Victors

lon from Brucella suis 1330
Victor ID	1075
Gene Name	lon from Brucella suis 1330
Sequence Strain (Species/Organism)	Brucella suis 1330
NCBI Gene ID	1166782
NCBI Protein GI	23501984
Locus Tag	BR1106
Protein Accession	NP_698111.1
Other Database IDs	UniProtKB-ID: LON_BRUSU UniRef100: UniRef100_Q8G0I7 UniRef90: UniRef90_Q2YPX3 UniRef50: UniRef50_A8HYF7 UniParc: UPI00000DD4C5 EMBL: AE014291 EMBL-CDS: AAN30026.1 RefSeq_NT: NC_004310.3 MEROPS: S16.001 GenomeReviews: AE014291_GR KEGG: bms:BR1106 NMPDR: fig\|204722.1.peg.1068 TIGR: BR1106 HOGENOM: HBG566281 OMA: IQKMSKV ProtClustDB: CLSK864999 BioCyc: BSUI204722:BR_1106-MONOMER
Taxonomy ID	204722
Chromosome No	I
Gene Starting Position	1081107
Gene Ending Position	1083545
Gene Strand (Orientation)	-
Protein Name	ATP-dependent protease La
DNA Sequence	>gi\|56968325:1081107-1083545 Brucella suis 1330 chromosome I, complete sequence CTTAATGGGCCAGCGAAGCCCCTGCTTCATCCTCCACCGGAGGCACGGCAGTGGGATTCTCCTGCTCGGT CCATTCAATCGGTTCAGGCTGGCGCACGAGCGTGTGCTTCAGCACTTCACCGACGCGGGATACCGGAACG ATCTCAAGATTGTTCTTCACATTGTCCGGGATTTCCGCCAGATCCTTGGCGTTCTCTTCCGGGATCAGAA CCTTCTTGATACCGCCGCGCAAGGCCGCAAGCAGCTTTTCCTTCAACCCGCCGATTGGCAGAACCCGACC GCGCAACGTGACTTCACCCGTCATGGCGATGTCCTTGCGAACGGGAATACCCGTCAGCACGGAGACGATG GCCGTAACCATGGCGATACCGGCAGAAGGACCATCCTTCGGCGTCGCGCCTTCCGGCACGTGCACGTGGA TATCGCGCTTGTCGAACAGCGGAGGCTCGATGCCGAAATCGATCGCGCGCGAGCGGACATAGGATGCCGC GGCCGAAATCGATTCCTTCATCACGTCACGGAGATTACCCGTAACCGTCATGCGGCCCTTACCCGGCATC ATGACGCCTTCGATGGTCAAAAGCTCACCGCCGACTTCGGTCCAGGCAAGGCCAGTCACGACACCCACCT GATCTTCACCGTCGATCTGACCGAAGCGGAACTTCTCCACACCAAGGTAATCGGAGAGGTTCTTGTCGGT AATCTTTACCGACTTCTTCTTCGTCTTCAGGATTTCCGTCACGGCCTTGCGCGCAAGGGTCATCACCTCG CGTTCAAGGCTACGCACGCCCGCTTCCCGCGTATAATGGCGGATAACGTTGCGCAGCGCATCTTCCGTAA CCGAAAACTCCTTCGGTTGCAGGGCATGGTCCTTGATGGCCTTCGGCAACAGGTGCCGCTTGGCGATCTC CAGCTTTTCGTCTTCCGTGTAACCGGCGATACGGATGATCTCCATACGATCCAGAAGTGGACCGGGAATA TTCATCGTATTGGCGGTCGTCACGAACATGACGTTCGATAGATCATACTCAACTTCAAGGTAGTGATCCA TGAAGGTCGCGTTCTGTTCCGGGTCCAGCACCTCCAGCATGGCCGAAGACGGATCGCCGCGGAAATCCTG GCCCATCTTGTCGATTTCATCGAGCAGGAAAAGCGGATTGGACTTCTTCGCCTTCTTCATCGACTGGATG ACCTTGCCGGGCATCGAGCCGATATAGGTGCGGCGATGACCGCGGATTTCAGCCTCGTCGCGTACGCCGC CAAGCGACATGCGGACATATTCGCGGCCCGTTGCCTTGGCAATCGAGCGCGCAAGCGAGGTCTTGCCGAC GCCGGGAGGGCCAACGAGGCAGAGGATCGGACCCTTGATCTTGGTCGAACGGGCCTGCACCGCCAGATAT CCGACGATGCGTTCCTTGACCTTGCCAAGGCCGAAATGCTCCGCATCCAGCACTTCCTGCGCAAAGTTCA GATCCTGCTTCACCTTCGACTTCTTGCCCCATGGAATGGAGAGCAACCAGTCGAGATAATTGCGAACCAC CGTCGCTTCAGCGGACATCGGGCTCATGCTGCGCAGTTTCTTCAGCTCGGCCAGAGCTTTTTCGCGCGCT TCCTTGCTGAGCTTGGTCTTGGTGATGCGCTCTTCGATTTCGGCCACTTCATCACGGCCGTCCTCACTGT CGCCAAGCTCCTTCTGGATCGCCTTCATCTGCTCATTGAGATAATATTCGCGCTGCGTCTTCTCCATCTG GCGCTTGACGCGGCTGCGAATACGCTTCTCAACCTGTAGAACAGAAATTTCGGCTTCCATGAAGGAAAGG GCTTTCTCAAGGCGCTCGCGCACCGAAAGAACCGACAGCATTTCCTGCTTTTCAGGGATTTTGATCGCAA GGTGCGAGGCAACCGTATCGGCAAGCTTGGAATAATCGTCGATCTGGCTGGCCGCGCCAACCACTTCCGG CGAAATCTTCTTGTTCAGCTTGACGTAATTTTCAAAGTCGGAAACCACCGAGCGGGCCAGTGCCTCGATC TCGACAGCATCTTCCTCCGGCTCCTGCAGAGCCGCAGCATAAGCCTCGTGATAATCTTCACGATCGGTAA ACTTGGAAATTTTGGCGCGTGCCGTGCCTTCGACCAGCACCTTGACGGTGCCGTCCGGCAGTTTCAGAAG CTGCAACACATTGGCGATCGTACCAATCTCATAGATCGCGTCCGGCGCCGGATCGTCATCGGCAGCGTTC TTCTGGGTGGCAAGCAATATCTGCTTGTCAACGCCCATCACTTCTTCAAGCGCGCGAATGGATTTTTCGC GGCCCACAAAAAGCGGGACGATCATATGGGGAAAGACGACGATGTCACGCAGCGGCAAGACAGCATAGAG CCCGTCCGCACCACCCGTTTCAGAACCACCAACCGGTGTTTTCTGTTCAATACCCGTCA
