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sodC from Brucella suis 1330

Victor ID 1003
Gene Name sodC from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1165145
NCBI Protein GI 23500443
Locus Tag BRA0703
Protein Accession NP_699883.1
Other Database IDs UniProtKB-ID: SODC_BRUSU
UniRef100: UniRef100_P66826
UniRef90: UniRef90_P66826
UniRef50: UniRef50_P0AGD2
UniParc: UPI0000058534
EMBL: AE014292
EMBL-CDS: AAN33888.1
RefSeq_NT: NC_004311.2
GenomeReviews: AE014292_GR
KEGG: bms:BRA0703
TIGR: BRA0703
HOGENOM: HBG609879
ProtClustDB: CLSK898879
BioCyc: BSUI204722:BR_A0703-MONOMER
Taxonomy ID 204722
Chromosome No II
Gene Starting Position 682571
Gene Ending Position 683092
Gene Strand (Orientation) -
Protein Name superoxide dismutase, Cu-Zn
DNA Sequence 
>gi|56968493:682571-683092 Brucella suis 1330 chromosome II, complete genome
GTTATTCGATCACGCCGCAGGCAAAACGGGCACCACCGCCACCAAGCGGCTCAGGCTTATCGGAATAATT
ATCCCCTCCGACATGGACCATCAAAGAACGCTGCTTGATTTCCGCCAATTTCTTGAGATGTGGAGCGACA
ACGGTTTCACTCACCTTGCCGTCAGCATTGGCGCTCAGGCGTGGCAAATCGCCCATATGTCCATCACCTT
CAGGCCCTAAATGGTGATGGGTATTACCCGGATCATAATGCCCGCCGGCAGCAAGAGCCGGTACGATCTT
GCCGTCTTTTTCTCCCGGAGCGCAGCTTGGATTTTCGTGAACATGAAAGCCATGATAGCCCGGCGTCAGC
TTTTCCATATTCACCTTGAAGTGCAGCCCGCCCGGGGCTTCGGAAATGACCACGGTGCCAACTTCTTTAC
CCGGTCCGGTCGGCAGCGCCTCATACATTTTTACCGTCGTGCTTTCTGCGAAAGCCGGAAAAGCCATAAG
CACCATTGTCGATGCAATAAATAAGGACTTCA
Protein Sequence 
>gi|23500443|ref|NP_699883.1| superoxide dismutase, Cu-Zn [Brucella suis 1330] MKSLFIASTMVLMAFPAFAESTTVKMYEALPTGPGKEVGTVVISEAPGGLHFKVNMEKLTPGYHGFHVHENPSCAPGEKDGKIVPALAAGGHYDPGNTHHHLGPEGDGHMGDLPRLSANADGKVSETVVAPHLKKLAEIKQRSLMVHVGGDNYSDKPEPLGGGGARFACGVIE
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems (By similarity)(UniProt: P66827).

CATALYTIC ACTIVITY: 2 superoxide + 2 H(+) = O(2) + H(2)O(2)(UniProt: P66827).

COFACTOR: Binds 1 copper ion per subunit (By similarity)(UniProt: P66827).

COFACTOR: Binds 1 zinc ion per subunit (By similarity)(UniProt: P66827).

SUBUNIT: Homodimer (By similarity)(UniProt: P66827).

SUBCELLULAR LOCATION: Periplasmic (By similarity)(UniProt: P66827).

SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family(UniProt: P66827).

IMMUNOGENICITY: Induces antigen-specific Th1 immune response, as indicated by the specific induction of serum IgG2a, but not IgG1, antibodies and by the secretion of IFN-γ, but not IL-4, by the Cu/Zn SOD-stimulated splenocytes. Has been used for vaccine development (He et al., 2002; He et al., 2002; He et al., 2002).

MUTATION: An isogenic sodC mutant constructed from B abortus 2308 by gene replacement exhibited much greater susceptibility to killing by exogenous O(2)(-) than the parental 2308 strain, supporting a role for SodC in protecting this bacterium from O(2)(-) stress. The B abortus sodC mutant was much more sensitive to killing by cultured resident peritoneal macrophages from C57BL6J mice than 2308, and its attenuation in cultured murine macrophages was enhanced when these phagocytes were treated with gamma interferon. The attenuation of the B abortus sodC mutant in both resting and IFN-gamma -activated macrophages was alleviated in the presence of the NADPH oxidase inhibitor apocynin. Consistently, the B abortus sodC mutant also displayed significant attenuation in infected C57BL6J mice compared to the parental strain. These findings suggest that SodC protects B abortus 2308 from the respiratory burst of host macrophages (He et al., 2002).
COG COG2032P, under P: Inorganic ion transport and metabolism
References
He et al., 2002: He Y, Vemulapalli R, Schurig GG. Recombinant Ochrobactrum anthropi expressing Brucella abortus Cu,Zn superoxide dismutase protects mice against B. abortus infection only after switching of immune responses to Th1 type. Infection and immunity. 2002; 70(5); 2535-2543. [PubMed: 11953393].