Search: for Help
About
Introduction
Statistics
Your PHIDIAS
Register or Login
Philert
Submission
Curated Data
Victors
BBP (Brucella)
Phinfo
Phinet
HazARD
Data Analysis
Phigen
Pacodom
BLAST
Help & Documents
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

miaA from Brucella suis 1330

Victor ID 1087
Gene Name miaA from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1167072
NCBI Protein GI 23502261
Locus Tag BR1390
Protein Accession NP_698388.1
Other Database IDs UniProtKB-ID: MIAA_BRUSU
UniRef100: UniRef100_Q8CY40
UniRef90: UniRef90_B2S6L5
UniRef50: UniRef50_A9M648
UniParc: UPI00000E4798
EMBL: AE014291
EMBL-CDS: AAN30303.1
RefSeq_NT: NC_004310.3
GenomeReviews: AE014291_GR
KEGG: bms:BR1390
NMPDR: fig|204722.1.peg.1345
TIGR: BR1390
HOGENOM: HBG559459
OMA: VNADSMQ
ProtClustDB: PRK00091
BioCyc: BSUI204722:BR_1390-MONOMER
Taxonomy ID 204722
Chromosome No I
Gene Starting Position 1345092
Gene Ending Position 1346024
Gene Strand (Orientation) -
Protein Name tRNA delta(2)-isopentenylpyrophosphate transferase
DNA Sequence 
>gi|56968325:1345092-1346024 Brucella suis 1330 chromosome I, complete sequence
TTTATTGCGGATCCCGAAACGAACCGCCCTGAAACACCTCTTCGCCACTGGCATAGACACGCCAGTCTTC
GCCCAACTGATTGCGAAACCAGGTGGACTGGCGCTTGGCATATTGCCGCGTGGCAATGACGGAACGTTCA
ATAGCCTCCTCGCGCGACATGGTTTCTGCAAGAAATGCACTGACTTCCCTGACGCCAATGGCCTTCATGG
CGGGTAGCGCAGGATCGAGATCGAGCGCCAGAAGCGCCCTCACCTCATCGATTGCCCCCTCCGCCCACAT
GGCCGAAAAACGCCGCGCAATACGCTCGCCAAGCCATGCACGATCAGGCCGGAGCACGATCTTTTGCGCC
GATTGGTCATCCACCAGCGCCCTGCCCTTGACCTTTTGCCATTCCAAAAGGGATTTGCCGGTGCTTTCCA
GCACTTCCAGCGCACGCACAATGCGCTGGCTATCGGAGGGCTGCAAGGCAGCCGCGATGGCCGGGTCACG
CACACAAAGGACAGCGTGCAGGGCCTTTGCGCCTTCGGCTTCCATCCGGCCTCGCCAATGGTCGCGCACT
TGCGCCGATACTTCCGGCATTTGCGAAAGCCCGCCCAGAAGCGCACGAAAATAAAGCCCTGTCCCGCCGA
CGAAGATCGGCACCCGGCCTTGCAGTCCGCAACGCCCCAGAAGTGCTTCGACATCCTCAAACCATTTGCC
GGTGGAATAGGTGCTGGAGGGCGGCACATGGCCATAAAGAAAATGTTCGGCCTCGGCCAGATCGGCCCTG
GAAGGCCGCGCGGTCAAAAGATCCAGAACGCCATAAACCTGCATGGAATCGGTATTGACGATGAAACCGC
CCGTCGCTTTGGCCATGCGAATGGCAAGTGCCGACTTGCCGCTGGCCGTTGGGCCAGCTATCAGGATCGC
ATTCTTGACTGCATCCTCACTCA
Protein Sequence 
>gi|23502261|ref|NP_698388.1| tRNA delta(2)-isopentenylpyrophosphate transferase [Brucella suis 1330] MSEDAVKNAILIAGPTASGKSALAIRMAKATGGFIVNTDSMQVYGVLDLLTARPSRADLAEAEHFLYGHVPPSSTYSTGKWFEDVEALLGRCGLQGRVPIFVGGTGLYFRALLGGLSQMPEVSAQVRDHWRGRMEAEGAKALHAVLCVRDPAIAAALQPSDSQRIVRALEVLESTGKSLLEWQKVKGRALVDDQSAQKIVLRPDRAWLGERIARRFSAMWAEGAIDEVRALLALDLDPALPAMKAIGVREVSAFLAETMSREEAIERSVIATRQYAKRQSTWFRNQLGEDWRVYASGEEVFQGGSFRDPQ
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Catalyzes the first step in the biosynthesis of 2-methylthio-N6-(delta(2)-isopentenyl)-adenosine (MS[2]I[6]A) adjacent to the anticodon of several tRNA species (By similarity)(UniProt: Q8CY40).

CATALYTIC ACTIVITY: Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine(UniProt: Q8CY40).

SIMILARITY: Belongs to the IPP transferase family(UniProt: Q8CY40).

MUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis miaA gene is required for intracellular multiplication in human macrophage THP-1 cells (Kohler et al., 2002).
COG COG0324J, under J: Translation, ribosomal structure and biogenesis
References
Kohler et al., 2002: Kohler S, Foulongne V, Ouahrani-Bettache S, Bourg G, Teyssier J, Ramuz M, Liautard JP. The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. Proceedings of the National Academy of Sciences of the United States of America. 2002 Nov 26; 99(24); 15711-6. [PubMed: 12438693 ].