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pncA from Brucella suis 1330

Victor ID 1089
Gene Name pncA from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1167146
NCBI Protein GI 23502333
Locus Tag BR1464
Protein Accession NP_698460.1
Other Database IDs UniProtKB-ID: Q8FZL6_BRUSU
UniRef100: UniRef100_Q8FZL6
UniRef90: UniRef90_B2S6T3
UniRef50: UniRef50_Q87ZX7
UniParc: UPI00000DD5D0
EMBL: AE014291
EMBL-CDS: AAN30375.1
RefSeq_NT: NC_004310.3
HSSP: O58727
GenomeReviews: AE014291_GR
KEGG: bms:BR1464
NMPDR: fig|204722.1.peg.1417
TIGR: BR1464
HOGENOM: HBG752434
OMA: FRKGTDP
ProtClustDB: CLSK898153
BioCyc: BSUI204722:BR_1464-MONOMER
Taxonomy ID 204722
Chromosome No I
Gene Starting Position 1421552
Gene Ending Position 1422181
Gene Strand (Orientation) +
Protein Name pyrazinamidase/nicotinamidase
DNA Sequence 
>gi|56968325:1421552-1422181 Brucella suis 1330 chromosome I, complete sequence
CATGATCGGCCATGCACTTGTGGTTATCGACGTCCAGAATGATTTTTGCCCCGGCGGCGCCTTGGCCATC
GAACGTGGCGATGAAATCATCCCGATCGTCAACCGCCTGATCGGGGAAAGCGAAAATGTCGTGGTCACGC
AGGATTGGCATCCGGCCAACCATTCCAGCTTCGCCTCCAACCACCCCGGCAGCCGCCCGTTCGATACGGT
CAATATGTCTTATGGGCCGCAGGTGCTCTGGCCGGATCATTGCGTGCAAAATAGCGAGGGGGCAGAATTT
CACCCCGATCTGCAATGGTGGCGTGCACAGCTTGTCGTCCGCAAGGGTGTCCACATTGGTATCGACAGCT
ACTCCGCATTTTTTGAAAATGACCATTGCACGCCGACGGGCCTTGGCGGATATTTGCGTGAACGCAATAT
CGGATCATTGACAATGGTTGGCCTCGCCACCGATTTCTGCGTCGCCAGTTCAGCGCTTGATGCGGTCCAG
CAGGGCTTTCAGGTGCGGGTGCGACTCGATGCCTGCCGCGGTATTGATCTCAACGGGTCGATGAACATCA
TGCTGGAGAAAATGAAACAGGCTGGCGTCGAACTGATCGATGAGCCTGTTTCAGCGTCGCATCTACTCTA
Protein Sequence 
>gi|23502333|ref|NP_698460.1| pyrazinamidase/nicotinamidase [Brucella suis 1330] MIGHALVVIDVQNDFCPGGALAIERGDEIIPIVNRLIGESENVVVTQDWHPANHSSFASNHPGSRPFDTVNMSYGPQVLWPDHCVQNSEGAEFHPDLQWWRAQLVVRKGVHIGIDSYSAFFENDHCTPTGLGGYLRERNIGSLTMVGLATDFCVASSALDAVQQGFQVRVRLDACRGIDLNGSMNIMLEKMKQAGVELIDEPVSASHLL
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: Brucella abortus pnc mutant via mini-Tn5Km2 transposon mutagenesis has intracellular growth defect inside HeLa cells (Kim et al., 2003). Nicotinamidasepyrazinamidase mutant (pncA mutant) of Brucella abortus failed to replicate in HeLa cells, and showed a lower rate of intracellular replication than that of wild-type strain in macrophages (Kim et al., 2003). The pncA mutant was not co-localizing with either late endosomes or lysosomes. The pncA mutant showed a 1.5-log reduction of the number of bacteria isolated from mouse spleens after 10 weeks (Kim et al., 2003). It indicates that the mutant has reduced virulence in mice.
COG COG1335Q, under Q: Secondary metabolites biosynthesis, transport and catabolism
References
Kim et al., 2003: Kim S, Watarai M, Kondo Y, Erdenebaatar J, Makino S, Shirahata T. Isolation and characterization of mini-Tn5Km2 insertion mutants of Brucella abortus deficient in internalization and intracellular growth in HeLa cells. Infection and immunity. 2003; 71(6); 3020-3027. [PubMed: 12761078].