Search: for Help
About
Introduction
Statistics
Your PHIDIAS
Register or Login
Philert
Submission
Curated Data
Victors
BBP (Brucella)
Phinfo
Phinet
HazARD
Data Analysis
Phigen
Pacodom
BLAST
Help & Documents
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

rpoA from Brucella suis 1330

Victor ID 1082
Gene Name rpoA from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1166885
NCBI Protein GI 23502086
Locus Tag BR1209
Protein Accession NP_698213.1
Other Database IDs UniProtKB-ID: RPOA_BRUSU
UniRef100: UniRef100_B0CH07
UniRef90: UniRef90_B2S655
UniRef50: UniRef50_Q5HAU5
UniParc: UPI00000DD51B
EMBL: AE014291
EMBL-CDS: AAN30128.1
RefSeq_NT: NC_004310.3
GenomeReviews: AE014291_GR
KEGG: bms:BR1209
NMPDR: fig|204722.1.peg.1170
TIGR: BR1209
HOGENOM: HBG430844
OMA: FGTTLGN
ProtClustDB: PRK05182
BioCyc: BSUI204722:BR_1209-MONOMER
Taxonomy ID 204722
Chromosome No I
Gene Starting Position 1186790
Gene Ending Position 1187803
Gene Strand (Orientation) -
Protein Name DNA-directed RNA polymerase subunit alpha
DNA Sequence 
>gi|56968325:1186790-1187803 Brucella suis 1330 chromosome I, complete sequence
CTCAATATTGGTCTTCGTAGCGCTTGGCGAGGTCTTCGATATTTTCCGGCGGCCAGGCTGGGATTTCCAT
ACCGAGATGGAGACCCATCGATGCCAGAACTTCCTTGATCTCGTTAAGCGACTTGCGGCCGAAATTCGGG
GTCCGCAGCATTTCGGCTTCCGTCTTCTGGATCAGATCGCCGATATAGACGATGTTGTCGGTCTTCAGGC
AGTTTGCCGAACGCACCGACAGCTCCAGTTCGTCCACCTTCTTGAGCAGAGCCGGATTGAAGGCCAGTTC
CGCAACCTGTTCCTGCGGAGCTTCCTTCTGCGGCTCTTCAAAGTTGACGAAGATCGAAAGCTGGTCCTGG
AGAATGCGAGCGGCATAAGCCACAGCGTCTTCACCCGTAACCGAACCATTGGTCTCGATATTCAGCGTCA
GCTTGTCATAGTCGAGAACCTGGCCTTCACGGGTGTTCTCGATCTTGTACGACACCTTGCGAACCGGAGA
ATAAAGGCTATCGACCGGGATCAGACCGATCGGAGCATCTTCCGCGCGGTTGCGGTCGGCAGGCACATAT
CCCTTGCCGGTGTTGACGGTGAATTCCATGCGGATTTCAGCGCCTTCATCCAGCGTGCAGATGACGTGTT
CCGGGTTCAGGATCTCGACATCGCCAACGGTCTGAATGTCGCCAGCCGTAACGACGCCGGGGCCTTCCTT
GCGGACGACCATGCGCTTCGGGCCCTCGCCTTCCATACGGATGGCGATTTCCTTGATGTTCAGAACGATA
TCCGTGACGTCTTCGCGAACGCCCGGAATGGAAGAGAATTCATGCAGAACGCCGTCGATCTGGACAGCGG
TCACGGCAGCGCCGCGAAGCGACGACAGAAGCACGCGACGCAGCGCATTGCCGAGCGTCAGGCCAAAGCC
GCGTTCCAGCGGCTCAGCGACGACGGTTGCATGCGTGCGGGAGCCGTGGGTGATGAAATCCACCTTGTTC
GGCTTGATCAGTTCCTGCCAGTTCTTCTGGATCA
Protein Sequence 
>gi|23502086|ref|NP_698213.1| DNA-directed RNA polymerase subunit alpha [Brucella suis 1330] MIQKNWQELIKPNKVDFITHGSRTHATVVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAVQIDGVLHEFSSIPGVREDVTDIVLNIKEIAIRMEGEGPKRMVVRKEGPGVVTAGDIQTVGDVEILNPEHVICTLDEGAEIRMEFTVNTGKGYVPADRNRAEDAPIGLIPVDSLYSPVRKVSYKIENTREGQVLDYDKLTLNIETNGSVTGEDAVAYAARILQDQLSIFVNFEEPQKEAPQEQVAELAFNPALLKKVDELELSVRSANCLKTDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEIPAWPPENIEDLAKRYEDQY
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates(UniProt: Q8G094).

CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)(UniProt: Q8G094).

SUBUNIT: Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity)(UniProt: Q8G094).

DOMAIN: The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements (By similarity)(UniProt: Q8G094).

SIMILARITY: Belongs to the RNA polymerase alpha chain family(UniProt: Q8G094).

MUTATION: The rpoA gene codes for the essential alpha-subunit of the RNA polymerase. B. melitensis rpoA mutant was found by signature-tagged mutagenesis from a mouse infection model. This disruption leaves a partially functional protein, impaired for the activation of virB transcription, as demonstrated by the absence of induction of the virB promoter in the Tn5::rpoA background. RpoA is involved in virB regulation in vitro. The mutant (Tn5::rpoA) was more resistant to oxidative stress (Lestrate et al., 2003).
COG COG0202K, under K: Transcription
References
Lestrate et al., 2003: Lestrate P, Dricot A, Delrue RM, Lambert C, Martinelli V, De Bolle X, Letesson JJ, Tibor A. Attenuated signature-tagged mutagenesis mutants of Brucella melitensis identified during the acute phase of infection in mice. Infection and immunity. 2003; 71(12); 7053-7060. [PubMed: 14638795].