Protegen ID	Gene Name	Sequence Strain (Species/Organism)	NCBI Gene ID	NCBI Nucleotide GI	NCBI Protein GI	Locus Tag	Genbank Accession	Protein Accession	Protein Name	Protein Annotation	Molecule Role	Molecule Role Annotation	COG	PMID
4832	NS1	Influenza A virus (A/Puerto Rico/8/34(H1N1))	956533		8486133	FLUAVs8gp2	J02150	NP_040984	nonstructural protein NS1		Virulence factor	MUTATION: Influenza virus with a deletion of the NS1 gene were highly attenuated in mice [Ref7259:García-Sastre et al., 1998].; 		9878611
4833	PB1	Influenza A virus (A/Puerto Rico/8/34(H1N1))	956534		8486135	FLUAVs2gp1	J02151	NP_040985	polymerase 1		Virulence factor	MUTATION: mutations eliminating expression of PB1F2 without afffecting the PB1 open reading frame were attenuating following intranasal inoculation [Ref7260:Zamarin et al., 2006].; 		16873254
4984	M2	Influenza A virus (A/Puerto Rico/8/1934(H1N1))	956528		56583270	FLUAVs7gp2	V01099	NP_040979	matrix protein 2		Virulence factor	MUTATION: Mutant influenza virus that lacks the transmembrane and cytoplasmic tail domains of M2 (M2 knockout [M2KO]) from Influenza A virus (A/Puerto Rico/8/34(H1N1)) is attenuated in both cell culture and mice [Ref7399:Watanabe et al., 2009]. 		19321619
5090	HA			159144920	159144921			ABW90135.1	hemagglutinin		Virulence factor	Determination of virulence for mice indicated that amino acid substitution in the HA gene of a low-virulent escape mutant produced a decrease of virulence whereas the additional mutation identical to that acquired by the escape mutant in the course of readaptation restored the virulence to initial level. The findings are the first strong evidence for lower H5N1 virus virulence resulting from the amino acid substitution changing the antigenic specificity of HA and for restored virulence arising from compensating mutation in the HA gene.[Ref7490:Krylov et al., 2010]		21260989
5116	PB2	Influenza A virus (A/goose/Guangdong/1/1996(H5N1))	3654615		73852947	FLUAVH5N1_s1gp1	AF144300	YP_308664	polymerase PB2		Virulence factor	The recombinant viruses with the PB2 (63T) mutation were less pathogenic to mice.  [Ref7506:Li et al., 2011]		21367983
5117	PA	Influenza A virus (A/goose/Guangdong/1/1996(H5N1))	3654617		73852951	FLUAVH5N1_s3gp1	AF144302	YP_308666	polymerase PA		Virulence factor	The virulence of the reassortant bearing the PA gene from DK/49 in the GS/65 background increased 10(5)-fold relative to that of the GS/65 virus.   [Ref7507:Song et al., 2011]		21177821