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pncA from Brucella suis 1330

Victor ID 1090
Gene Name pncA from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1167147
NCBI Protein GI 23502334
Locus Tag BR1465
Protein Accession NP_698461.1
Other Database IDs UniProtKB-ID: Q8FZL5_BRUSU
UniRef100: UniRef100_E0DY99
UniRef90: UniRef90_E0DY99
UniRef50: UniRef50_C3KLM5
UniParc: UPI00000DD5D1
EMBL: AE014291
EMBL-CDS: AAN30376.1
RefSeq_NT: NC_004310.3
GenomeReviews: AE014291_GR
KEGG: bms:BR1465
NMPDR: fig|204722.1.peg.1418
TIGR: BR1465
HOGENOM: HBG289389
OMA: LYLEVID
ProtClustDB: PRK14013
BioCyc: BSUI204722:BR_1465-MONOMER
Taxonomy ID 204722
Chromosome No I
Gene Starting Position 1422178
Gene Ending Position 1423239
Gene Strand (Orientation) -
Protein Name hypothetical protein
DNA Sequence 
>gi|56968325:1422178-1423239 Brucella suis 1330 chromosome I, complete sequence
TCTAGGTCACTTCCTCGGTCTCGTTCTGCCAGTTCGGGTTCAGGCGGTTATAGCGCAGCGACGACATGAA
GGAGAGGCCGATAAGTGCCGCGCCGATGAGGCCGGTAATAACTTCCGGTATATGCACCAGCGTCTGTACA
TACATGATGATCGCCAGCACGAGGATAGCGTAAAACGCACCGTGCTCCAGATACCGGTACTGGCCAAGCG
TCTTGCGCTCCACCAGCATGATCGTGAGCGAGCGCACATAAAACGCGCCGATACCAAGGCCGATGGCGAT
CACGAAAAGGTTCGTGGTGAGTGCGAAAGCACCGATCACGCCGTCAAAGGAAAAGCTGGCATCCAGCACT
TCCAGATAGATAAACGCACCAAGACCGCCGCGATGGACCATATCCATCTGCTCCTGCGTCGCATCCAGAA
CTTCGCCAAGCCCTTCCACTGCAAGGAAGGTCAGAAGACCAAACAGCCCCGCAATAACGAATGTATGCGA
ATGGTCGGCGTCAAGCTGGTAGGAGAAGAAGATGATAAGGGCAATGGCGACGCCGATTTCAACACCCTGA
ACGGATGCGAATTTCGACATGCGGCTCTCGATCATCCTGATCCAGTGCACATCCTTTTCAACGTCGAAGA
AATATTTCATGCCGACCATCAGAAGGAACGTGCCGCCGAACGCGGCGATGCCCGTATGGGATTCCGCAAT
CACGCGCGCATATTCATTGGGCTGCCAGATGGCAAGTTTCAGCGCCGAGATCGGGTCCGTCCACATGGCT
ATGGCCACGATTGCCAGCGGGAAGACGATGCGCATGCCGAAAACGGCGATGACGATGCCCCATGTCAGGA
AGCGGTGCTGCCATTTCGGTGACATATCGCGCAGGATACGCGCATTGACGATGGCATTATCGAAGGAAAG
CGAGATTTCGAGAACGCTCAAAACCGCACAAATGATGAAAACGGAAACCATTCCCCCAAAAGTGCCGGTC
AGTTCGTAACCGAGCCATACGGCACCGGCCAAACCCAAAACGGTGAAAATCATGGGCCAAATAAAATAAC
GATAGGCGGCCA
Protein Sequence 
>gi|23502334|ref|NP_698461.1| hypothetical protein BR1465 [Brucella suis 1330] MAAYRYFIWPMIFTVLGLAGAVWLGYELTGTFGGMVSVFIICAVLSVLEISLSFDNAIVNARILRDMSPKWQHRFLTWGIVIAVFGMRIVFPLAIVAIAMWTDPISALKLAIWQPNEYARVIAESHTGIAAFGGTFLLMVGMKYFFDVEKDVHWIRMIESRMSKFASVQGVEIGVAIALIIFFSYQLDADHSHTFVIAGLFGLLTFLAVEGLGEVLDATQEQMDMVHRGGLGAFIYLEVLDASFSFDGVIGAFALTTNLFVIAIGLGIGAFYVRSLTIMLVERKTLGQYRYLEHGAFYAILVLAIIMYVQTLVHIPEVITGLIGAALIGLSFMSSLRYNRLNPNWQNETEEVT
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: Brucella abortus pnc mutant via mini-Tn5Km2 transposon mutagenesis has intracellular growth defect inside HeLa cells (Kim et al., 2003). Nicotinamidasepyrazinamidase mutant (pncA mutant) of Brucella abortus failed to replicate in HeLa cells, and showed a lower rate of intracellular replication than that of wild-type strain in macrophages (Kim et al., 2004). The pncA mutant was not co-localizing with either late endosomes or lysosomes. The pncA mutant showed a 1.5-log reduction of the number of bacteria isolated from mouse spleens after 10 weeks (Kim et al., 2004). It indicates that the mutant has reduced virulence in mice.
COG COG2899S, under S: Function unknown
References
Kim et al., 2003: Kim S, Watarai M, Kondo Y, Erdenebaatar J, Makino S, Shirahata T. Isolation and characterization of mini-Tn5Km2 insertion mutants of Brucella abortus deficient in internalization and intracellular growth in HeLa cells. Infection and immunity. 2003; 71(6); 3020-3027. [PubMed: 12761078].
Kim et al., 2004: Kim S, Kurokawa D, Watanabe K, Makino S, Shirahata T, Watarai M. Brucella abortus nicotinamidase (PncA) contributes to its intracellular replication and infectivity in mice. FEMS microbiology letters. 2004; 234(2); 289-295. [PubMed: 15135535].