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purL from Brucella suis 1330

Victor ID 1063
Gene Name purL from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1166505
NCBI Protein GI 23501724
Locus Tag BR0837
Protein Accession NP_697851.1
Other Database IDs UniProtKB-ID: PURL_BRUSU
UniRef100: UniRef100_A9MAL4
UniRef90: UniRef90_A9MAL4
UniRef50: UniRef50_Q8UEB0
UniParc: UPI00000DD3FB
EMBL: AE014291
EMBL-CDS: AAN29766.1
RefSeq_NT: NC_004310.3
GenomeReviews: AE014291_GR
KEGG: bms:BR0837
NMPDR: fig|204722.1.peg.808
TIGR: BR0837
HOGENOM: HBG311214
OMA: YGNSFGV
ProtClustDB: PRK01213
BioCyc: BSUI204722:BR_0837-MONOMER
Taxonomy ID 204722
Chromosome No I
Gene Starting Position 813718
Gene Ending Position 815940
Gene Strand (Orientation) -
Protein Name phosphoribosylformylglycinamidine synthase II
DNA Sequence 
>gi|56968325:813718-815940 Brucella suis 1330 chromosome I, complete sequence
ATCAGTTATTGCCGGTCAGTTCGCCGTTCATGAAGTTCGGGAACCAACCTTCATAAGCCTGCGTCAGATC
GGCCACGCTGACATCGACATTCTTGCTGATTTTCAGCCGGTCGCCGCCAACTGTACCCAAAATGCGGAAT
GGAACGCCTGCGCCTTCCGCGTTAAGCGCGATGAGCTTTGCCATTTCAGGCGTGGCGGAAATAACATAGC
GTGCCTGATCTTCACCGAAAAGCAGGGCGTGCGGCAGGCCGTCACCAGCGTCCAGCGTCGCGCCTTTGCC
GGACTTGATCGCCATTTCGGCAACGGCAATCGCCAGACCGCCATCGGAAAGATCATGGCAGGCCGTCACC
TGACCGTTGCGAATGGCAGAACGGACAAATTCGCCATTGCGCTTTTCAAGCGCCAGATCGACAAAAGGCG
CAGGACCGTCCGCGCGGTCGAAAAGGTCACGCAGATAGACCGACTGGCCGAGATGCGTTCCGTCACCACC
GAGCAGAACCAGCGTATCGCCATCCTGCATGCCGCCGATCTTCGCCATTTGCGACCAGTCGGGAAGGAGG
CCAACACCCGCAATCGTTGGCGTTGGCAGAATGGCCTGGCCGTTGGTCTCATTGTAAAGCGACACATTGC
CGGACACGATCGGGAAGTCGAGTGCGCGGCAGGCCTCGCCAATGCCTTCGATAGCCTTGACGAGCTGGCC
CATGATTTCCGGCTTTTCGGGATTGCCGAAGTTCAGATTATCGGTCGAGGCCAGCGGCTCCGCGCCGGTC
GCCGTGATGTTGCGCCAGCATTCGGCAACGGCCTGCTTGCCGCCTTCAAACGGATCGGCTTCGCAATAGC
GCGGCGTCACGTCGGAAGAGAAGGCGAGAGCCTTGGTTTCATGGCCTTCGACACGGATTACACCGGCGTC
GCCGCCCGGAACCTGCAAGGAATTGCCCTGAATGAGCGTGTCGTACTGTTCATAGACCCAGCGGCGCGAG
GAAAGATCGGGCGAGCCGATCAGCTTCAGCAGGGCTGCGGAATAGTCTTCCACCTGCGGCACATTGCTGG
CAGGCAGCGGCGCATGCTTGCCCGGCTCCATCCACGGGCGATCATATTCCGGCGCTTCGTCGCCCAGATC
CTTGATCGGCAGATTGGCGACCTCTTCCCCCTGATGGATGACGCGGAAACGAAGATCGTCCGTGGTCTTG
CCGACAATCGCGAAATCAAGGCCCCATTTGCGGAAGATAGCCTGCGCTTCTGCTTCCTTTTCCGGCTTCA
GCACCATGAGCATGCGCTCCTGGCTTTCCGAAAGCATCATTTCATAGGCGGTCATGTTCTCTTCGCGAAC
GGGAACATGGTCGAGAATGAGTTCGATGCCGAGATCGCCCTTGGCACCCATCTCGACCGCCGAGCAGGTG
AGGCCGGCAGCGCCCATGTCCTGAATGGCGATGACCGCACCGGAAGCCATGAGTTCAAGGCAGGCTTCAA
GCAGGCATTTTTCGGTGAAAGGATCGCCCACCTGAACGGTGGGGCGCTTTTCCTCGATCGACTCGTCGAA
TTCAGCCGATGCCATTGTTGCACCGCCAACACCATCGCGGCCAGTCTTTGCACCCAGATAGACGACCGGC
AGGCCGACGCCCTTGGCTTCCGACAGGAAAATGCCATCATGCCGGGCAAGACCGGCAGCAAAGGCATTGA
CGAGGATATTGCCGTTATAGCGCTTGTCGAAATTCACCTCGCCACCGACAGTCGGCACGCCGAAGGCATT
GCCATAGCCGCCGACGCCGGAAACAACGCCCGAGACGAGGTGGCGGGTTTTTGGATGGTCGGGTTCGCCA
