Protegen ID Gene Name Sequence Strain (Species/Organism) NCBI Gene ID NCBI Nucleotide GI NCBI Protein GI Locus Tag Genbank Accession Protein Accession Protein Name Protein Annotation Molecule Role Molecule Role Annotation COG PMID 2 traT from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3244810 58000389 O2R_18 YP_190117.1 plays a role in APEC complement resistance; similar to protein family HMM PF05818 Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 3 traT from Escherichia coli EH41 Escherichia coli EH41 3654467 73853290 LH0131 YP_308786.1 Complement resistance protein Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 4 ehxA Escherichia coli 3654480 73853298 LH0144 AY258503 YP_308794 hemolysin A Virulence factor TOXIN: Ehx demonstrated lysis of BL-3 cells (cells from a bovine lymphoma cell line), sheep and human erythrocytes [Ref6229:Bauer and Welch, 1996]. 8557336 5 ehxC Escherichia coli 3654481 73853299 LH0145 AY258503 YP_308795 hemolysin C Virulence factor TOXIN: Ehx demonstrated lysis of BL-3 cells (cells from a bovine lymphoma cell line), sheep and human erythrocytes [Ref6229:Bauer and Welch, 1996]. 8557336 6 eatA from Escherichia coli (strain: JEF100) Escherichia coli (strain: JEF100) 3829299 83404842 pCoo056 YP_424856.1 secreted autotransporter serine protease EatA Virulence factor MUTATION: interruption of the eatA gene retarded fluid accumulation in the rabbit ileal loop model, suggesting that this autotransporter contributes to the virulence of ETEC [Ref6230:Patel et al., 2004]. 14977988 7 traT from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853397 84060756 O2ColV112 YP_443958.1 surface exclusion protein; similar to GenBank Accession Number AAW58918 Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 8 iroB from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853505 84060896 O2ColV53 YP_444106.1 iron-related glycotransferase; similar to NP_753168 Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. 18541653 9 iroC from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853506 84060897 O2ColV54 YP_444107.1 iron-related ABC transporter protein; similar to GenBank Accession Number AAN76099 Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. 18541653 10 iroD from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853507 84060898 O2ColV55 YP_444108.1 iron-related ferric enterochelin esterase; similar to GenBank Accession Number AAN76100 Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. 18541653 11 iroN from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853509 84060900 O2ColV57 YP_444110.1 iron-related siderophore receptor; similar to GenBank Accession Number AAN76093 Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum [Ref6232:Fischbach et al., 2006]. 17060628 12 iucA from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853516 84060856 O2ColV11 YP_444058.1 aerobactin biosynthesis protein; similar to GenBank Accession Number CAA53707 Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. 2973985 13 iucB from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853517 84060857 O2ColV12 YP_444059.1 N-hydroxylysine acetylase; aerobactin biosynthesis protein; similar to GenBank Accession Number CAH64815 Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. 2973985 14 iucC from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853518 84060858 O2ColV13 YP_444060.1 aerobactin biosynthesis protein; similar to GenBank Accession Number CAH64814 Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. 2973985 15 iucD from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853519 84060859 O2ColV14 YP_444061.1 L-lysine 6-monooxigenase; similar to GenBank Accession Number CAE55773 Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. 2973985 16 iutA from Escherichia coli (strain: A2363) Escherichia coli (strain: A2363) 3853520 84060860 O2ColV15 YP_444062.1 ferric aerobactin receptor; similar to GenBank Accession Number CAE55774 Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. 11553558 17 traT from Escherichia coli (strain: DIJ1, serotype: O86:H-) Escherichia coli (strain: DIJ1, serotype: O86:H-) 4364265 116006905 pO86A1_p122 YP_788088.1 TraT complement resistance protein precursor Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 18 bfpA from Escherichia coli (strain: E2348/69, serotype: O127:H6) Escherichia coli (strain: E2348/69, serotype: O127:H6) 6382168 190014867 MAR003 YP_001965379.1 Major pilin structural unit bundlin Virulence factor MUTATION: bfpA deletion mutant of EPEC was less virulent in humans than the wild-type EPEC [Ref6235:Bieber et al., 1998]. 9641917 19 bfpB from Escherichia coli (strain: E2348/69, serotype: O127:H6) Escherichia coli (strain: E2348/69, serotype: O127:H6) 6382170 190014869 MAR005 YP_001965381.1 Lipoprotein Virulence factor MUTATION: bfpB mutant EPEC is unable to form BFP which is a major virulence factor of EPEC [Ref6236:Ramer et al., 1996]. 8932312 20 bfpE from Escherichia coli (strain: E2348/69, serotype: O127:H6) Escherichia coli (strain: E2348/69, serotype: O127:H6) 6382174 190014873 MAR009 YP_001965385.1 Transmembrane protein Virulence factor MUTATION: bfpE mutant strain of EPEC fails to form detectable BFP and fails to carry out autoaggregation and localized adherence to HEp-2 epithelial cells in tissue culture [Ref6237:Blank and Donnenberg, 2001]. 11443077 21 bfpF from Escherichia coli (strain: E2348/69, serotype: O127:H6) Escherichia coli (strain: E2348/69, serotype: O127:H6) 6382175 190014874 MAR010 YP_001965386.1 Nucleotide binding protein Virulence factor MUTATION: bfpF mutant colonized the human intestine but was about 200-fold less virulent than the wild-type EPEC [Ref6235:Bieber et al., 1998]. 9641917 22 bfpP from Escherichia coli (strain: E2348/69, serotype: O127:H6) Escherichia coli (strain: E2348/69, serotype: O127:H6) 6382176 190014875 MAR011 YP_001965387.1 Prepilin peptidase Virulence factor MUTATION: mutation in bfpP blocks BFP biogenesis and localized adherence to host cells by EPEC [Ref6238:Anantha et al., 2000]. 