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ilvD from Brucella suis 1330

Victor ID 1026
Gene Name ilvD from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1165756
NCBI Protein GI 23501013
Locus Tag BR0099
Protein Accession NP_697140.1
Other Database IDs UniProtKB-ID: ILVD_BRUSU
UniRef100: UniRef100_A9M6V2
UniRef90: UniRef90_B2S7Z5
UniRef50: UniRef50_Q5F8G6
UniParc: UPI00000DD1C5
EMBL: AE014291
EMBL-CDS: AAN29055.1
RefSeq_NT: NC_004310.3
GenomeReviews: AE014291_GR
KEGG: bms:BR0099
NMPDR: fig|204722.1.peg.97
TIGR: BR0099
HOGENOM: HBG671001
OMA: KVPCLSK
ProtClustDB: PRK12448
BioCyc: BSUI204722:BR_0099-MONOMER
Taxonomy ID 204722
Chromosome No I
Gene Starting Position 103312
Gene Ending Position 105147
Gene Strand (Orientation) -
Protein Name dihydroxy-acid dehydratase
DNA Sequence 
>gi|56968325:103312-105147 Brucella suis 1330 chromosome I, complete sequence
ATCAATCCGGCAGCTTTCGCACCGCGCCCCTGGCGGCACTTGTCGCCATTGCCGCATAGGCTTTCAGCGC
AGTGGAGATTTTACGCTTGCGTTCCTCTGCCGGTTTCCAGCCTGCTGCATCCTGCTCGGCGCGGCGTTCG
GCAAGTGTGGCGTCGTCAACGGCAAGGTGGATCTTGCGGTTCGGAATGTCGATATCGATGATATCGCCTT
CGCGCACCAGACCGATCGTGCCGCCTTCGGCTGCTTCCGGCGAAACATGGCCGATGGAAAGGCCCGATGA
ACCGCCCGAGAAACGTCCGTCGGTGATAAGTGCGCAGGCTTTGCCGAAGCCCTTCGACTTCAGATAGCTG
GTCGGGTAGAGCATTTCCTGCATTCCGGGGCCGCCGCGCGGGCCTTCATAGCGGATCAGCACGATGTCGC
CCGGCTTGATCTTGCCGTTCAAAATGCCAAGCACAGCCGAATCCTGGCTTTCAAAAATGCGGGCCGGGCC
GGAGAATTTCAGGATGGAATCGTCCACGCCTGCCGTCTTCACGATGCAGCCGTCTTCCGCCAGATTGCCG
TAAAGCACGGCCAAGCCGCCATCCTGGCTGAAAGCGTGTTCCTTTGAGCGGATGACGCCTTTTTCGCGGT
CGGTGTCCACCTTGTCGAAGCGCCGCTCCTGGCTGAAAGCCACCTGCGTCGGCACGCCGCCGGGAGCGGC
GGAATAGAATTTGTGCACCATTTCGCTGTTTGTGCGCGTTACATCCCAATGATCAAGCGCCTTAGCGAGC
GTTTCGCTGTGCACGGTCGGGATGCTGGTGGTCAGCAGGCCCGCATTGTCGAGCTGTCCGAGAATGCCCA
TGATGCCGCCGGCATGATGCACGTCTTCCATGTGAACCGACGACACGGCGGGCGCCACCTTGCACAGCAC
CGGCACGCGGCGCGACAGGCGGTCTATATCCGCCATGGTGAAATCGATTTCCGCTTCCTGCGCAGCCGCG
AGCAGATGCAGAACCGTATTGGTCGAACCGCCCATGGCGATATCGAGCGTCATCGCGTTCTCGAAAGCCG
AGAAAGTGGCGATGGAGCGCGGCAGCACACTTTCGTCATCCTGCTCGTAATAGCGGCGGGCGAGATCGAC
GATCAGGTGGCCAGCTTCGACGAAGAGGCGCTTGCGGTCTGCATGGGTGGCGAGCGTCGAACCATTGCCG
GGCAGCGACAGGCCGAGCGCTTCGGTAAGGCAATTCATCGAATTGGCCGTGAACATGCCCGAACACGACC
CACAGGTCGGGCAGGCGGAACGCTCAATCGCCTTCACCTGCTCGTCGGTATAATGGTCGTCGGCCGCCGC
CACCATGGCGTCCACGAGGTCGAGCTTCTTGACAGAATCCTCCCACACGACCTTGCCTGCTTCCATCGGC
CCACCGGAGACGAACACGACCGGAATATTGAGACGCAGCGCCGCCATCAGCATGCCGGGCGTGATCTTGT
CGCAATTGGAGATGCAGACCATCGCATCGGCGCAATGCGCATTGACCATATATTCGACCGAGTCGGCGAT
GAGCTCACGGGAGGGGAGCGAATAGAGCATGCCGTCATGGCCCATGGCGATGCCGTCATCGACCGCAATG
GTATTGAATTCCTTGGCGACGCCGCCCGCACTTTCGATTTCGCGCGCGACGAGCTGGCCGAGATCCTTCA
GATGCACATGGCCGGGCACGAACTGGGTGAACGAGTTCACCACGGCGATAATCGGCTTGCCGAAATCCTC
ATCCTTCATGCCGGTGGCGCGCCATAGGCCGCGCGCGCCGGCCATATTGCGGCCATGGGTGGTTGTACGC
GAACGATAAGGAGGCA
Protein Sequence 
>gi|23501013|ref|NP_697140.1| dihydroxy-acid dehydratase [Brucella suis 1330] MPPYRSRTTTHGRNMAGARGLWRATGMKDEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIESAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAALRLNIPVVFVSGGPMEAGKVVWEDSVKKLDLVDAMVAAADDHYTDEQVKAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHADRKRLFVEAGHLIVDLARRYYEQDDESVLPRSIATFSAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMADIDRLSRRVPVLCKVAPAVSSVHMEDVHHAGGIMGILGQLDNAGLLTTSIPTVHSETLAKALDHWDVTRTNSEMVHKFYSAAPGGVPTQVAFSQERRFDKVDTDREKGVIRSKEHAFSQDGGLAVLYGNLAEDGCIVKTAGVDDSILKFSGPARIFESQDSAVLGILNGKIKPGDIVLIRYEGPRGGPGMQEMLYPTSYLKSKGFGKACALITDGRFSGGSSGLSIGHVSPEAAEGGTIGLVREGDIIDIDIPNRKIHLAVDDATLAERRAEQDAAGWKPAEERKRKISTALKAYAAMATSAARGAVRKLPD
Molecule Role Virulence factor
Molecule Role Annotation CATALYTIC ACTIVITY: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H(2)O(UniProt: Q8G353).

