>gi|17986763|ref|NP_539397.1| peptidyl-tRNA hydrolase [Brucella melitensis bv. 1 str. 16M] MLLIAGLGNPGPQYAHNRHNIGFMAADEIFRRHRFSNWQKKFQAEIADGVIDGEKVLLVKPQTFMNLSGQSIGEAMRFYKLTPADLVVIYDELDLVPGKLRIKTGGGSGGHNGIKSIDAHMQSFPGGQNYRRMRLGIGHPGAKELVHNYVLGDFAKADNEWLDTLMGAVADNVAMLARREDNSFMNRIALAMGDGNQRPGGVKTDPAQLEKAPPKAQSHIRQARQNQKKPNIPESGPMAEMLKKLLGKKD
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis (By similarity)(UniProt: P65864).

CATALYTIC ACTIVITY: N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA(UniProt: P65864).

SUBUNIT: Monomer (By similarity)(UniProt: P65864).

SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: P65864).

SIMILARITY: Belongs to the PTH family(UniProt: P65864).

MUTATION: pth encodes for a peptidyl tRNA hydrolase. Transposon insertion in pth has a polar effect on dugA expression and that the pth/dugA mutant is deficient in iron assimilation because of altered expression of the dugA gene. Only minor difference in intracellular growth in bovine macrophages and HeLa cells between the pth/dugA mutant and wild-type strains was observed (Danese et al., 2004).
COG COG0193J, under J: Translation, ribosomal structure and biogenesis
References
Danese et al., 2004: Danese I, Haine V, Delrue RM, Tibor A, Lestrate P, Stevaux O, Mertens P, Paquet JY, Godfroid J, De Bolle X, Letesson JJ. The Ton system, an ABC transporter, and a universally conserved GTPase are involved in iron utilization by Brucella melitensis 16M. Infection and immunity. 2004; 72(10); 5783-5790. [PubMed: 15385478].