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dnaK from Brucella suis 1330 |
| Victor ID |
1114 |
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Gene Name |
dnaK from Brucella suis 1330 |
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Sequence Strain (Species/Organism) |
Brucella suis 1330 |
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NCBI Gene ID |
1167828
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NCBI Protein GI |
23502973
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Locus Tag |
BR2125 |
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Protein Accession |
NP_699100.1 |
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Other Database IDs |
UniProtKB-ID: DNAK_BRUSU
UniRef100: UniRef100_Q8FXX2
UniRef90: UniRef90_Q05981
UniRef50: UniRef50_Q8DH10
UniParc: UPI00000DD7BD
EMBL: AE014291
EMBL-CDS: AAN31015.1
RefSeq_NT: NC_004310.3
GenomeReviews: AE014291_GR
KEGG: bms:BR2125
NMPDR: fig|204722.1.peg.2057
TIGR: BR2125
HOGENOM: HBG334976
OMA: MKAKKDD
ProtClustDB: PRK00290
BioCyc: BSUI204722:BR_2125-MONOMER
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Taxonomy ID |
204722
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Chromosome No |
I |
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Gene Starting Position |
2049569 |
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Gene Ending Position |
2051482 |
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Gene Strand (Orientation) |
+ |
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Protein Name |
molecular chaperone DnaK |
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DNA Sequence |
>gi|56968325:2049569-2051482 Brucella suis 1330 chromosome I, complete sequence
TATGGCTAAAGTTATTGGTATCGATCTGGGAACGACCAACTCCTGCGTTGCCGTCATGGACGGAAAGAAC
GCGAAGGTCATTGAAAATGCAGAAGGTGCACGCACCACCCCTTCGATCATCGCCTTCACCGATGGTGACG
AACGCCTCGCTGGTCAGCCTGCGAAGCGTCAGGCCGTCACCAACCCGGAAGGCACCCTTTTTGCCGTCAA
GCGCCTGATTGGCCGTCGCTATGACGATCCGATGGTCACCAAGGACAAGGATCTTGTTCCTTACAAGATC
GTCAAGGGCGACAATGGCGATGCCTGGGTCGAGGTGCACGGCAAGAAATATTCTCCGTCGCAGATCTCCG
CCATGATCCTTCAGAAGATGAAGGAAACGGCTGAATCCTACCTTGGCGAAACCGTTACGCAGGCCGTCAT
CACGGTTCCGGCCTACTTCAACGACGCCCAGCGTCAGGCCACCAAGGATGCCGGCAAGATCGCCGGTCTG
GAAGTTCTGCGCATCATCAACGAGCCGACCGCGGCTGCGCTTGCCTATGGTCTCGACAAGAGCGAAGGCA
AGACCATCGCTGTCTATGACCTTGGCGGCGGTACTTTCGACGTGTCGGTTCTGGAAATCGGCGACGGCGT
TTTTGAAGTGAAGTCCACCAATGGCGACACGTTCCTTGGCGGTGAAGACTTCGATATTCGTCTGGTCGAA
TATCTGGTTGCCGAGTTCAAGAAGGAAAGTGGCATCGACCTGAAGAACGACAAGCTTGCCCTGCAGCGCC
TCAAGGAAGCTGCCGAAAAGGCCAAGATCGAACTGTCGTCCTCGCAGCAGACCGAAATCAACCTGCCGTT
CATCACGGCTGACCAGACTGGCCCGAAGCATCTGGCGATCAAGCTGTCGCGCGCCAAGTTTGAAAGCCTG
GTCGATGATCTCGTGCAGCGCACGGTCGAGCCGTGCAAGGCGGCGCTCAAGGATGCCGGCCTCAAGGCTG
GCGAAATTGACGAAGTGGTTCTGGTTGGCGGCATGACCCGCATGCCCAAGATTCAGGAAGTCGTGAAGGC
CTTCTTCGGCAAGGAACCGCACAAGGGCGTGAACCCGGATGAAGTCGTGGCCATGGGCGCGGCGATCCAG
GGCGGCGTTTTGCAGGGCGACGTCAAGGACGTGCTGCTGCTCGACGTGACCCCGCTTTCGCTCGGCATTG
AAACGCTGGGCGGCGTGTTCACCCGCCTGATCGAACGCAACACCACTATCCCGACCAAGAAGTCGCAGAC
CTTCTCCACGGCTGAGGACAACCAGTCGGCCGTGACGATCCGCGTCTTCCAGGGCGAGCGTGAAATGGCA
GCCGATAACAAGCTGCTTGGACAGTTCGACCTCGTTGGCATTCCGCCCGCACCGCGCGGTGTGCCGCAGA
TCGAAGTGACCTTCGACATCGACGCCAACGGCATCGTCAACGTTTCGGCCAAGGATAAGGGTACCGGCAA
GGAGCACCAGATCCGCATTCAGGCTTCGGGCGGCCTGACCGATGCCGACATCGAAAAGATGGTCAAGGAC
GCCGAAGCCAATGCCGAGGCCGACAAGAAGCGTCGTGAGAGCGTCGAAGCCAAGAATCAGGCCGAAAGCC
TGGTCCATTCGACCGAAAAGTCGCTGGCCGAATATGGCGACAAGGTTTCGGCCGATGACAAGAAGGCCAT
