Protegen ID	Gene Name	Sequence Strain (Species/Organism)	NCBI Gene ID	NCBI Nucleotide GI	NCBI Protein GI	Locus Tag	Genbank Accession	Protein Accession	Protein Name	Protein Annotation	Molecule Role	Molecule Role Annotation	COG	PMID
5120	ORF2	Porcine circovirus 2	2943185		38018062	PCV2_gp3	AY424401	NP_937957	capsid protein		Virulence factor	After 120 passages, only two nucleotide mutations were identified in the entire genome, and both were located in the capsid gene: the mutations were located at nucleotide positions 328 (C328G) and 573 (A573C). The PCV2 genomic copy numbers in serum in the PCV2 VP1 group were significantly higher than those in the PCV2 VP120 group (P < 0.0001). Gross and histopathologic lesions in pigs inoculated with PCV2 VP1 were more severe than those inoculated with PCV2 VP120 at both day 21 and 42 necropsies (P = 0.0032 and P = 0.0274, respectively). Taken together, the results from this study indicated that the P110A and R191S mutations in the capsid of PCV2 enhanced the growth ability of PCV2 in vitro and attenuated the virus in vivo. This finding has important implications for PCV2 vaccine development.  [Ref7509:Fenaux et al., 2004]		15564454
5121	ORF1	Porcine circovirus 2	2943186		38018061	PCV2_gp1	AY424401	NP_937956	replicase		Virulence factor	The chimeric PCV1-2, containing the PCV2 capsid, PCV1 rep, and Ori genes, is attenuated in pigs.  Meanwhile, histological lesions of lymphoid and lung tissues, viral loads in lymphoid tissues, viremia, and TNF-α expression in PBMCs were not significantly different between groups PCV2b-Ori1 and PCV2b, suggesting that the Rep gene (ORF1) likely contributes to viral pathogenicity in vivo.  [Ref7511:Hua et al., 2013]		23454919