AAGCGCAGCGCATTCATTGCCGCAACGGGGCGCGCGCCCATGGTGAACACGTCGCGCAAGATTCCGCCCA
CGCCGGTCGCGGCACCCTGATAAGGCTCGATATAGGAGGGGTGATTGTGGCTTTCCATCTTGAAGACGAC
GCAATCGCCATCGCCAATATCGACCACGCCTGCATTTTCACCGGGGCCTTGAATGACGCGCGGTCCGCTG
GTCGGAAGTGTGCGTAGCCATTTCTTGGAGGACTTGTAGGAGCAGTGCTCATTCCACATGGCGGAGAAAA
TGCCGAGTTCGGTAAAAGTCGGCTCGCGCCCGATCAGCTCAAGAATGCGCTGATACTCGTCCGGCTTAAG
ACCATGCGCTTCGATCAGTTCCGGCGTGATGTCTCGCGTATTGGAAATAGTCA
Protein Sequence 
>gi|23501724|ref|NP_697851.1| phosphoribosylformylglycinamidine synthase II [Brucella suis 1330] MTISNTRDITPELIEAHGLKPDEYQRILELIGREPTFTELGIFSAMWNEHCSYKSSKKWLRTLPTSGPRVIQGPGENAGVVDIGDGDCVVFKMESHNHPSYIEPYQGAATGVGGILRDVFTMGARPVAAMNALRFGEPDHPKTRHLVSGVVSGVGGYGNAFGVPTVGGEVNFDKRYNGNILVNAFAAGLARHDGIFLSEAKGVGLPVVYLGAKTGRDGVGGATMASAEFDESIEEKRPTVQVGDPFTEKCLLEACLELMASGAVIAIQDMGAAGLTCSAVEMGAKGDLGIELILDHVPVREENMTAYEMMLSESQERMLMVLKPEKEAEAQAIFRKWGLDFAIVGKTTDDLRFRVIHQGEEVANLPIKDLGDEAPEYDRPWMEPGKHAPLPASNVPQVEDYSAALLKLIGSPDLSSRRWVYEQYDTLIQGNSLQVPGGDAGVIRVEGHETKALAFSSDVTPRYCEADPFEGGKQAVAECWRNITATGAEPLASTDNLNFGNPEKPEIMGQLVKAIEGIGEACRALDFPIVSGNVSLYNETNGQAILPTPTIAGVGLLPDWSQMAKIGGMQDGDTLVLLGGDGTHLGQSVYLRDLFDRADGPAPFVDLALEKRNGEFVRSAIRNGQVTACHDLSDGGLAIAVAEMAIKSGKGATLDAGDGLPHALLFGEDQARYVISATPEMAKLIALNAEGAGVPFRILGTVGGDRLKISKNVDVSVADLTQAYEGWFPNFMNGELTGNN
Molecule Role Virulence factor
Molecule Role Annotation CATALYTIC ACTIVITY: ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H(2)O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate(UniProt: Q8G183).

PATHWAY: Nucleotide biosynthesis; IMP biosynthesis; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1(UniProt: Q8G183).

PATHWAY: Context: Purine biosynthesis(UniProt: Q8G183).

SUBUNIT: Heterodimer of two subunits, purQ and purL(UniProt: Q8G183).

SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: Q8G183).

SIMILARITY: Belongs to the FGAMS family(UniProt: Q8G183).

MUTATION: Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALBc mouse model. It confirms the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages (Alcantara et al., 2004). Studies with B melitensis purE and B abortus purL mutants, which are purine auxotrophs, and B suis aroC mutants, which can not synthesize aromatic amino acids, indicate that the brucellae also face signicant nutrient limitation during their prolonged residence in host macrophages (Alcantara et al., 2004).
COG COG0046F, under F: Nucleotide transport and metabolism
References
Alcantara et al., 2004: Alcantara RB, Read RD, Valderas MW, Brown TD, Roop RM 2nd. Intact purine biosynthesis pathways are required for wild-type virulence of Brucella abortus 2308 in the BALB/c mouse model. Infection and immunity. 2004; 72(8); 4911-4917. [PubMed: 15271960].