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dut from Brucella melitensis bv. 1 str. 16M

Victor ID 1219
Gene Name dut from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1196069
NCBI Protein GI 17986641
Locus Tag BMEI0358
Protein Accession NP_539275.1
Other Database IDs UniProtKB-ID: DUT_BRUME
UniRef100: UniRef100_B2S7B5
UniRef90: UniRef90_B2S7B5
UniRef50: UniRef50_B2S7B5
UniParc: UPI000012996B
EMBL: AE008917
EMBL-CDS: AAL51539.1
RefSeq_NT: NC_003317.1
GenomeReviews: AE008917_GR
KEGG: bme:BMEI0358
HOGENOM: HBG436079
OMA: LDLRACI
ProtClustDB: PRK00601
BioCyc: BMEL224914:BMEI0358-MONOMER
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 369589
Gene Ending Position 370113
Gene Strand (Orientation) +
Protein Name deoxyuridine 5'-triphosphate nucleotidohydrolase
DNA Sequence 
>gi|17986284:369589-370113 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
CTTGAAACACCACCTCGCAGGCCAACTGAATTTTTGCAGGAAAGGCTCCCTCATGACCGCAGCATCCTCT
TCCGCGCCAACGCTCGGCATCATTCGCCTCGAACATGCGAAGGGCCTTGATCTTCCCGCCTATGAAACCG
CCGGTTCGGCGGGCATGGACCTGCGCGCCGCCGTGGCTGAAGATCGCCAGATCGTGCTCTTGCCCGGCCG
CCGCACGCTGGTGCCAACCGGCCTTATCCTTGAAATCCCGCAAGGCTATGAAGTACAGATCCGCCCCCGC
TCGGGCCTTGCCTTCAAGAATGGCATAACCTGCCTCAACACGCCCGGCACGATCGATTCGGATTATCGCG
GCGAAGTAAAAGTCCTGCTGATCAATCTTGGAGATGACGATTTCCGCATCGAGCGCGGAATGCGCATCGC
ACAGGCCGTGTTCGCTCCGGTCATCCAGCCGAAAATCGAGGAACGCGCCAAGATAAGTGAAACAGCCCGC
GGCGCGGGCGGGTTCGGTTCCACCGGCACAGCCTG
Protein Sequence 
>gi|17986641|ref|NP_539275.1| deoxyuridine 5'-triphosphate nucleotidohydrolase [Brucella melitensis bv. 1 str. 16M] MKHHLAGQLNFCRKGSLMTAASSSAPTLGIIRLEHAKGLDLPAYETAGSAGMDLRAAVAEDRQIVLLPGRRTLVPTGLILEIPQGYEVQIRPRSGLAFKNGITCLNTPGTIDSDYRGEVKVLLINLGDDDFRIERGMRIAQAVFAPVIQPKIEERAKISETARGAGGFGSTGTA
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA (By similarity)(UniProt: P64005).

CATALYTIC ACTIVITY: dUTP + H(2)O = dUMP + diphosphate(UniProt: P64005).

PATHWAY: De novo synthesis of thymidylate(UniProt: P64005).

SIMILARITY: Belongs toMUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis dut gene is required for intracellular multiplication in human macrophage THP-1 cells (Kohler et al., 2002). the dUTPase family(UniProt: P64005).
COG COG0756F, under F: Nucleotide transport and metabolism
References
Kohler et al., 2002: Kohler S, Foulongne V, Ouahrani-Bettache S, Bourg G, Teyssier J, Ramuz M, Liautard JP. The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. Proceedings of the National Academy of Sciences of the United States of America. 2002 Nov 26; 99(24); 15711-6. [PubMed: 12438693 ].