Protegen ID	Gene Name	Sequence Strain (Species/Organism)	NCBI Gene ID	NCBI Nucleotide GI	NCBI Protein GI	Locus Tag	Genbank Accession	Protein Accession	Protein Name	Protein Annotation	Molecule Role	Molecule Role Annotation	COG	PMID
4905	E(rns)	Classical swine fever virus - Brescia			130457				Genome polyprotein		Virulence factor	MUTATION: An N269A/Q mutation in the E(rns) protein created an attenuated mutant in swine [Ref7335:Sainz et al., 2008]. 		17904607
4906	E1	Classical swine fever virus - Brescia		325460	325461			AAA43843.1	protein precursor		Virulence factor	MUTATION: An N to A amino acid substitution was made in the N594 site of the E1 glycoprotein, resulting in attenuation of the virus in swine [Ref7336:Fernandez-Sainz et al., 2009]. 		19203774
4960	E2	Classical swine fever virus	920146		12657942	PestiV2gp1	AF326963	NP_075354	polyprotein		Virulence factor	MUTATION: A E2 mutant in Classical swine fever virus is attenuated in pigs [Ref7377:Maurer et al., 2005]. 		15837238
5088	P7				27885149				nonstructural protein p7		Virulence factor	Using reverse genetics, partial in-frame deletions of p7 were deleterious for virus growth, demonstrating that CSFV p7 function is critical for virus production in cell cultures. A panel of recombinant mutant CSFVs was created using alanine scanning mutagenesis of the p7 gene harboring sequential three- to six-amino-acid residue substitutions spanning the entire protein. These recombinant viruses allowed the identification of the regions within p7 that are critical for virus production in vitro. In vivo, some of these viruses were partially or completely attenuated in swine relative to the highly virulent parental CSFV Brescia strain, indicating a significant role of p7 in CSFV virulence [Ref7527:Gladue et al., 2012].		22496228
5096	N-Pro				27885143				N-Pro		Virulence factor	Here, for the moderately virulent CSFV strain Alfort/187 as well as for the highly virulent strain Eystrup we show that the respective N(pro)-deleted viruses are attenuated. Vaccination of pigs with either of the two deletion mutants resulted in the induction of a strong antibody response. Animals were protected against challenge with a lethal dose of highly virulent CSFV indicating that N(pro) deletion mutants are excellent candidates for a modified live virus vaccine.  [Ref7496:Mayer et al., 2004]; 		14670312
5113	NS4B				27885153				nonstructural protein NS4B		Virulence factor	NS4B.VGIv showed an attenuated phenotype in swine, displaying reduced replication in the oronasal cavity and limited spread from the inoculation site to secondary target organs [Ref7336:Fernandez-Sainz et al., 2009].		19203774