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purE from Brucella suis 1330

Victor ID 1100
Gene Name purE from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1167437
NCBI Protein GI 23502601
Locus Tag BR1744
Protein Accession NP_698728.1
Other Database IDs UniProtKB-ID: PURE_BRUSU
UniRef100: UniRef100_Q8FYW3
UniRef90: UniRef90_P52558
UniRef50: UniRef50_Q5E1R4
UniParc: UPI00000DD6A0
EMBL: AE014291
EMBL-CDS: AAN30643.1
RefSeq_NT: NC_004310.3
GenomeReviews: AE014291_GR
KEGG: bms:BR1744
NMPDR: fig|204722.1.peg.1685
TIGR: BR1744
HOGENOM: HBG301965
OMA: GVVMGSS
ProtClustDB: CLSK898071
BioCyc: BSUI204722:BR_1744-MONOMER
Taxonomy ID 204722
Chromosome No I
Gene Starting Position 1679425
Gene Ending Position 1679913
Gene Strand (Orientation) +
Protein Name phosphoribosylaminoimidazole carboxylase, catalytic subunit
DNA Sequence 
>gi|56968325:1679425-1679913 Brucella suis 1330 chromosome I, complete sequence
GATGAGCGTTGATGTCGCCATTATCATGGGAAGCCAGTCCGATTGGGAAACCATGCGCCATGCAGCCCAC
ACATTGGAGGCGCTCGGCATCTCCTTCGACGCACGGATCGTTTCCGCCCATCGCACCCCTGACAGGCTGG
TCGCCTTCGCCAAGGGGGCGAAAGCGGAAGGCTTCAAGGTCATCATCGCAGGCGCCGGCGGCGCGGCCCA
CCTGCCCGGCATGGCCGCTGCCATGACACCGCTTCCCGTCTTTGGCGTTCCAGTTCAATCCAAGGCGCTT
TCGGGCCAGGATTCGCTACTCTCCATCGTACAGATGCCAGCGGGTATTCCCGTCGGCACTCTCGCCATCG
GCCGTGCAGGCGCGGTCAATGCCGCCCTTCTCGCCGCCGCCGTTCTGGCGCTATATGATGAAGCACTTGC
CGCCCGTCTTGATGAATGGCGCAAGGCGCAGACCGAAAGCGTTGCAGAACGCCCCTCGAACGAAGCCTG
Protein Sequence 
>gi|23502601|ref|NP_698728.1| phosphoribosylaminoimidazole carboxylase, catalytic subunit [Brucella suis 1330] MSVDVAIIMGSQSDWETMRHAAHTLEALGISFDARIVSAHRTPDRLVAFAKGAKAEGFKVIIAGAGGAAHLPGMAAAMTPLPVFGVPVQSKALSGQDSLLSIVQMPAGIPVGTLAIGRAGAVNAALLAAAVLALYDEALAARLDEWRKAQTESVAERPSNEA
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: This subunit can alone transform AIR to CAIR, but in association with purK, which possesses an ATPase activity, an enzyme complex is produced which is capable of converting AIR to CAIR efficiently under physiological condition (By similarity)(UniProt: Q8FYW3).

CATALYTIC ACTIVITY: 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2)(UniProt: Q8FYW3).

PATHWAY: Nucleotide biosynthesis; IMP biosynthesis; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 3(UniProt: Q8FYW3).

PATHWAY: Context: Purine biosynthesis(UniProt: Q8FYW3).

SUBUNIT: Homooctamer (By similarity)(UniProt: Q8FYW3).

SIMILARITY: Belongs to the AIR carboxylase family(UniProt: Q8FYW3).

MUTATION: Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALBc mouse model. It confirms the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages (Alcantara et al., 2004). The purE mutant has reduced survival in murine macrophages and reduced virulence in mice (Alcantara et al., 2004).

A purE gene deletion mutant (purE201) of B melitensis was constructed as a potentially useful vaccine for humans and animals. At necropsy, bacteria were present in mammary lymph nodes or spleen of 33 of goats given virulent 16M but in none of goats given the purE mutant. The purE mutation of B melitensis 16M was stable and that the vaccine could be differentiated from wild-type strains by hybridization, purine auxotrophy, and kanamycin resistance. Mice clear the identical purE mutant strain from their spleens in only 8 weeks but remain infected with B. melitensis 16M for at least 3 months (Alcantara et al., 2004).
COG COG0041F, under F: Nucleotide transport and metabolism
References
Alcantara et al., 2004: Alcantara RB, Read RD, Valderas MW, Brown TD, Roop RM 2nd. Intact purine biosynthesis pathways are required for wild-type virulence of Brucella abortus 2308 in the BALB/c mouse model. Infection and immunity. 2004; 72(8); 4911-4917. [PubMed: 15271960].