10762251 23 perA Escherichia coli 6382186 190014885 MAR020 YP_001965397.1 Transcriptional activator, bfp operon Virulence factor MUTATION: bfpT (perA) disruption mutant of EPEC was less virulent in humans than the wild-type EPEC [Ref6235:Bieber et al., 1998]. 9641917 2026 bfpA from Escherichia coli (strain: B171) Escherichia coli (strain: B171) 1238603 10955353 pB171_003 NP_053065.1 Bundlin Virulence factor MUTATION: bfpA deletion mutant of EPEC was less virulent in humans than the wild-type EPEC [Ref6235:Bieber et al., 1998]. 9641917 2027 bfpB from Escherichia coli (strain: B171) Escherichia coli (strain: B171) 1238604 10955355 pB171_005 NP_053067.1 BfpB Virulence factor MUTATION: bfpB mutant EPEC is unable to form BFP which is a major virulence factor of EPEC [Ref6236:Ramer et al., 1996]. 8932312 2028 bfpE from Escherichia coli (strain: B171) Escherichia coli (strain: B171) 1238607 10955359 pB171_009 NP_053071.1 BfpE Virulence factor MUTATION: bfpE mutant strain of EPEC fails to form detectable BFP and fails to carry out autoaggregation and localized adherence to HEp-2 epithelial cells in tissue culture [Ref6237:Blank and Donnenberg, 2001]. 11443077 2029 bfpF from Escherichia coli (strain: B171) Escherichia coli (strain: B171) 1238608 10955360 pB171_010 NP_053072.1 BfpF Virulence factor MUTATION: bfpF mutant colonized the human intestine but was about 200-fold less virulent than the wild-type EPEC [Ref6235:Bieber et al., 1998]. 9641917 2030 bfpP from Escherichia coli (strain: B171) Escherichia coli (strain: B171) 1238616 10955361 pB171_011 NP_053073.1 prepilin peptidase Virulence factor MUTATION: mutation in bfpP blocks BFP biogenesis and localized adherence to host cells by EPEC [Ref6238:Anantha et al., 2000]. 10762251 2031 bfpT Escherichia coli 1238617 10955374 pB171_024 EF011039 NP_053086 BfpT Virulence factor MUTATION: bfpT (perA) disruption mutant of EPEC was less virulent in humans than the wild-type EPEC [Ref6235:Bieber et al., 1998]. 9641917 2032 rfaH from Escherichia coli E24377A Escherichia coli E24377A 5586647 157157494 EcE24377A_4360 YP_001465326.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 2033 dppA from Escherichia coli E24377A Escherichia coli E24377A 5586739 157159059 EcE24377A_4037 YP_001465018.1 dipeptide ABC transporter, periplasmic dipeptide-binding protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 2034 fliC from Escherichia coli E24377A Escherichia coli E24377A 5586916 157159035 EcE24377A_2158 YP_001463228.1 flagellin FliC Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 2035 ompA from Escherichia coli E24377A Escherichia coli E24377A 5589214 157159269 EcE24377A_1071 YP_001462189.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 2036 fimH from Escherichia coli E24377A Escherichia coli E24377A 5589412 157157381 EcE24377A_4917 YP_001465845.1 protein FimH Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 2037 dsbA from Escherichia coli E24377A Escherichia coli E24377A 5590815 157155214 EcE24377A_4378 YP_001465340.1 thiol:disulfide interchange protein DsbA Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 2038 dsbA from Escherichia coli HS Escherichia coli HS 5591486 157163329 EcHS_A4083 YP_001460647.1 thiol:disulfide interchange protein DsbA Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 2039 fimH from Escherichia coli HS Escherichia coli HS 5591791 157163764 EcHS_A4546 YP_001461082.1 protein FimH Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 2040 ompA from Escherichia coli HS Escherichia coli HS 5591810 157160478 EcHS_A1066 YP_001457796.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 2041 tonB from Escherichia coli HS Escherichia coli HS 5593249 157160757 EcHS_A1361 YP_001458075.1 outer membrane receptor-mediated transport energizer protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 2042 dppA from Escherichia coli HS Escherichia coli HS 5593889 157163019 EcHS_A3744 YP_001460337.1 dipeptide ABC transporter, periplasmic dipeptide-binding protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 2043 rfaH from Escherichia coli HS Escherichia coli HS 5594372 157163316 EcHS_A4064 YP_001460634.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 2044 mppA Escherichia coli APEC O1 4491551 117623583 APECO1_483 CP000468 YP_852496 periplasmic murein peptide-binding protein precursor Virulence factor MUTATION: mppA mutant is attenuated in chickens [Ref6646:Li et al., 2005]. COG4166E, under E: Amino acid transport and metabolism 15845486 2045 sbmA Escherichia coli APEC O1 4491947 117622640 APECO1_1631 CP000468 YP_851553 transport protein Virulence factor MUTATION: sbmA mutant is attenuated in chickens [Ref6646:Li et al., 2005]. COG1133I, under I: Lipid transport and metabolism 15845486 2046 fimH from Escherichia coli APEC O1 Escherichia coli APEC O1 4492146 117626628 APECO1_2110 YP_859951.1 type 1 fimbiral adhesin FimH Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 2047 ychF Escherichia coli 4492443 117623421 APECO1_319 YP_852334.1 translation-associated GTPase Virulence factor MUTATION: ychF mutant is attenuated in chickens [Ref6646:Li et al., 2005]. COG0012J, under J: Translation, ribosomal structure and biogenesis 15845486 2048 waaL Escherichia coli APEC O1 4492586 117625899 APECO1_2834 CP000468 YP_859222 lipid A-core, surface polymer ligase Virulence factor MUTATION: waaL mutant is attenuated in chickens [Ref6646:Li et al., 2005]. COG3307M, under M: Cell wall/membrane/envelope biogenesis 15845486 2049 dppA from Escherichia coli APEC O1 Escherichia coli APEC O1 4492758 117625828 APECO1_2904 YP_859151.1 dipeptide transporter protein DppA Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 2050 dsbA from Escherichia coli APEC O1 Escherichia coli APEC O1 4492849 117626134 APECO1_2601 YP_859457.