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eryC from Brucella suis 1330

Victor ID 1012
Gene Name eryC from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1165309
NCBI Protein GI 23500594
Locus Tag BRA0866
Protein Accession NP_700034.1
Other Database IDs UniProtKB-ID: Q8FVH5_BRUSU
UniRef100: UniRef100_D0BG56
UniRef90: UniRef90_A6X313
UniRef50: UniRef50_A6X312
UniParc: UPI00000DDAA4
EMBL: AE014292
EMBL-CDS: AAN34039.1
RefSeq_NT: NC_004311.2
GenomeReviews: AE014292_GR
KEGG: bms:BRA0866
NMPDR: fig|204722.1.peg.2943
TIGR: BRA0866
HOGENOM: HBG300233
OMA: HFGHPDA
ProtClustDB: CLSK864691
BioCyc: BSUI204722:BR_A0866-MONOMER
Taxonomy ID 204722
Chromosome No II
Gene Starting Position 851751
Gene Ending Position 852683
Gene Strand (Orientation) +
Protein Name D-erythrulose-1-phosphate dehydrogenase
DNA Sequence 
>gi|56968493:851751-852683 Brucella suis 1330 chromosome II, complete genome
TATGGCCCTGACGCTTTCGCTCAACACCAATCCGCTGGTCAACCGCTTTGCCGAGCCGGACGACCTGATC
GAAACGGTTGCCCGCGACCTGCGCCTGCGTGACCTCCAGCTTACCCATGAGTTCATCAATCCAAGCTGGC
AGGCCTCGACCATCCGCCGCCTCACCCGCGACATGGACAGGGCCTTGCAGCGCACCGGTGTCCGCGTCAC
CTCCGGCATGACCGGCCCCTATGGCCGCTTCAACCATTTTGGCCATCCCGACCGGGACGTGCGCCGCTAT
TATGTGGACTGGTTCAAGACCTTTGCCGATATTATCGGCGATCTTGGCGGCAAGTCCGTCGGTACGCAGT
TTGCAATCTTCACCTATAAGGATTTCGATGATCCGGCGCGCCGCGAAGAACTTATCAAGATCGCCATCGA
CTGCTGGGCCGAGGTGGCCGAACATGCGGCAGGTGCGGGCCTCGACTATGTGTTCTGGGAGCCGATGAGC
ATCGGGCGCGAATTTGGCGAGACGATTGCCGAATGCATGAAGCTTCAGGATCGGCTCACCGCCGCTAACA
TGGCGATCCCCATGTGGATGATGGCCGATATCGACCATGGTGACGTGACATCCGCTAACCCGGACGACTA
CGATCCTTACGCATGGGCCCGCACCGTGCCGAAAGTCTCGCCCATCATCCATATCAAGCAAAGCCTGATG
GACAAGGGCGGGCATCGTCCTTTCACAGCCGCGTTCAATGCCAAGGGCCGCATCCAGCCGGAACCGCTTT
TGAAAGCCTTTGCCGAAGGCGGCGCGGTGGATAATGAAATCTGCCTTGAACTTTCGTTCAAGGAGCGCGA
GCCGAACGACCGTGAAGTCATTCCACAGATTGCAGAAAGTGTGGCTTTCTGGGCGCCGCACATTGACACC
GGCGCTAAGGACTTGAAGATATA
Protein Sequence 
>gi|23500594|ref|NP_700034.1| D-erythrulose-1-phosphate dehydrogenase [Brucella suis 1330] MALTLSLNTNPLVNRFAEPDDLIETVARDLRLRDLQLTHEFINPSWQASTIRRLTRDMDRALQRTGVRVTSGMTGPYGRFNHFGHPDRDVRRYYVDWFKTFADIIGDLGGKSVGTQFAIFTYKDFDDPARREELIKIAIDCWAEVAEHAAGAGLDYVFWEPMSIGREFGETIAECMKLQDRLTAANMAIPMWMMADIDHGDVTSANPDDYDPYAWARTVPKVSPIIHIKQSLMDKGGHRPFTAAFNAKGRIQPEPLLKAFAEGGAVDNEICLELSFKEREPNDREVIPQIAESVAFWAPHIDTGAKDLKI
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: The eryC gene encodes for enzyme Derythrulose-1-phosphate dehydrogenase. The vaccine strain B abortus B19 is the only known B abortus isolate whose growth is inhibited by erythritol. The B abortus B19 strain is an eryCD double mutant. The defect in B19 was complemented in trans by plasmids containing the complete ery region and by plasmids with Tn1725 insertions in eryA, eryB and eryD. Plasmids with Tn1725 insertions in eryC were the only ones that failed to complement the Ery phenotype of B19 (Sangari et al., 2000).

Allelic exchange mutants in eryC of Brucella suis were erythritol sensitive in vitro with a MIC of 1 to 5 mM of erythritol. Their multiplication in macrophage-like cells was 50 to 90- fold reduced , but complementation of the mutant restored wild-type levels of intracellular multiplication and the capacity to use erythritol as a sole carbon source. In vivo, the eryC mutant colonized the spleens of infected BALBc mice to a significantly lower extent than the wild type and the complemented strain. Interestingly, eryC mutants that were in addition spontaneously erythritol tolerant nevertheless exhibited wild-type-like intramacrophagic and intramurine replication. In conclusion, erythritol was not an essential carbon source for the pathogen in the macrophage host cell but that the inactivation of the eryC gene significantly reduced the intramacrophagic and intramurine fitness of B suis (Sangari et al., 2000).
References
Sangari et al., 2000: Sangari FJ, Agüero J, García-Lobo JM. The genes for erythritol catabolism are organized as an inducible operon in Brucella abortus. Microbiology (Reading, England). 2000; 146 ( Pt 2); 487-495. [PubMed: 10708387].