Protegen ID	Gene Name	Sequence Strain (Species/Organism)	NCBI Gene ID	NCBI Nucleotide GI	NCBI Protein GI	Locus Tag	Genbank Accession	Protein Accession	Protein Name	Protein Annotation	Molecule Role	Molecule Role Annotation	COG	PMID
4897	cen1	Leishmania donovani			15488542				centrin		Virulence factor	MUTATION: A cen1 mutant is attenuated in mice and hamsters [Ref7328:Selvapandiyan et al., 2009].		19592661
5187	LdASS	Leishmania donovani	13389892		398015484	LDBPK_230300	FR799610	XP_003860931			Virulence factor	parasites expressing a mutant form of LdASS associated with a loss of in vitro activity had reduced virulence in vivo in BALB/c mice as demonstrated by a significant reduction in the parasite load in spleen and liver.[Ref7585:Lakhal-Naouar et al., 2012]; 		23094117
5188	Ldp27				386686540				mitochondrial P27		Virulence factor	the amastigote-specific protein p27 (Ldp27) is a component of an active cytochrome c oxidase complex in Leishmania donovani and that upon deletion of its gene the parasite had reduced virulence in vivo.   genetically modified live attenuated Ldp27(-/-) parasites are safe, induce protective immunity even in the absence of parasites, and can provide protection against homologous and heterologous Leishmania species.[Ref7586:Dey et al., 2010]; [Ref7587:Dey et al., 2013]		20497506  , 23338240
5189	SUB				282192977				subtilisin		Virulence factor	SUB-deficient Leishmania displayed reduced virulence in both hamster and murine infection models.; [Ref7588:Swenerton et al., 2010]		20675366
5190	OPB				259121914				oligopeptidase B		Virulence factor	OPB(-/-) parasites displayed decreased virulence in the murine footpad infection model.; [Ref7589:Swenerton et al., 2011]		20961853
5191	SPDSYN				10945607				spermidine synthase		Virulence factor	This Δspdsyn strain was auxotrophic for polyamines, required spermidine for growth in its insect vector form, and was adversely impacted in its ability to infect mice. These findings establish that SPDSYN, like ODC, is essential for maintaining a robust infection in mammals and indicate that pharmacologic inhibition of SPDSYN, and perhaps all components of the polyamine biosynthetic pathway, is a valid therapeutic strategy for the treatment of visceral and, potentially, other forms of leishmaniasis.[Ref7590:Gilroy et al., 2011]; 		21536795
5192	ornithine decarboxylase				159388				ornithine decarboxylase		Virulence factor	a Δodc L. donovani null mutant lacking ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine biosynthesis, was profoundly compromised in its ability to infect mice, indicating that ODC is essential for the infectious mammalian stage of the parasite and further validating the enzyme as a possible drug target.[Ref7590:Gilroy et al., 2011]; 		21536795
5193	CK1	Leishmania donovani	13388740		398016612	LDBPK_251640	FR799612	XP_003861494			Virulence factor	 parasites over expressing LdCK1.4 gave significantly higher infections of mouse peritoneal macrophages compared to wild-type parasites, 28.6% versus 6.3%, respectively (p = 0.0005). These results suggest that LdCK1.4 plays an important role in parasite survival and virulence. [Ref7591:Dan-Goor et al., 2013]; 		24260187
5199	LDBPK_070790	Leishmania donovani	13391812		398010638	LDBPK_070790	FR799594	XP_003858516			Virulence factor	We developed centrin gene deleted L. donovani parasites that displayed attenuated growth only in the amastigote stage and were found safe and efficacious against virulent challenge in the experimental animal models.[Ref7598:Selvapandiyan et al., 2014]; 		24837513