TGAGGATGCGATCGCAGCCCTCAAGACCTCGCTTGAAGGTGAAGACGCCGAGGATATCAAGGCGAAGACC
CAGGCCCTTGCCGAAGTTTCCATGAAGCTTGGTCAGGCCATGTACGAAGCAGCGCAGGCTGCCGAAGGTG
CGGGTGCCGAGGGCGGTGAACAGGCCTCTTCGTCCAAGGACGATGTGGTCGATGCCGACTACGAAGAAAT
CGACGACAACAAGAAGTCGTCGTA
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Protein Sequence |
>gi|23502973|ref|NP_699100.1| molecular chaperone DnaK [Brucella suis 1330] MAKVIGIDLGTTNSCVAVMDGKNAKVIENAEGARTTPSIIAFTDGDERLAGQPAKRQAVTNPEGTLFAVKRLIGRRYDDPMVTKDKDLVPYKIVKGDNGDAWVEVHGKKYSPSQISAMILQKMKETAESYLGETVTQAVITVPAYFNDAQRQATKDAGKIAGLEVLRIINEPTAAALAYGLDKSEGKTIAVYDLGGGTFDVSVLEIGDGVFEVKSTNGDTFLGGEDFDIRLVEYLVAEFKKESGIDLKNDKLALQRLKEAAEKAKIELSSSQQTEINLPFITADQTGPKHLAIKLSRAKFESLVDDLVQRTVEPCKAALKDAGLKAGEIDEVVLVGGMTRMPKIQEVVKAFFGKEPHKGVNPDEVVAMGAAIQGGVLQGDVKDVLLLDVTPLSLGIETLGGVFTRLIERNTTIPTKKSQTFSTAEDNQSAVTIRVFQGEREMAADNKLLGQFDLVGIPPAPRGVPQIEVTFDIDANGIVNVSAKDKGTGKEHQIRIQASGGLTDADIEKMVKDAEANAEADKKRRESVEAKNQAESLVHSTEKSLAEYGDKVSADDKKAIEDAIAALKTSLEGEDAEDIKAKTQALAEVSMKLGQAMYEAAQAAEGAGAEGGEQASSSKDDVVDADYEEIDDNKKSS
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Molecule Role |
Virulence factor |
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Molecule Role Annotation |
FUNCTION: Acts as a chaperone (By similarity)(UniProt: Q8FXX2).
INDUCTION: By stress conditions e.g. heat shock (By similarity)(UniProt: Q8FXX2).
SIMILARITY: Belongs to the heat shock protein 70 family(UniProt: Q8FXX2).
MUTATION: The heat shock protein DnaK is essential for intramacrophagic replication of Brucella suis. The replacement of the stress-inducible, native dnaK promoter of B suis by the promoter of the constitutively expressed bla gene resulted in temperature-independent synthesis of DnaK. In contrast to a dnaK null mutant, this strain grew at 37 degrees C, with a thermal cutoff at 39 degrees C However, the constitutive dnaK mutant, which showed high sensitivity to H(2)O(2)-mediated stress , failed to multiply in murine macrophage-like cells and was rapidly eliminated in a mouse model of infection, adding strong arguments that stress-mediated and heat shock promoter-dependent induction of dnaK is a crucial event in the intracellular replication of B suis (Köhler et al., 2002).
Mutation studies indicated that DnaK, but not DnaJ, was required for growth at 37 degrees C in vitro. Viability of the dnaK null mutant was also greatly affected at low pH. In infection experiments performed with both mutants at the reduced temperature of 30 degrees C, the dnaK mutant of B suis survived but failed to multiply within U937 cells, whereas the wild-type strain and the dnaJ mutant multiplied normally. Complementation of the dnaK mutant with the cloned dnaK gene restored growth at 37 degrees C, increased resistance to acid pH, and increased intracellular multiplication (Köhler et al., 2002). |
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COG
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COG0443O, under O: Posttranslational modification, protein turnover, chaperones |
| References |
Köhler et al., 2002: Köhler S, Ekaza E, Paquet JY, Walravens K, Teyssier J, Godfroid J, Liautard JP. Induction of dnaK through its native heat shock promoter is necessary for intramacrophagic replication of Brucella suis. Infection and immunity. 2002; 70(3); 1631-1634. [PubMed: 11854256].
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