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 2051 ibeA from Escherichia coli APEC O1 Escherichia coli APEC O1 4493341 117626645 APECO1_2093 YP_859968.1 invasion protein IbeA Virulence factor MUTATION: deletion mutant was significantly less invasive in human brain microvascular endothelial cells (BMECs) than the parent strain (E. coli K1) [Ref6647:Huang et al., 2001]. 11237832 2052 papA from Escherichia coli APEC O1 Escherichia coli APEC O1 4493551 117625209 APECO1_3519 YP_854256.1 major fibrial protein Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence [Ref6648:Johnson, 1991]. COG3539NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 1672263 2053 sitB Escherichia coli 4493636 117623375 APECO1_273 YP_852288.1 ABC Mn+2/Fe+2 transporter, ATPase subunit SitB Virulence factor MUTATION: sitB mutant is attenuated in chickens [Ref6646:Li et al., 2005]. COG1121P, under P: Inorganic ion transport and metabolism 15845486 2054 malX Escherichia coli APEC O1 4493654 117623807 APECO1_704 CP000468 YP_852720 bifunctional PTS system maltose and glucose-specific transporter subunits IICB Virulence factor MUTATION: malX mutant is attenuated in chickens [Ref6646:Li et al., 2005]. COG1263G, under G: Carbohydrate transport and metabolism 15845486 2055 ompA from Escherichia coli APEC O1 Escherichia coli APEC O1 4493784 117623167 APECO1_62 YP_852080.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 2056 tia from Escherichia coli APEC O1 Escherichia coli APEC O1 4493822 117625198 APECO1_3530 YP_854245.1 Tia invasion determinant Virulence factor MUTATION: tia deletion strains were noninvasive and lacked the ability to adhere to human ileocecal cells [Ref6649:Fleckenstein et al., 1996]. COG3637M, under M: Cell wall/membrane/envelope biogenesis 8675335 2057 papG from Escherichia coli APEC O1 Escherichia coli APEC O1 4493827 117625201 APECO1_3527 YP_854248.1 allele II Virulence factor MUTATION: attenuated to human neutrophils in vitro [Ref6650:Tewari et al., 1994]. 7960108 2058 rfaH from Escherichia coli APEC O1 Escherichia coli APEC O1 4494179 117626120 APECO1_2615 YP_859443.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 2059 kpsS Escherichia coli APEC O1 4494206 117625251 APECO1_3477 CP000468 YP_854344 polysialic acid capsule synthesis protein KpsS Virulence factor MUTATION: kpsS mutant is attenuated in chickens [Ref6646:Li et al., 2005]. COG3562M, under M: Cell wall/membrane/envelope biogenesis 15845486 2060 wcaE Escherichia coli APEC O1 4494493 117624251 APECO1_1145 CP000468 YP_853164 putative glycosyl transferase Virulence factor MUTATION: wcaE mutant is attenuated in chickens [Ref6646:Li et al., 2005]. COG0463M, under M: Cell wall/membrane/envelope biogenesis 15845486 2061 APECO1_3367 Escherichia coli APEC O1 4494532 117625360 APECO1_3367 CP000468 YP_855140 putative Yqi fimbrial adhesin Virulence factor MUTATION: yqi mutant is attenuated in chickens [Ref6651:Antão et al., 2009]. 19907658 2062 fliC from Escherichia coli APEC O1 Escherichia coli APEC O1 4494642 117624065 APECO1_964 YP_852978.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 2063 yjjQ Escherichia coli APEC O1 4494948 117626679 APECO1_2059 CP000468 YP_860002 hypothetical protein Virulence factor MUTATION: yjjQ mutant is attenuated in chickens [Ref6652:Li et al., 2008]. COG2197TK, under T: Signal transduction mechanisms; K: Transcription 18375801 2064 chuA from Escherichia coli APEC O1 Escherichia coli APEC O1 4495129 117625783 APECO1_2948 YP_859106.1 outer membrane heme/hemoglobin receptor ChuA Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme [Ref6653:Torres and Payne, 1997]. COG4771P, under P: Inorganic ion transport and metabolism 9157252 2065 tktA Escherichia coli APEC O1 4495170 117625164 APECO1_3594 CP000468 YP_854152 transketolase Virulence factor MUTATION: tktA mutant is attenuated in chickens [Ref6646:Li et al., 2005]. COG0021G, under G: Carbohydrate transport and metabolism 15845486 2066 tonB from Escherichia coli APEC O1 Escherichia coli APEC O1 4495352 117623468 APECO1_368 YP_852381.1 membrane spanning protein in TonB-ExbB-ExbD complex; transduces proton motive force of the cytoplasmic membrane to outer membrane transporters; involved in the transport of ron-siderophore complexes, vitamin B12 and colicins Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 2067 rfaH from Escherichia coli 536 Escherichia coli 536 4187921 110644186 ECP_4055 YP_671916.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 2068 ECP_4655 Escherichia coli 536 4190232 110644762 ECP_4655 CP000247 YP_672492 FimH protein precursor Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 2069 dppA from Escherichia coli UTI89 Escherichia coli UTI89 3989640 91213056 UTI89_C4082 YP_543042.1 periplasmic dipeptide transport protein precursor Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 2070 cnf1 Escherichia coli UTI89 3990770 91213869 UTI89_C4921 CP000243 YP_543855 cytotoxic necrotizing factor 1 Virulence factor MUTATION: attenuated in mice [Ref6654:Rippere-Lampe et al., 2001]. 