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leuC from Brucella suis 1330

Victor ID 1104
Gene Name leuC from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1167606
NCBI Protein GI 23502757
Locus Tag BR1906
Protein Accession NP_698884.1
Other Database IDs UniProtKB-ID: LEUC_BRUSU
UniRef100: UniRef100_B2S860
UniRef90: UniRef90_B2S860
UniRef50: UniRef50_C1F700
UniParc: UPI00000DD71D
EMBL: AE014291
EMBL-CDS: AAN30799.1
RefSeq_NT: NC_004310.3
GenomeReviews: AE014291_GR
KEGG: bms:BR1906
TIGR: BR1906
HOGENOM: HBG330745
OMA: RPHAPKG
ProtClustDB: PRK05478
BioCyc: BSUI204722:BR_1906-MONOMER
Taxonomy ID 204722
Chromosome No I
Gene Starting Position 1840511
Gene Ending Position 1841920
Gene Strand (Orientation) -
Protein Name isopropylmalate isomerase large subunit
DNA Sequence 
>gi|56968325:1840511-1841920 Brucella suis 1330 chromosome I, complete sequence
CTCACTTCCACGCGCGAATATCGACGAAGTGACCGGCAATTGCAGCCGCCGCAGCCATTGCTGGCGATAC
CAGATGCGTACGACCCTTGAAGCCCTGACGGCCCTCGAAATTGCGGTTCGAGGTCGAAGCGCAACGCTCG
CCCGGCTTCAGCCGGTCATCGTTCATCGCAAGGCACATGGAGCAGCCCGGCTCGCGCCAGTCGAAACCGG
CTTCAATGAAAATCTTGTGCAGGCCTTCGGCTTCCGCCTGTTCCTTTACGAGACCCGAACCCGGAACGAT
CATGGCATTGACGGTCGGGGCAACCTTCTTGCCTTCCACGACCTTGGCGGCAGCGCGAAGGTCTTCGATG
CGGCCATTGGTGCACGAACCGATGAAGACGCGATCAACCGCGATATCCGTGATTTTCGTGCCGGGCTTCA
GGCCCATATAGTCGAGCGCACGCCACTTGGAGGCACGCTTGGTTTCGTCGGCGATATCGTCCGGATTGGG
CACGACCCCCGTGACGGAAACGACGTCTTCCGGCGAAGAACCCCAGGAAACGATGGGCGGCAGGCTGCCG
GCGTCGAGCGTCACGATCTTGTCGAAATGCGCCCCTTCATCCGAATGCAGCGTCTTCCAGTAATTGATCG
CCTGTTCCAGCGTTTCGCCCTGCGGAGCACGCGGGCGGCCCTTGATATATTCAAACGTCGTCTCGTCGGG
AGCGATAAGACCGGCACGCGCGCCGCCCTCGATCGACATGTTGCAGATCGTCATGCGGCCTTCCATCGAC
AGCGAGCGAATGGCTTCGCCCGCATATTCGATGACATAGCCGGTGCCGCCTGCCGTGCCGATCTCGCCAA
TGATCGCCAGAACAATATCCTTGGCGGTCACGCCCGCCGGCAATTTGCCGTCAACGCGCACCAGCATATT
CTTGGCTTTTTTCTGGATCAGCGTCTGGGTGGCGAGCACATGCTCCACCTCCGATGTGCCGATGCCATGT
GCAAGCGCACCGAAAGCGCCGTGGGTGGAAGTGTGGCTGTCACCGCAAACGATGGTCATGCCCGGCAGGG
TGAAGCCCTGCTCTGGCCCGACGATATGCACGATGCCCTGGCGCTTGTCGCGTTCGGAATAATATTCGAC
GCCGAAATCGGCGGCATTCCTGGCCAGTGCCTCGACCTGGATGCGGCTTTCCTCGTTCTGGATGCCATTG
ATGCGGTCGGGCGACGTCGGAACGTTATGGTCCACCACGGCAAGCGTCTTTTCCGGGTGGCGCACCGGGC
GGCCTGCCATGCGCAGCCCCTCAAAGGCCTGCGGGCTCGTCACTTCATGCACAAGATGGCGATCAATATA
AAGGAGGCAGGTGCCGTCTTCCTGCTGATCCACTACATGGTCATCCCAAATCTTGTCATAAAGTGTACGC
GGCGCGCTCA
Protein Sequence 
>gi|23502757|ref|NP_698884.1| isopropylmalate isomerase large subunit [Brucella suis 1330] MSAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRPVRHPEKTLAVVDHNVPTSPDRINGIQNEESRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGKLPAGVTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDETTFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDVVSVTGVVPNPDDIADETKRASKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRAAAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIEAGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate(UniProt: Q8FYG9).

CATALYTIC ACTIVITY: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate + H(2)O(UniProt: Q8FYG9).

CATALYTIC ACTIVITY: (2S)-2-isopropylmaleate + H(2)O = 3-hydroxy-4-methyl-3-carboxypentanoate(UniProt: Q8FYG9).

COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity)(UniProt: Q8FYG9).

PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2(UniProt: Q8FYG9).

SUBUNIT: Heterodimer of leuC and leuD (By similarity)(UniProt: Q8FYG9).

SIMILARITY: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily(UniProt: Q8FYG9).

MUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis leuC gene is required for intracellular multiplication in human macrophage THP-1 cells (Kohler et al., 2002).
COG COG0065E, under E: Amino acid transport and metabolism
References
Kohler et al., 2002: Kohler S, Foulongne V, Ouahrani-Bettache S, Bourg G, Teyssier J, Ramuz M, Liautard JP. The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. Proceedings of the National Academy of Sciences of the United States of America. 2002 Nov 26; 99(24); 15711-6. [PubMed: 12438693 ].