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purH from Brucella melitensis bv. 1 str. 16M

Victor ID 1210
Gene Name purH from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1195945
NCBI Protein GI 17986517
Locus Tag BMEI0233
Protein Accession NP_539151.1
Other Database IDs UniProtKB-ID: D0B9Y7_BRUME
UniRef100: UniRef100_A9M854
UniRef90: UniRef90_B2S7P0
UniRef50: UniRef50_B3DRY6
UniParc: UPI0000057BD6
EMBL: GG703781
EMBL-CDS: EEW86163.1
RefSeq_NT: NC_003317.1
OMA: VVKHVKS
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 240867
Gene Ending Position 242483
Gene Strand (Orientation) -
Protein Name bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
DNA Sequence 
>gi|17986284:240867-242483 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
ATCAGTGGCGGAAGTGGCGCATCCCCGTCATGACCATGGCGATGCCATGTTCATCGGCAGCGGCGATCAC
TTCGTCGTCACGCATGGAGCCGCCCGGCTGGATGACGGCGGTTGCACCGGCCTCGACAGCGGAAAGCAGA
CCGTCTGCAAAGGGGAAGAATGCATCGGACGCGACCACGCAGCCCTTGGTGAGCGGCGCTGCAAGGCCGG
CGGCTTCGGCCGCATCTTCTGCCTTGCGGGCGGCAATGCGGGCTGAATCCACGCGGCTCATCTGGCCTGC
GCCAATGCCGACCGTTGCGCCGTCCTTCACATAGACGATGGCGTTCGACTTCACATGCTTGCCGACGCGG
AAGGCGAATTTCAGATCGTTAAGCTCGGCTTCGGTCGGTGCGCGCTTGGTGACGACCTTGAGATCGAGAT
CGTCCACCACACCATTGTCGCGCGACTGGACCAGCAGGCCACCGGCAACCGTCTTGGCGGCAATGCCCTT
GGCGCGCGGGTCCGGCAGGCCGCCGGTGACAAGCAGGCGCAGGTTCTTCTTGGCCGCGACGATGGCCTGT
GCGCCTTCGGTGGCATCGGGCGCGATGATGACTTCTGTGAAGGTTTTCACGATCTCTTCAGCGGCTTCCT
CATCGAGCGTCCTGTTGAGCGCGACAATGCCGCCAAAGGCCGAAACCGGATCGCAGGCGAGTGCCTTGAG
GTAGGCTTCCTTAATGGTGGACGCTTCCGCCACGCCGCATGGATTGGCGTGCTTGATGATGGCAACGGCT
GCGGTGCGGGCGGGATCGAATTCGGCCACCAGCTCAAAAGCCGCATCGGTATCGTTGATGTTGTTATAGG
AAAGCTGCTTGCCCTGAAGCTGGGTCGCAGTGGCAACGCCGGGGCGCTTTTCGCCGGTGAGATAAAAGCC
AGCCGTCTGGTGCGGGTTTTCACCGTAGCGCATGACGGAATGCAGCTTGCCGGCAACCGCGCGATAGGTC
GGCGTTTCTTCGTCGATTGCTTCGGCAAACCAGTTGGAAATGGCCGCGTCATAAGCTGCCGTGCGCGAAA
AGGCCTTGGCGGCAAGCTTTTTGCGGAAGGCAAGCGGCAGGGAGCCGGAATGCTTTTCCAGTTCAGCCAC
GACGTCTGCATAATCGGCAGGGTCAACGACGGTTGCGACATAGGCGTGGTTCTTGGCCGAAGCGCGGATC
ATCGCCGGGCCGCCAATGTCGATATTCTCGACGGTCGTGTCGTAGTCGCCGCCCTTGAAGCGGACTTCCT
CGAACGGATAAAGATTGATGACGGCAAGATCGATGCCGCCAATGCCGTGTTCTTCCATGGCCGCGACATG
TTCCGGGTCGTTGCGCACGGCGAGCAGGCCGCCATGGACTGCCGGATGCAGCGTCTTGACGCGCCCATCC
ATGATTTCCGGAAAGCCGGTGATTTCGGATACGTCCTTCACCGGAATGCCTGCGGCGGCAATCGATTTTG
CTGTGCCGCCGGTCGAGAGGATTTCGACGCCATTGGCATGAAGCGCCTTGGCGAAATCGATCAGGCCGGT
TTTGTCGGAAACGGAAAGGAGGGCGCGGCGCACCCGATGAAGATCGGGAGCGGGAATATGCTTGGAGCTG
ACAGCCA
Protein Sequence 
>gi|17986517|ref|NP_539151.1| bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase [Brucella melitensis bv. 1 str. 16M] MAVSSKHIPAPDLHRVRRALLSVSDKTGLIDFAKALHANGVEILSTGGTAKSIAAAGIPVKDVSEITGFPEIMDGRVKTLHPAVHGGLLAVRNDPEHVAAMEEHGIGGIDLAVINLYPFEEVRFKGGDYDTTVENIDIGGPAMIRASAKNHAYVATVVDPADYADVVAELEKHSGSLPLAFRKKLAAKAFSRTAAYDAAISNWFAEAIDEETPTYRAVAGKLHSVMRYGENPHQTAGFYLTGEKRPGVATATQLQGKQLSYNNINDTDAAFELVAEFDPARTAAVAIIKHANPCGVAEASTIKEAYLKALACDPVSAFGGIVALNRTLDEEAAEEIVKTFTEVIIAPDATEGAQAIVAAKKNLRLLVTGGLPDPRAKGIAAKTVAGGLLVQSRDNGVVDDLDLKVVTKRAPTEAELNDLKFAFRVGKHVKSNAIVYVKDGATVGIGAGQMSRVDSARIAARKAEDAAEAAGLAAPLTKGCVVASDAFFPFADGLLSAVEAGATAVIQPGGSMRDDEVIAAADEHGIAMVMTGMRHFRH
Molecule Role Virulence factor
Molecule Role Annotation CATALYTIC ACTIVITY: 10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide(UniProt: P67540).

CATALYTIC ACTIVITY: IMP + H(2)O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide(UniProt: P67540).

PATHWAY: Nucleotide biosynthesis; IMP biosynthesis; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): single step(UniProt: P67540).

PATHWAY: Nucleotide biosynthesis; IMP biosynthesis; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: single step [final step](UniProt: P67540).

PATHWAY: Context: Purine biosynthesis(UniProt: P67540).

DOMAIN: The IMP cyclohydrolase activity resides in the N-terminal region (By similarity)(UniProt: P67540).

SIMILARITY: Belongs to the purH family(UniProt: P67540).

MUTATION: B. abortus mutant with mini-Tn5-disrupted purH displays nutritional defects in vitro (Alcantara et al., 2004).
COG COG0138F, under F: Nucleotide transport and metabolism
References
Alcantara et al., 2004: Alcantara RB, Read RD, Valderas MW, Brown TD, Roop RM 2nd. Intact purine biosynthesis pathways are required for wild-type virulence of Brucella abortus 2308 in the BALB/c mouse model. Infection and immunity. 2004; 72(8); 4911-4917. [PubMed: 15271960].