11349064 2071 hlyA from Escherichia coli UTI89 Escherichia coli UTI89 3990775 91213874 UTI89_C4926 YP_543860.1 hemolysin A Virulence factor MUTATION: attenuated in zebra fish [Ref6655:Wiles et al., 2009]. COG2931Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 20019794 2072 hlyC from Escherichia coli UTI89 Escherichia coli UTI89 3990776 91213875 UTI89_C4927 YP_543861.1 hemolysin C Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells [Ref6656:Stanley et al., 1996]. COG2994O, under O: Posttranslational modification, protein turnover, chaperones 8793877 2073 chuA from Escherichia coli UTI89 Escherichia coli UTI89 3991259 91213002 UTI89_C4028 YP_542988.1 outer membrane hemin receptor ChuA Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme [Ref6653:Torres and Payne, 1997]. COG4771P, under P: Inorganic ion transport and metabolism 9157252 2074 iroN from Escherichia coli UTI89 Escherichia coli UTI89 3991364 91210147 UTI89_C1118 YP_540133.1 IroN protein Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum [Ref6232:Fischbach et al., 2006]. COG4771P, under P: Inorganic ion transport and metabolism 17060628 2075 iroD from Escherichia coli UTI89 Escherichia coli UTI89 3991366 91210149 UTI89_C1120 YP_540135.1 IroD protein Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. COG2382P, under P: Inorganic ion transport and metabolism 18541653 2076 iroC from Escherichia coli UTI89 Escherichia coli UTI89 3991367 91210150 UTI89_C1121 YP_540136.1 IroC protein Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. COG1132V, under V: Defense mechanisms 18541653 2077 iroB from Escherichia coli UTI89 Escherichia coli UTI89 3991368 91210151 UTI89_C1122 YP_540137.1 putative glucosyltransferase Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. COG1819GC, under G: Carbohydrate transport and metabolism; C: Energy production and conversion 18541653 2078 tonB from Escherichia coli UTI89 Escherichia coli UTI89 3991369 91210476 UTI89_C1451 YP_540462.1 membrane spanning protein in TonB-ExbB-ExbD complex; transduces proton motive force of the cytoplasmic membrane to outer membrane transporters; involved in the transport of ron-siderophore complexes, vitamin B12 and colicins Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 2079 papG from Escherichia coli UTI89 Escherichia coli UTI89 3992036 91213835 UTI89_C4887 YP_543821.1 p pilus adhesin PapG protein Virulence factor MUTATION: attenuated to human neutrophils in vitro [Ref6650:Tewari et al., 1994]. 7960108 2080 papA from Escherichia coli UTI89 Escherichia coli UTI89 3992044 91213843 UTI89_C4895 YP_543829.1 major pilin subunit PapA Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence [Ref6648:Johnson, 1991]. COG3539NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 1672263 2081 fimH from Escherichia coli UTI89 Escherichia coli UTI89 3992235 91213965 UTI89_C5017 YP_543951.1 type 1 fimbiral adhesin FimH Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 2082 ibeA from Escherichia coli UTI89 Escherichia coli UTI89 3992253 91213983 UTI89_C5035 YP_543969.1 invasion protein IbeA Virulence factor MUTATION: deletion mutant was significantly less invasive in human brain microvascular endothelial cells (BMECs) than the parent strain (E. coli K1) [Ref6647:Huang et al., 2001]. 11237832 2083 aslA from Escherichia coli UTI89 Escherichia coli UTI89 3992515 91213321 UTI89_C4359 YP_543307.1 arylsulfatase Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 [Ref6657:Hoffman et al., 2000]. COG3119P, under P: Inorganic ion transport and metabolism 10948126 2084 dsbA from Escherichia coli UTI89 Escherichia coli UTI89 3992605 91213404 UTI89_C4447 YP_543390.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 2085 fliC from Escherichia coli UTI89 Escherichia coli UTI89 3993212 91211142 UTI89_C2124 YP_541128.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 2086 rfaH from Escherichia coli UTI89 Escherichia coli UTI89 3993833 91213389 UTI89_C4427 YP_543375.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 2087 ompA from Escherichia coli UTI89 Escherichia coli UTI89 3994721 91210052 UTI89_C1022 YP_540038.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 2088 iroN from Escherichia coli CFT073 Escherichia coli CFT073 1034931 26247124 c1250 NP_753164.1 Siderophore receptor IroN Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum [Ref6232:Fischbach et al., 2006]. COG4771P, under P: Inorganic ion transport and metabolism 17060628 2089 iroD from Escherichia coli CFT073 Escherichia coli CFT073 1034933 26247126 c1252 NP_753166.1 Ferric enterochelin esterase Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. COG2382P, under P: Inorganic ion transport and metabolism 18541653 2090 iroC from Escherichia coli CFT073 Escherichia coli CFT073 1034934 26247127 c1253 NP_753167.1 ATP binding cassette (ABC) transporter homolog Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. COG1132V, under V: Defense mechanisms 18541653 2091 iroB from Escherichia coli CFT073 Escherichia coli CFT073 1034935 26247128 c1254 NP_753168.1 Putative glucosyltransferase Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. COG1819GC, under G: Carbohydrate transport and metabolism; C: Energy production and conversion 18541653 2092 tonB from Escherichia coli CFT073 Escherichia coli CFT073 1035499 26247582 c1717 NP_753622.1 membrane spanning protein in TonB-ExbB-ExbD complex; transduces proton motive force of the cytoplasmic membrane to outer membrane transporters; involved in the transport of ron-siderophore complexes, vitamin B12 and colicins Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 2093 ycjM Escherichia coli CFT073 1035566 26247644 c1780 AE014075 NP_753684 putative sucrose phosphorylase Virulence factor MUTATION: ycjM mutant is attenuated in chickens [Ref6646:Li et al., 2005]. COG0366G, under G: Carbohydrate transport and metabolism 15845486 2094 rfaH from Escherichia coli CFT073 Escherichia coli CFT073 1035846 26250608 c4789 NP_756648.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 2095 wecE Escherichia coli CFT073 1036493 26250530 c4711 AE014075 NP_756570 TDP-4-oxo-6-deoxy-D-glucose transaminase Virulence factor MUTATION: wecE mutant is attenuated in mice [Ref6658:Bahrani-Mougeot et al., 2002]. COG0399M, under M: Cell wall/membrane/envelope biogenesis 12180926 2096 dsbA from Escherichia coli CFT073 Escherichia coli CFT073 1036525 26250621 c4804 NP_756661.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 2097 focA Escherichia coli CFT073 1036891 26247113 c1239 AE014075 NP_753153 F1C major fimbrial subunit precursor Virulence factor MUTATION: focA deletion mutants were unable to bind to brain glycolipids [Ref6659:Prasadarao et al., 1993]. COG3539NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 8098039 2098 fliC from Escherichia coli CFT073 Escherichia coli CFT073 1036977 26248190 c2338 NP_754230.