Protein Sequence	>gi\|23501984\|ref\|NP_698111.1\| ATP-dependent protease La [Brucella suis 1330] MTGIEQKTPVGGSETGGADGLYAVLPLRDIVVFPHMIVPLFVGREKSIRALEEVMGVDKQILLATQKNAADDDPAPDAIYEIGTIANVLQLLKLPDGTVKVLVEGTARAKISKFTDREDYHEAYAAALQEPEEDAVEIEALARSVVSDFENYVKLNKKISPEVVGAASQIDDYSKLADTVASHLAIKIPEKQEMLSVLSVRERLEKALSFMEAEISVLQVEKRIRSRVKRQMEKTQREYYLNEQMKAIQKELGDSEDGRDEVAEIEERITKTKLSKEAREKALAELKKLRSMSPMSAEATVVRNYLDWLLSIPWGKKSKVKQDLNFAQEVLDAEHFGLGKVKERIVGYLAVQARSTKIKGPILCLVGPPGVGKTSLARSIAKATGREYVRMSLGGVRDEAEIRGHRRTYIGSMPGKVIQSMKKAKKSNPLFLLDEIDKMGQDFRGDPSSAMLEVLDPEQNATFMDHYLEVEYDLSNVMFVTTANTMNIPGPLLDRMEIIRIAGYTEDEKLEIAKRHLLPKAIKDHALQPKEFSVTEDALRNVIRHYTREAGVRSLEREVMTLARKAVTEILKTKKKSVKITDKNLSDYLGVEKFRFGQIDGEDQVGVVTGLAWTEVGGELLTIEGVMMPGKGRMTVTGNLRDVMKESISAAASYVRSRAIDFGIEPPLFDKRDIHVHVPEGATPKDGPSAGIAMVTAIVSVLTGIPVRKDIAMTGEVTLRGRVLPIGGLKEKLLAALRGGIKKVLIPEENAKDLAEIPDNVKNNLEIVPVSRVGEVLKHTLVRQPEPIEWTEQENPTAVPPVEDEAGASLAH
Molecule Role	Virulence factor
Molecule Role Annotation	FUNCTION: Degrades short-lived regulatory and abnormal proteins in presence of ATP. Hydrolyzes two ATPs for each peptide bond cleaved in the protein substrate (By similarity)(UniProt: Q8G0I7). CATALYTIC ACTIVITY: Hydrolysis of proteins in presence of ATP(UniProt: Q8G0I7). SUBUNIT: Homotetramer (By similarity)(UniProt: Q8G0I7). SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: Q8G0I7). SIMILARITY: Belongs to the peptidase S16 family(UniProt: Q8G0I7). SIMILARITY: Contains 1 Lon domain(UniProt: Q8G0I7). MUTATION: In contrast to the parent strain, the Brucella abortus lon mutant, was impaired in its capacity to form isolated colonies on solid medium at 41 degrees C and displayed an increased sensitivity to killing by puromycin and H₂O2. Brucella abortus Lon homologue functions as a stress response protease that is required for wild-type virulence during the initial stages of infection in the mouse model, but is not essential for the establishment and maintenance of chronic infection in this host (Robertson et al., 2000). Both single lon or clpA mutations had comparable effects on growth inhibition, suggesting that the concerned proteases Lon and ClpAP both degrade a number of specific proteins, but are also both involved in general degradation of abnormal proteins. Compared to the single mutants, the double mutant lon clpA was highly sensitive to canavanine. One possible explanation for this observation is that both proteases can substitute for each other to a large extent during bacterial growth. Hence, simultaneous inactivation or decrease in activation of both proteases, either by direct mutation or by elimination of the regulatory component ClpA, strongly increased growth inhibition (Robertson et al., 2000).
COG	COG0466O, under O: Posttranslational modification, protein turnover, chaperones
References	*Robertson et al., 2000:* Robertson GT, Kovach ME, Allen CA, Ficht TA, Roop RM 2nd. The Brucella abortus Lon functions as a generalized stress response protease and is required for wild-type virulence in BALB/c mice. Molecular microbiology. 2000; 35(3); 577-588. [PubMed: 10672180].


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lon from Brucella suis 1330