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purD from Brucella suis 1330

Victor ID 1037
Gene Name purD from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1166075
NCBI Protein GI 23501317
Locus Tag BR0414
Protein Accession NP_697444.1
Other Database IDs UniProtKB-ID: PUR2_BRUSU
UniRef100: UniRef100_Q8G2B1
UniRef90: UniRef90_Q8YFK1
UniRef50: UniRef50_P52420
UniParc: UPI00000DD2B8
EMBL: AE014291
EMBL-CDS: AAN29359.1
RefSeq_NT: NC_004310.3
GenomeReviews: AE014291_GR
KEGG: bms:BR0414
NMPDR: fig|204722.1.peg.401
TIGR: BR0414
HOGENOM: HBG404060
OMA: GVPIVIK
ProtClustDB: PRK00885
BioCyc: BSUI204722:BR_0414-MONOMER
Taxonomy ID 204722
Chromosome No I
Gene Starting Position 418973
Gene Ending Position 420256
Gene Strand (Orientation) -
Protein Name phosphoribosylamine--glycine ligase
DNA Sequence 
>gi|56968325:418973-420256 Brucella suis 1330 chromosome I, complete sequence
ATCAGCGATTAGCCTTCTCACGCTCCACGGCGCGCCAGCCGATATCAGAACGGTAAAAACCTTCCGGCCA
GTCGATGAGCTTCAGCGCCTCATAGGCACGCGCCTGCGCTTGCGCTACGGTTGCCGCCGTAGCGGTGATG
TTGAGGACGCGCCCGCCGCCGGCGACGATGGCGCCGTCCTTCAGCGCCGTGCCCGCATGGAACAGTTTTA
CGCCATCAATGCTTTCCAGCTTTTCGAGGTCGCGGATGACGCTGCCCTTCTTCACATTGGCCGGGTAGCC
TTCGGCGGCCATCACTACGGTCAGCGCCGGTTCGTCTTTCCATTCAAGTGAAACCTCATCAAGTCTGCCG
TCAACGGCAGCATTGATGAGGGCAAGCAGATCGCTGTTGAGGCGCATCATCAATACCTGGCATTCCGGAT
CGCCGAAACGGGTATTATATTCGATGAGCTTCGGGCCATCGCTGGTAATCATCAGCCCGGCAAAGAGAAT
GCCGGAAAAGGGTGCGCCGATTTCCGCCATGCCGCGCATGGTGGGTTCAATCAGTTCGCGCATGGTGCGC
GCCACCATTTCCGGTGTCATCACCGGGGCGGGCGCATAGGCGCCCATGCCGCCGGTATTCGGGCCGGTAT
CGCCATCGCCGACGCGCTTGTGATCCTGCGCGGAGCCAAGCGGCAATGCCGTCTTGCCATCGCAGATGCA
GAAGAAGCTTGCTTCCTCACCGTCAAGAAATTCCTCGACCACCACTTCCGCGCCTGCCGCACCGAAAGCG
CCCTCAAAGCAGGAATCGACCGCATCAAGTGCTTCCTGAAGCGTCATGGCGACAACCACGCCCTTGCCTG
CGGCAAGCCCATCGGCCTTGACCACGATGGGCGCGCCCTGCTGGCGGATATAGGCTTTCGCCTTTGGCGC
ATTGTTGAAACGGCCATAAGCGCCGGTCGGGATATTGAAGCGCGCGCAGAGATCCTTGGTGAAGCCCTTC
GAGCCTTCAAGCTGGGCTGCGGCCCTGGACGGGCCGAAGACGCGGATGCCCTCAGCGCGCATTTCATCGG
CAAGCCCGGTGACAAGCGGCGCTTCCGGCCCCACGACCACGAGGTCGATATGCTTTTCCTTGGCAAAGGC
AATCAGCGCGGCATGGTCGTCCACGCCGATATCGGCAAGCTCCGCTACAGCGGCGATACCGGGATTGCCG
GGCGCGCAATATAGTTTTTCAAGCAGGGGCGAGGCGGCAAGCTTCCATGCCAAGGCGTGCTCGCGACCAC
CGGAACCGATCAACAGGACTTTCA
Protein Sequence 
>gi|23501317|ref|NP_697444.1| phosphoribosylamine--glycine ligase [Brucella suis 1330] MKVLLIGSGGREHALAWKLAASPLLEKLYCAPGNPGIAAVAELADIGVDDHAALIAFAKEKHIDLVVVGPEAPLVTGLADEMRAEGIRVFGPSRAAAQLEGSKGFTKDLCARFNIPTGAYGRFNNAPKAKAYIRQQGAPIVVKADGLAAGKGVVVAMTLQEALDAVDSCFEGAFGAAGAEVVVEEFLDGEEASFFCICDGKTALPLGSAQDHKRVGDGDTGPNTGGMGAYAPAPVMTPEMVARTMRELIEPTMRGMAEIGAPFSGILFAGLMITSDGPKLIEYNTRFGDPECQVLMMRLNSDLLALINAAVDGRLDEVSLEWKDEPALTVVMAAEGYPANVKKGSVIRDLEKLESIDGVKLFHAGTALKDGAIVAGGGRVLNITATAATVAQAQARAYEALKLIDWPEGFYRSDIGWRAVEREKANR
Molecule Role Virulence factor
Molecule Role Annotation CATALYTIC ACTIVITY: ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide(UniProt: Q8G2B1).

PATHWAY: Nucleotide biosynthesis; IMP biosynthesis; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2(UniProt: Q8G2B1).

PATHWAY: Context: Purine biosynthesis(UniProt: Q8G2B1).

SIMILARITY: Belongs to the GARS family(UniProt: Q8G2B1).

SIMILARITY: Contains 1 ATP-grasp domain(UniProt: Q8G2B1).

MUTATION: Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALBc mouse model. It confirms the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages (Alcantara et al., 2004).

Like the purE mutant, a purD::Tn10 mutant has reduced survival in murine macrophages and reduced virulence in mice (Alcantara et al., 2004).
COG COG0151F, under F: Nucleotide transport and metabolism
References
Alcantara et al., 2004: Alcantara RB, Read RD, Valderas MW, Brown TD, Roop RM 2nd. Intact purine biosynthesis pathways are required for wild-type virulence of Brucella abortus 2308 in the BALB/c mouse model. Infection and immunity. 2004; 72(8); 4911-4917. [PubMed: 15271960].