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 2099 chuA from Escherichia coli CFT073 Escherichia coli CFT073 1037196 26250130 c4308 NP_756170.1 Outer membrane heme/hemoglobin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme [Ref6653:Torres and Payne, 1997]. COG4771P, under P: Inorganic ion transport and metabolism 9157252 2100 fimH from Escherichia coli CFT073 Escherichia coli CFT073 1037233 26251208 c5400 NP_757248.1 FimH protein precursor Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 2101 fimF Escherichia coli CFT073 1037237 26251205 c5397 AE014075 NP_757245 FimF protein precursor Virulence factor MUTATION: fimF mutant is attenuated in mice [Ref6658:Bahrani-Mougeot et al., 2002]. COG3539NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 12180926 2102 fimD Escherichia coli CFT073 1037242 26251204 c5396 AE014075 NP_757244 Outer membrane usher protein fimD precursor Virulence factor MUTATION: fimD mutant is attenuated in mice [Ref6658:Bahrani-Mougeot et al., 2002]. COG3188NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 12180926 2103 fimC Escherichia coli CFT073 1037243 26251203 c5395 AE014075 NP_757243 chaperone protein fimC precursor Virulence factor MUTATION: fimC mutant is attenuated in mice [Ref6658:Bahrani-Mougeot et al., 2002]. COG3121NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 12180926 2104 fimI from E. coli CFT073 Escherichia coli CFT073 1037245 26251202 c5394 NP_757242.1 Fimbrin-like protein fimI precursor Virulence factor MUTATION: fimI mutant is attenuated in mice [Ref6658:Bahrani-Mougeot et al., 2002]. COG3539NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 12180926 2105 fimB Escherichia coli CFT073 1037250 26251199 c5391 AE014075 NP_757239 tyrosine recombinase Virulence factor MUTATION: fimB mutant is attenuated in mice [Ref6658:Bahrani-Mougeot et al., 2002]. COG4974L, under L: Replication, recombination and repair 12180926 2106 dppA from Escherichia coli CFT073 Escherichia coli CFT073 1037335 26250183 c4361 NP_756223.1 Periplasmic dipeptide transport protein precursor Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 2107 manB from Escherichia coli CFT073 Escherichia coli CFT073 1037835 26248405 c2557 NP_754445.1 Phosphomannomutase Virulence factor MUTATION: manB mutant is attenuated in mice [Ref6658:Bahrani-Mougeot et al., 2002]. COG1109G, under G: Carbohydrate transport and metabolism 12180926 2108 waaW Escherichia coli CFT073 1038245 26250272 c4450 AE014075 NP_756312 UDP-galactose:(galactosyl) LPS alpha1,2-galactosyltransferase Virulence factor MUTATION: waaW mutant is attenuated in chickens [Ref6646:Li et al., 2005]. COG1442M, under M: Cell wall/membrane/envelope biogenesis 15845486 2109 hlyC from Escherichia coli CFT073 Escherichia coli CFT073 1039495 26249404 c3569 NP_755444.1 Hemolysin C Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells [Ref6656:Stanley et al., 1996]. COG2994O, under O: Posttranslational modification, protein turnover, chaperones 8793877 2110 hlyA from Escherichia coli CFT073 Escherichia coli CFT073 1039496 26249405 c3570 NP_755445.1 Hemolysin A Virulence factor MUTATION: attenuated in zebra fish [Ref6655:Wiles et al., 2009]. COG2931Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 20019794 2111 papG from Escherichia coli CFT073 Escherichia coli CFT073 1039518 26249418 c3583 NP_755458.1 PapG protein Virulence factor MUTATION: attenuated to human neutrophils in vitro [Ref6650:Tewari et al., 1994]. 7960108 2112 papA from Escherichia coli CFT073 Escherichia coli CFT073 1039530 26249427 c3592 NP_755467.1 PapA protein Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence [Ref6648:Johnson, 1991]. COG3539NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 1672263 2113 sat from Escherichia coli CFT073 Escherichia coli CFT073 1039573 26249454 c3619 NP_755494.1 Aecreted auto transpoter toxin Virulence factor MUTATION: Sat elicits cytopathic activity that damages host tissue and may increase the ability of E. coli to propagate [Ref6660:Guyer et al., 2002]. COG4625S, under S: Function unknown 12117966 2114 iutA from Escherichia coli CFT073 Escherichia coli CFT073 1039578 26249458 c3623 NP_755498.1 IutA protein Virulence factor MUTATION: mutant attenuated in mice competition assay [Ref6234:Torres et al., 2001]. COG1629P, under P: Inorganic ion transport and metabolism 11553558 2115 iucD from Escherichia coli CFT073 Escherichia coli CFT073 1039579 26249459 c3624 NP_755499.1 IucD protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG3486Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 2116 iucC from Escherichia coli CFT073 Escherichia coli CFT073 1039580 26249460 c3625 NP_755500.1 IucC protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 2117 iucB from Escherichia coli CFT073 Escherichia coli CFT073 1039581 26249461 c3626 NP_755501.1 IucB protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG1670J, under J: Translation, ribosomal structure and biogenesis 2973985 2118 iucA from Escherichia coli CFT073 Escherichia coli CFT073 1039582 26249462 c3627 NP_755502.1 IucA protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 2119 kpsC Escherichia coli CFT073 1039730 26249525 c3690 AE014075 NP_755565 KpsC protein Virulence factor MUTATION: kpsC mutant is attenuated in mice [Ref6658:Bahrani-Mougeot et al., 2002]. COG3563M, under M: Cell wall/membrane/envelope biogenesis 12180926 2120 pyrD Escherichia coli CFT073 1039998 26246966 c1081 AE014075 NP_753006 dihydroorotate dehydrogenase 2 Virulence factor MUTATION: pyrD mutant is attenuated in mice [Ref6658:Bahrani-Mougeot et al., 2002]. COG0167F, under F: Nucleotide transport and metabolism 12180926 2121 ompA from Escherichia coli CFT073 Escherichia coli CFT073 1039999 26246978 c1093 NP_753018.