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leuA from Brucella melitensis bv. 1 str. 16M

Victor ID 1225
Gene Name leuA from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1196162
NCBI Protein GI 17986734
Locus Tag BMEI0451
Protein Accession NP_539368.1
Other Database IDs UniProtKB-ID: LEU1_BRUME
UniRef100: UniRef100_Q8YIJ3
UniRef90: UniRef90_Q8FZC4
UniRef50: UniRef50_A4QAP0
UniParc: UPI000012E4B1
EMBL: AE008917
EMBL-CDS: AAL51632.1
RefSeq_NT: NC_003317.1
GenomeReviews: AE008917_GR
KEGG: bme:BMEI0451
NMPDR: fig|224914.1.peg.450
HOGENOM: HBG289752
OMA: VERCNQI
ProtClustDB: PRK03739
BioCyc: BMEL224914:BMEI0451-MONOMER
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 470496
Gene Ending Position 472247
Gene Strand (Orientation) +
Protein Name 2-isopropylmalate synthase
DNA Sequence 
>gi|17986284:470496-472247 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
GTTGAAAGCCGGGAGGAAACGAGAAATGAACCAGCCTGTCTCCACGCCAAAGTCCGTGTCCCAAGCCGTA
TCGGCACGCAAGGGCATGCCTATTGCCGGTACCAAATACGCGCCGTTCCCTGCGCCGCAGCTCGATGACC
GCACTTGGCCTTCCAAACGCATCGAAAAGGCGCCGATCTGGTGTTCGGTCGATCTGCGCGACGGCAACCA
GGCTTTGATCGACCCCATGGGGCATGACCGCAAGGAGCGCATGTTCCGCCTGCTGATCGATATGGGTTTC
CCGGAAATCGAGATCGGTTTCCCGTCCGCTTCGCAGACGGATTTCGACTTCTGCCGCTGGGCCATTGAAC
AGGGCGATGTTCCTGACGACGTGGATTTGCAGGTTCTGGTCCAGTGCCGTCCTGAATTGATTACGCGCAC
TTTCGAGGCGCTGGAAGGCGCGAAGACGCCGATCATCCATTTCTACAATTCCACCAGCGAATTGCAGCGC
CGCGTGGTGTTTGCCAAGGATGTCGGCGGCATCAAGCAGATTGCAACCGATGCGGCCAAGATGATCATGG
ATATGGCTGCCAAGGCTGGCGGCGGTTATCGCTTCCAGTATTCGCCGGAAAGCTTTACCGGCACCGAACT
GGATGTGGCGCTGGAAATCTGCAATGCGGTGATCGAGATCGTGAAGCCGACGCCGGACAACAAGCTGATC
GTCAACCTGCCTTCGACGGTGGAGATGAACACGCCGAATGTCTATGCCGACCAGATCGAATGGATGTGCC
GCAATCTGGACAATCGCGAGAGCCTGATTATCTCGCTGCATCCGCATAATGACCGCGGCACGGGCATTGC
CGCCACTGAACTGGGCCTGATGGCAGGCGCTGATCGCGGGGAAGGCACGCTGTTCGGCAATGGCGAGCGC
ACGGGCAATGTGGATGTGGTGACGCTGGCGCTCAACATGTACACGCAGGGCATCGACCCCGGGCTGGATT
GCACCGATATCAACCGGATGAAGGAAGTCTACGAATATTCCAACCAGTTGAAGATCGCCGAGCGCCATCC
TTATGTGGGCGAGCTGGTCTATACGGCTTTCTCCGGCTCGCATCAGGATGCGATCAACAAGGGCATGAAA
GCGCGCCGTTCGGCCAATTCGCCGGTTTGGGAAGTTCCTTACCTGCCGATCGATCCGCAGGATGTGGGCC
GCTCCTACGAGGCGATCATCCGCATCAATTCGCAATCGGGCAAGGGCGGTATCGCCTATATCCTGCAAGC
CGATTACGGCCTGAACCTGCCGCGCAACCTGCAGGTCGAGTTCCGCGAAATCATCCAGCACATTACGGAT
GAAGAGGGCAAGGAGCTGCCATCGAAGCGCATTTACGAGGAGTTCCAGAAACTCTATGTGACGCAGCCCG
ATGCCCGCATCAAGTTCGTGGATCACCACACCTATCCCCATCCTGAGCAAAAGGGCCGGCGCATCCTGAC
GGCTGAGATCACCGATAACGGTGTCACCAAGACTATCGAGGGCAAGGGCACGGGACCGATCGATGGTTTC
GTGGATGCGCTGTCGAAATATCTTGGCGTGAAAATGTCGGTGGTCGATTATTCCGAACATTCGCTCCAGC
AGGGTTCGGATGCTTCGGCCATTTCCTATGTCGAGATGGTTTATCCCGGCGGCAAGCTGTTCGGTGTCGG
CATCAACGATAATATCGTCAGCGCCTCGCTTGAAGCCGTGGTGTCGGCGGCAAATCGTGTGATTGCGAAG
TA
Protein Sequence 
>gi|161511158|ref|NP_539368.2| 2-isopropylmalate synthase [Brucella melitensis bv. 1 str. 16M] MPIAGTKYAPFPAPQLDDRTWPSKRIEKAPIWCSVDLRDGNQALIDPMGHDRKERMFRLLIDMGFPEIEIGFPSASQTDFDFCRWAIEQGDVPDDVDLQVLVQCRPELITRTFEALEGAKTPIIHFYNSTSELQRRVVFAKDVGGIKQIATDAAKMIMDMAAKAGGGYRFQYSPESFTGTELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVYADQIEWMCRNLDNRESLIISLHPHNDRGTGIAATELGLMAGADRGEGTLFGNGERTGNVDVVTLALNMYTQGIDPGLDCTDINRMKEVYEYSNQLKIAERHPYVGELVYTAFSGSHQDAINKGMKARRSANSPVWEVPYLPIDPQDVGRSYEAIIRINSQSGKGGIAYILQADYGLNLPRNLQVEFREIIQHITDEEGKELPSKRIYEEFQKLYVTQPDARIKFVDHHTYPHPEQKGRRILTAEITDNGVTKTIEGKGTGPIDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVEMVYPGGKLFGVGINDNIVSASLEAVVSAANRVIAK
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)(UniProt: Q8FZC4).

CATALYTIC ACTIVITY: Acetyl-CoA + 3-methyl-2-oxobutanoate + H(2)O = (2S)-2-isopropylmalate + CoA(UniProt: Q8FZC4).

PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1(UniProt: Q8FZC4).

SUBUNIT: Homotetramer (By similarity)(UniProt: Q8FZC4).

SIMILARITY: Belongs to the alpha-IPM synthetase/homocitrate synthase family. LeuA type 2 subfamily(UniProt: Q8FZC4).

MUTATION: leuA is a B. suis gene identified by signature-tagged mutagenesis. It is attenuated inside THP1 macrophage cell line.
References