COFACTOR: Binds 1 4Fe-4S cluster (Potential)(UniProt: Q8G353).

PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3(UniProt: Q8G353).

PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3(UniProt: Q8G353).

SIMILARITY: Belongs to the ilvD/edd family(UniProt: Q8G353).

MUTATION: Of those B abortus mutants with mini-Tn5 insertions in genes predicted to be involved in amino acid biosynthesis and transport, only the ilvD mutant, displayed attenuation in both macrophages and mice. The othre two amino acid biosynthesis mutants [trpB::miniTn5 and pheA::miniTn5] displayed wild-type virulence in mice but attenuated inside macrophages. The studies with B abortus ilvD, trpB, and pheA mutants suggest that tryptophan and phenylalanine are available to the brucellae in their intracellular niche but that other amino acids (eg, leucine, isoleucine, or valine) are not (Alcantara et al., 2004).
COG COG0129EG, under E: Amino acid transport and metabolism; G: Carbohydrate transport and metabolism
References
Alcantara et al., 2004: Alcantara RB, Read RD, Valderas MW, Brown TD, Roop RM 2nd. Intact purine biosynthesis pathways are required for wild-type virulence of Brucella abortus 2308 in the BALB/c mouse model. Infection and immunity. 2004; 72(8); 4911-4917. [PubMed: 15271960].