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 2122 phoU Escherichia coli CFT073 1040241 161486057 c4648 AE014075 NP_756506 transcriptional regulator PhoU Virulence factor MUTATION: phoU mutant is attenuated in mice [Ref6658:Bahrani-Mougeot et al., 2002]. COG0704P, under P: Inorganic ion transport and metabolism 12180926 2123 rfaH from Escherichia coli O157:H7 str. Sakai Escherichia coli O157:H7 str. Sakai 915134 15834024 ECs4770 NP_312797.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 2124 stx1B Escherichia coli O157:H7 str. EDL933 956702 15802645 Z3343 AE005174 NP_288672 shiga-like toxin 1 subunit B encoded within prophage CP-933V Virulence factor TOXIN: Stx induces apoptosis in HeLa cells [Ref6661:Cherla et al., 2003]. 14638419 2125 map from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 956892 15799850 Z0178 NP_285862.1 methionine aminopeptidase Virulence factor MUTATION: map mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG0024J, under J: Translation, ribosomal structure and biogenesis 15528651 2126 Z3341 Escherichia coli O157:H7 str. EDL933 956966 15802643 Z3341 AE005174 NP_288670 hypothetical protein Virulence factor MUTATION: Z3341 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2127 stx1A Escherichia coli O157:H7 str. EDL933 956968 15802646 Z3344 AE005174 NP_288673 shiga-like toxin 1 subunit A encoded within prophage CP-933V Virulence factor TOXIN: Stx induces apoptosis in HeLa cells [Ref6661:Cherla et al., 2003]. 14638419 2128 Z0275 Escherichia coli O157:H7 str. EDL933 957027 15799923 Z0275 AE005174 NP_285935 hypothetical protein Virulence factor MUTATION: Z0275 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG5433L, under L: Replication, recombination and repair 15528651 2129 sanA Escherichia coli O157:H7 str. EDL933 957084 15802700 Z3399 AE005174 NP_288727 hypothetical protein Virulence factor MUTATION: sanA mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG2949S, under S: Function unknown 15528651 2130 mglA Escherichia coli O157:H7 str. EDL933 957113 15802705 Z3404 AE005174 NP_288732 galactose/methyl galaxtoside transporter ATP-binding protein Virulence factor MUTATION: mglA mutant is attenuated in calves [Ref6663:van et al., 2005]. COG1129G, under G: Carbohydrate transport and metabolism 15731074 2131 Z0390 Escherichia coli O157:H7 str. EDL933 957204 15800026 Z0390 AE005174 NP_286038 hypothetical protein Virulence factor MUTATION: Z0390 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2132 Z3496 Escherichia coli O157:H7 str. EDL933 957222 15802787 Z3496 AE005174 NP_288814 hypothetical protein Virulence factor MUTATION: Z3496 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2133 Z0469 Escherichia coli 957315 15800100 Z0469 NP_286112.1 putative structural protein (partial) Virulence factor MUTATION: Z0469 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG3468MU, under M: Cell wall/membrane/envelope biogenesis; U: Intracellular trafficking, secretion, and vesicular transport 15528651 2134 rdgC Escherichia coli O157:H7 str. EDL933 957342 15800120 Z0492 AE005174 NP_286132 recombination associated protein Virulence factor MUTATION: rdgC mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG2974L, under L: Replication, recombination and repair 15528651 2135 Z0609 Escherichia coli O157:H7 str. EDL933 957466 15800217 Z0609 AE005174 NP_286229 hypothetical protein Virulence factor MUTATION: Z0609 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2136 evgS Escherichia coli O157:H7 str. EDL933 957555 15802913 Z3632 AE005174 NP_288940 hybrid sensory histidine kinase in two-component regulatory system with EvgA Virulence factor MUTATION: evgS mutant is attenuated in calves [Ref6663:van et al., 2005]. COG0642T, under T: Signal transduction mechanisms 15731074 2137 yfhH Escherichia coli 957907 161367545 Z3841 NP_289117.2 putative DNA-binding transcriptional regulator Virulence factor MUTATION: yfhH mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG1737K, under K: Transcription 15528651 2138 trxC Escherichia coli O157:H7 str. EDL933 957981 15803110 Z3867 AE005174 NP_289141 thioredoxin 2 Virulence factor MUTATION: trxC mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG3118O, under O: Posttranslational modification, protein turnover, chaperones 15528651 2139 Z0990 Escherichia coli O157:H7 str. EDL933 957986 15800523 Z0990 AE005174 NP_286535 hypothetical protein Virulence factor MUTATION: Z0990 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2140 Z3950 Escherichia coli O157:H7 str. EDL933 958085 15803172 Z3950 AE005174 NP_289204 hypothetical protein Virulence factor MUTATION: Z3950 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2141 recD Escherichia coli O157:H7 str. EDL933 958288 15803338 Z4136 AE005174 NP_289371 exonuclease V subunit alpha Virulence factor MUTATION: recD mutant is attenuated in calves [Ref6663:van et al., 2005]. COG0507L, under L: Replication, recombination and repair 15731074 2142 kduI Escherichia coli O157:H7 str. EDL933 958313 15803363 Z4163 AE005174 NP_289396 5-keto-4-deoxyuronate isomerase Virulence factor MUTATION: kduI mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG3717G, under G: Carbohydrate transport and metabolism 15528651 2143 Z4200 Escherichia coli O157:H7 str. EDL933 958343 15803400 Z4200 AE005174 NP_289433 hypothetical protein Virulence factor MUTATION: z4200 (pkgA) mutant is attenuated in calves [Ref6663:van et al., 2005]. 15731074 2144 Z3635 Escherichia coli O157:H7 str. EDL933 958378 15802914 Z3635 AE005174 NP_288942 putative transporter YfdV Virulence factor MUTATION: z3635 (yfdV) mutant is attenuated in calves [Ref6663:van et al., 2005]. COG0679R, under R: General function prediction only 15731074 2145 Z4282 Escherichia coli O157:H7 str. EDL933 958414 15803476 Z4282 AE005174 NP_289509 hypothetical protein Virulence factor MUTATION: z4282 mutant is attenuated in calves [Ref6663:van et al., 2005]. 