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leuC from Brucella melitensis bv. 1 str. 16M

Victor ID 1207
Gene Name leuC from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1195869
NCBI Protein GI 17986441
Locus Tag BMEI0157
Protein Accession NP_539075.1
Other Database IDs UniProtKB-ID: D0B3F7_BRUME
UniRef100: UniRef100_C0RFF1
UniRef90: UniRef90_B2S860
UniRef50: UniRef50_C1F700
UniParc: UPI0000057B8A
EMBL: GG703778
EMBL-CDS: EEW87931.1
RefSeq_NT: NC_003317.1
OMA: RPHAPKG
Taxonomy ID 224914
Chromosome No I
Gene Starting Position 151860
Gene Ending Position 153269
Gene Strand (Orientation) +
Protein Name isopropylmalate isomerase large subunit
DNA Sequence 
>gi|17986284:151860-153269 Brucella melitensis bv. 1 str. 16M chromosome chromosome I, complete sequence
AATGAGCGCGCCGCGTACACTTTATGACAAGATTTGGGATGACCATGTAGTGGATCAGCAGGAAGACGGC
ACCTGCCTCCTTTATATTGATCGCCATCTTGTGCATGAAGTGACGAGCCCGCAGGCCTTTGAGGGGCTGC
ACATGGCAGGCCGCCCGGTGCGCCACCCGGAAAAGACGCTTGCCGTGGTGGACCATAACGTTCCGACGTC
GCCCGACCGCATCAATGGCATCCAGAACGAGGAAAGCCGCATCCAGGTCGAGGCACTGGCCAGGAATGCC
GCCGATTTCGGCGTCGAATATTATTCCGAACGCGACAAGCGCCAGGGCATCGTGCATATCGTCGGGCCAG
AGCAGGGCTTCACCCTGCCGGGCATGACCATCGTTTGCGGTGACAGCCACACTTCCACCCACGGCGCTTT
CGGTGCGCTTGCACATGGCATCGGCACATCGGAGGTGGAGCATGTGCTCGCCACCCAGACGCTGATCCAG
AAAAAAGCCAAGAATATGCTGGTGCGCGTTGACGGCAAGTTGCCGGCGGGCGTGACCGCCAAGGATATTG
TTCTGGCGATCATTGGCGAGATCGGCACGGCAGGCGGCACCGGCTATGTCATCGAATATGCGGGCGAAGC
CATTCGCTCGCTGTCGATGGAAGGCCGCATGACGATCTGCAACATGTCGATCGAGGGCGGCGCGCGTGCC
GGTCTTATCGCTCCCGACGAGACGACGTTTGAATATATCAAGGGCCGCCCGCGTGCTCCGCAGGGCGAAA
CGCTGGAACAGGCGATCAATTACTGGAAGACGCTGCATTCGGATGAAGGGGCGCATTTCGACAAGATCGT
GACGCTCGACGCCGGCAGCCTGCCGCCCATCGTTTCCTGGGGTTCTTCGCCGGAAGACGTCGTTTCCGTC
ACGGGGGTCGTGCCCAATCCGGACGATATCGCCGACGAAACCAAGCGTGCCTCCAAGTGGCGTGCGCTCG
ACTATATGGGCCTGAAGCCCGGCACGAAAATCACGGATATCGCGGTTGATCGCGTCTTCATCGGTTCGTG
CACCAATGGCCGCATCGAAGACCTTCGCGCTGCCGCCAAGGTCGTGGAAGGCAAGAAGGTTGCCCCGACC
GTCAATGCCATGATCGTTCCGGGTTCGGGTCTCGTAAAGGAACAGGCGGAAGCCGAAGGCCTGCACAAGA
TTTTCATTGAAGCCGGTTTCGACTGGCGCGAGCCGGGCTGCTCCATGTGCCTTGCGATGAACGATGACCG
GCTGAAGCCGGGCGAGCGTTGCGCTTCGACCTCGAACCGCAATTTCGAGGGCCGTCAGGGCTTCAAGGGT
CGTACGCATCTGGTATCGCCAGCAATGGCTGCGGCGGCTGCAATTGCCGGTCACTTCGTCGATATTCGCG
CGTGGAAGTG
Protein Sequence 
>gi|17986441|ref|NP_539075.1| isopropylmalate isomerase large subunit [Brucella melitensis bv. 1 str. 16M] MSAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLHMAGRPVRHPEKTLAVVDHNVPTSPDRINGIQNEESRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGKLPAGVTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDETTFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDVVSVTGVVPNPDDIADETKRASKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRAAAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIEAGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate(UniProt: Q8FYG9).

CATALYTIC ACTIVITY: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate + H(2)O(UniProt: Q8FYG9).

CATALYTIC ACTIVITY: (2S)-2-isopropylmaleate + H(2)O = 3-hydroxy-4-methyl-3-carboxypentanoate(UniProt: Q8FYG9).

COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity)(UniProt: Q8FYG9).

PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2(UniProt: Q8FYG9).

SUBUNIT: Heterodimer of leuC and leuD (By similarity)(UniProt: Q8FYG9).

SIMILARITY: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily(UniProt: Q8FYG9).

MUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis leuC gene is required for intracellular multiplication in human macrophage THP-1 cells (Kohler et al., 2002).
COG COG0065E, under E: Amino acid transport and metabolism
References
Kohler et al., 2002: Kohler S, Foulongne V, Ouahrani-Bettache S, Bourg G, Teyssier J, Ramuz M, Liautard JP. The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. Proceedings of the National Academy of Sciences of the United States of America. 2002 Nov 26; 99(24); 15711-6. [PubMed: 12438693 ].