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leuA from Brucella suis 1330

Victor ID 1094
Gene Name leuA from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1167249
NCBI Protein GI 161486694
Locus Tag BR1566
Protein Accession NP_698557.2
Other Database IDs UniProtKB-ID: LEU1_BRUSU
UniRef100: UniRef100_Q8FZC4
UniRef90: UniRef90_Q8FZC4
UniRef50: UniRef50_A4QAP0
UniParc: UPI000021CACD
EMBL: AE014291
EMBL-CDS: AAN30472.1
RefSeq_NT: NC_004310.3
GenomeReviews: AE014291_GR
KEGG: bms:BR1566
NMPDR: fig|204722.1.peg.1514
TIGR: BR1566
HOGENOM: HBG289752
ProtClustDB: PRK03739
BioCyc: BSUI204722:BR_1566-MONOMER
Taxonomy ID 204722
Chromosome No I
Gene Starting Position 1515904
Gene Ending Position 1517571
Gene Strand (Orientation) -
Protein Name 2-isopropylmalate synthase
DNA Sequence 
>gi|56968325:1515904-1517571 Brucella suis 1330 chromosome I, complete sequence
TCTACTTCGCAATCACACGATTTGCCGCCGACACCACGGCTTCAAGCGAGGCGCTGACGATATTATCGTT
GATGCCGACACCGAACAGCTTGCCGCCGGGATAAACCATCTCGACATAGGAAATGGCCGAAGCATCCGAA
CCCTGCTGGAGCGAATGTTCGGAATAATCGACCACCGACATTTTCACGCCAAGATATTTCGACAGCGCAT
CCACGAAACCATCGATCGGTCCCGTGCCCTTGCCCTCGATAGTCTTGGTGACACCGTTATCGGTGATCTC
AGCCGTCAGGATGCGCCGGCCCTTTTGCTCAGGATCGGGATAGGTGTGGTGATCCACGAACTTGATGCGG
GCATCGGGCTGCGTCACATAGAGTTTCTGGAACTCCTCGTAAATGCGCTTCGATGGCAGCTCCTTGCCCT
CTTCATCCGTAATGTGCTGGATGATTTCGCGGAACTCGACCTGCAGGTTGCGCGGCAGGTTCAGGCCGTA
ATCGGCTTGCAGGATATAGGCGATACCGCCCTTGCCCGATTGCGAATTGATGCGGATGATCGCCTCGTAG
GAGCGGCCCACATCCTGCGGATCGATCGGCAGGTAAGGAACTTCCCAAACCGGCGAATTGGCCGAACGGC
GCGCTTTCATGCCCTTGTTGATCGCATCCTGATGCGAGCCGGAGAAAGCCGTATAGACCAGCTCGCCCAC
ATAAGGATGGCGCTCGGCGATCTTCAACTGGTTGGAATATTCGTAGACTTCCTTCATCCGGTTGATATCG
GTGCAATCCAGCCCGGGGTCGATGCCCTGCGTGTACATGTTGAGCGCCAGCGTCACCACATCCACATTGC
CCGTGCGCTCGCCATTGCCGAACAGCGTGCCTTCCACGCGATCAGCGCCTGCCATCAGGCCCAGTTCAGT
GGCGGCAATGCCCGTGCCGCGGTCATTATGCGGATGCAGCGAGATAATCAGGCTCTCGCGATTGTCCAGA
TTGCGGCACATCCATTCGATCTGGTCGGCATAGACATTCGGCGTGTTCATCTCCACCGTCGAAGGCAGGT
TGACGATCAGCTTGTTGTCCGGCGTCGGCTTCACGATCTCGATCACCGCATTGCAGATTTCCAGCGCCAC
ATCCAGTTCGGTGCCGGTAAAGCTTTCCGGCGAATACTGGAAGCGATAACCGCCGCCAGCCTTGGCAGCC
ATATCCATGATCATCTTGGCCGCATCGGTTGCAATCTGCTTGATGCCGCCGACATCCTTGGCAAACACCA
CGCGGCGCTGCAATTCGCTGGTGGAATTGTAGAAATGGATGATCGGCGTCTTCGCGCCTTCCAGCGCCTC
GAAAGTGCGCGTAATCAATTCAGGACGGCACTGGACCAGAACCTGCAAATCCACGTCGTCAGGAACATCG
CCCTGTTCAATGGCCCAGCGGCAGAAGTCGAAATCCGTCTGCGAAGCGGACGGGAAACCGATCTCGATTT
CCGGGAAACCCATATCGATCAGCAGGCGGAACATGCGCTCCTTGCGGTCATGCCCCATGGGGTCGATCAA
AGCCTGGTTGCCGTCGCGCAGATCGACCGAACACCAGATCGGCGCCTTTTCGATGCGTTTGGAAGGCCAA
GTGCGGTCATCGAGCTGCGGCGCAGGGAACGGCGCGTATTTGGTACCGGCAATAGGCA
Protein Sequence 
>gi|161486694|ref|NP_698557.2| 2-isopropylmalate synthase [Brucella suis 1330] MPIAGTKYAPFPAPQLDDRTWPSKRIEKAPIWCSVDLRDGNQALIDPMGHDRKERMFRLLIDMGFPEIEIGFPSASQTDFDFCRWAIEQGDVPDDVDLQVLVQCRPELITRTFEALEGAKTPIIHFYNSTSELQRRVVFAKDVGGIKQIATDAAKMIMDMAAKAGGGYRFQYSPESFTGTELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVYADQIEWMCRNLDNRESLIISLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGIDPGLDCTDINRMKEVYEYSNQLKIAERHPYVGELVYTAFSGSHQDAINKGMKARRSANSPVWEVPYLPIDPQDVGRSYEAIIRINSQSGKGGIAYILQADYGLNLPRNLQVEFREIIQHITDEEGKELPSKRIYEEFQKLYVTQPDARIKFVDHHTYPDPEQKGRRILTAEITDNGVTKTIEGKGTGPIDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVEMVYPGGKLFGVGINDNIVSASLEAVVSAANRVIAK
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)(UniProt: Q8FZC4).

CATALYTIC ACTIVITY: Acetyl-CoA + 3-methyl-2-oxobutanoate + H(2)O = (2S)-2-isopropylmalate + CoA(UniProt: Q8FZC4).

PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1(UniProt: Q8FZC4).

SUBUNIT: Homotetramer (By similarity)(UniProt: Q8FZC4).

SIMILARITY: Belongs to the alpha-IPM synthetase/homocitrate synthase family. LeuA type 2 subfamily(UniProt: Q8FZC4).

MUTATION: leuA is a B. suis gene identified by signature-tagged mutagenesis. It is attenuated inside THP1 macrophage cell line.
COG COG0119E, under E: Amino acid transport and metabolism
References