15731074 2146 speA Escherichia coli 958416 15803477 Z4283 NP_289510.1 arginine decarboxylase Virulence factor MUTATION: speA mutant is attenuated in calves [Ref6663:van et al., 2005]. COG1166E, under E: Amino acid transport and metabolism 15731074 2147 Z4318 Escherichia coli O157:H7 str. EDL933 958457 15803511 Z4318 AE005174 NP_289544 hypothetical protein Virulence factor MUTATION: Z4318 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG3972R, under R: General function prediction only 15528651 2148 pitB Escherichia coli O157:H7 str. EDL933 958466 15803532 Z4341 AE005174 NP_289565 partial putative transport protein Virulence factor MUTATION: pitB mutant is attenuated in calves [Ref6663:van et al., 2005]. COG0306P, under P: Inorganic ion transport and metabolism 15731074 2149 Z1126 Escherichia coli O157:H7 str. EDL933 958522 15800647 Z1126 AE005174 NP_286661 hypothetical protein Virulence factor MUTATION: Z1126 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2150 Z1129 Escherichia coli O157:H7 str. EDL933 958525 15800650 Z1129 AE005174 NP_286664 putative helicase Virulence factor MUTATION: z1129 mutant is attenuated in calves [Ref6663:van et al., 2005]. COG4951S, under S: Function unknown 15731074 2151 tdcE Escherichia coli O157:H7 str. EDL933 958549 15803653 Z4466 AE005174 NP_289686 formate acetyltransferase 3 Virulence factor MUTATION: tdcE mutant is attenuated in calves [Ref6663:van et al., 2005]. COG1882C, under C: Energy production and conversion 15731074 2152 Z1182 Escherichia coli O157:H7 str. EDL933 958755 15800703 Z1182 AE005174 NP_286717 hypothetical protein Virulence factor MUTATION: Z1182 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2153 Z1202 Escherichia coli O157:H7 str. EDL933 958789 15800723 Z1202 AE005174 NP_286737 hypothetical protein Virulence factor MUTATION: Z1202 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG1112L, under L: Replication, recombination and repair 15528651 2154 trxB Escherichia coli 958846 15800751 Z1232 NP_286765.1 thioredoxin reductase Virulence factor MUTATION: trxB mutant is attenuated in calves [Ref6663:van et al., 2005]. COG0492O, under O: Posttranslational modification, protein turnover, chaperones 15731074 2155 ompA from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 958948 15800816 Z1307 NP_286832.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 2156 Z1328 Escherichia coli O157:H7 str. EDL933 958973 15800834 Z1328 AE005174 NP_286850 hypothetical protein Virulence factor MUTATION: Z1328 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2157 yqeJ Escherichia coli O157:H7 str. EDL933 958999 15803368 Z4168 AE005174 NP_289401 hypothetical protein Virulence factor MUTATION: ygeJ mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2158 ymcC Escherichia coli O157:H7 str. EDL933 959050 15800907 Z1403 AE005174 NP_286923 putative regulator Virulence factor MUTATION: ymcC mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2159 stx2B from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 959107 15800961 Z1465 AE005174 NP_286977 shiga-like toxin II B subunit encoded by bacteriophage BP-933W Virulence factor TOXIN: Stx induces apoptosis in HeLa cells [Ref6661:Cherla et al., 2003]. 14638419 2160 pgaA Escherichia coli O157:H7 str. EDL933 959162 15801015 Z1526 AE005174 NP_287031 outer membrane protein PgaA Virulence factor MUTATION: ycdS mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2161 agaB Escherichia coli O157:H7 str. EDL933 959220 15803678 Z4492 AE005174 NP_289711 PTS system N-acetylgalactosamine-specific transporter subunit IIB Virulence factor MUTATION: agaB mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG3444G, under G: Carbohydrate transport and metabolism 15528651 2162 mdoC Escherichia coli O157:H7 str. EDL933 959379 15801164 Z1681 AE005174 NP_287181 glucans biosynthesis protein Virulence factor MUTATION: mdoC (ymdD) mutant is attenuated in calves [Ref6663:van et al., 2005]. 15731074 2163 ypjA Escherichia coli O157:H7 str. EDL933 959506 15803170 Z3948 AE005174 NP_289202 putative ATP-binding component of a transport system Virulence factor MUTATION: ypjA mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG3468MU, under M: Cell wall/membrane/envelope biogenesis; U: Intracellular trafficking, secretion, and vesicular transport 15528651 2164 serA Escherichia coli O157:H7 str. EDL933 959584 15803448 Z4251 AE005174 NP_289481 D-3-phosphoglycerate dehydrogenase Virulence factor MUTATION: serA mutant is attenuated in calves [Ref6663:van et al., 2005]. COG0111HE, under H: Coenzyme transport and metabolism; E: Amino acid transport and metabolism 15731074 2165 Z1829 Escherichia coli O157:H7 str. EDL933 959588 15801299 Z1829 AE005174 NP_287316 hypothetical protein Virulence factor MUTATION: z1829 mutant is attenuated in calves [Ref6663:van et al., 2005]. 15731074 2166 Z5946 Escherichia coli O157:H7 str. EDL933 959707 15804920 Z5946 AE005174 NP_290962 putative restriction modification enzyme S subunit Virulence factor MUTATION: Z5946 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG0732V, under V: Defense mechanisms 15528651 2167 fimH from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 959733 15804895 Z5918 NP_290936.1 minor fimbrial subunit, D-mannose specific adhesin Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 2168 fimE Escherichia coli O157:H7 str. EDL933 959740 15804888 Z5911 AE005174 NP_290929 tyrosine recombinase Virulence factor MUTATION: fimE mutant is attenuated in calves [Ref6663:van et al., 2005]. COG4974L, under L: Replication, recombination and repair 15731074 2169 yjhS Escherichia coli O157:H7 str. EDL933 959744 15804884 Z5905 AE005174 NP_290925 hypothetical protein Virulence factor MUTATION: yjhS mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2170 Z5886 Escherichia coli O157:H7 str. EDL933 959763 15804865 Z5886 AE005174 NP_290906 hypothetical protein Virulence factor MUTATION: Z5886 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2171 nrdD Escherichia coli O157:H7 str. EDL933 959802 15804828 Z5848 AE005174 NP_290869 anaerobic ribonucleoside triphosphate reductase Virulence factor MUTATION: nrdD mutant is attenuated in calves [Ref6663:van et al., 2005]. COG1328F, under F: Nucleotide transport and metabolism 15731074 2172 Z1930 Escherichia coli O157:H7 str. EDL933 959880 15801390 Z1930 AE005174 NP_287407 putative protease encoded within prophage CP-933X Virulence factor MUTATION: Z1930 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2173 yjeJ Escherichia coli O157:H7 str. EDL933 959978 15804737 Z5750 AE005174 NP_290778 hypothetical protein Virulence factor MUTATION: yjeJ mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2174 yjcF Escherichia coli O157:H7 str. EDL933 960058 15804658 Z5665 AE005174 NP_290699 hypothetical protein Virulence factor MUTATION: yjcF mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG1357S, under S: Function unknown 15528651 2175 arp Escherichia coli O157:H7 str. EDL933 960113 15804603 Z5608 AE005174 NP_290644 regulator of acetyl CoA synthetase Virulence factor MUTATION: arp mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2176 Z5444 Escherichia coli O157:H7 str. EDL933 960248 15804490 Z5444 AE005174 NP_290530 putative glycoporin Virulence factor MUTATION: Z5444 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2177 dsbA from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960300 15804445 Z5392 NP_290485.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 2178 rfaH from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960318 15804432 Z5362 NP_290472.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 2179 ilvG Escherichia coli O157:H7 str. EDL933 960401 15804359 Z5279 AE005174 NP_290399 acetolactate synthase 2 catalytic subunit Virulence factor MUTATION: ilvG mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG0028EH, under E: Amino acid transport and metabolism; H: Coenzyme transport and metabolism 15528651 2180 narG Escherichia coli O157:H7 str. EDL933 960477 15801455 Z2001 AE005174 NP_287472 nitrate reductase 1, alpha subunit Virulence factor MUTATION: narG mutant is attenuated in calves [Ref6663:van et al., 2005]. COG5013C, under C: Energy production and conversion 15731074 2181 tonB from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960504 15801478 Z2030 NP_287495.1 membrane spanning protein in TonB-ExbB-ExbD complex; transduces proton motive force of the cytoplasmic membrane to outer membrane transporters; involved in the transport of ron-siderophore complexes, vitamin B12 and colicins Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 2182 Z2056 Escherichia coli O157:H7 str. EDL933 960527 15801501 Z2056 AE005174 NP_287518 hypothetical protein Virulence factor MUTATION: z2056 mutant is attenuated in calves [Ref6663:van et al., 2005]. 15731074 2183 Z2142 Escherichia coli O157:H7 str. EDL933 960608 15801579 Z2142 AE005174 NP_287596 putative tail component of prophage CP-933O Virulence factor MUTATION: z2142 mutant is attenuated in calves [Ref6663:van et al., 2005]. COG4672S, under S: Function unknown 15731074 2184 Z2144 Escherichia coli O157:H7 str. EDL933 960610 15801581 Z2144 AE005174 NP_287598 putative tail component of prophage CP-933O Virulence factor MUTATION: Z2144 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG4723S, under S: Function unknown 15528651 2185 Z2149 Escherichia coli 960615 15801586 Z2149 NP_287603.1 hypothetical protein Virulence factor MUTATION: Z2149 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2186 Z2176 Escherichia coli O157:H7 str. EDL933 960638 15801609 Z2176 AE005174 NP_287626 hypothetical protein Virulence factor MUTATION: z2176 mutant is attenuated in calves [Ref6663:van et al., 2005]. 15731074 2187 ydeK Escherichia coli O157:H7 str. EDL933 960656 15801627 Z2195 AE005174 NP_287644 putative lipoprotein Virulence factor MUTATION: ydeK mutant is attenuated in calves [Ref6663:van et al., 2005]. 15731074 2188 Z2203 Escherichia coli O157:H7 str. EDL933 960662 15801633 Z2203 AE005174 NP_287650 putative fimbrial usher protein Virulence factor MUTATION: Z2203 mutation is attenuated in calves [Ref6662:Dziva et al., 2004]. COG3188NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 15528651 2189 Z2205 Escherichia coli O157:H7 str. EDL933 960664 15801635 Z2205 AE005174 NP_287652 putative fimbrial-like protein Virulence factor MUTATION: z2205 mutant is attenuated in calves [Ref6663:van et al., 2005]. COG3539NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 15731074 2190 Z2207 Escherichia coli O157:H7 str. EDL933 960666 15801637 Z2207 AE005174 NP_287654 putative oxidoreductase Virulence factor MUTATION: z2207 mutant is attenuated in calves [Ref6663:van et al., 2005]. COG0243C, under C: Energy production and conversion 15731074 2191 yddA Escherichia coli O157:H7 str. EDL933 960672 15801642 Z2212 AE005174 NP_287659 putative ATP-binding component of a transport system Virulence factor MUTATION: yddA mutant is attenuated in calves [Ref6663:van et al., 2005]. COG4178R, under R: General function prediction only 15731074 2192 Z5214 Escherichia coli O157:H7 str. EDL933 960719 15804316 Z5214 AE005174 NP_290355 hypothetical protein Virulence factor MUTATION: Z5214 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2193 Z5212 Escherichia coli O157:H7 str. EDL933 960725 15804314 Z5212 AE005174 NP_290353 hypothetical protein Virulence factor MUTATION: Z5212 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2194 Z5131 Escherichia coli O157:H7 str. EDL933 960841 15804240 Z5131 AE005174 NP_290279 hypothetical protein Virulence factor MUTATION: Z5131 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG0741M, under M: Cell wall/membrane/envelope biogenesis 15528651 2195 escC Escherichia coli O157:H7 str. EDL933 960846 15804236 Z5126 AE005174 NP_290275 hypothetical protein Virulence factor MUTATION: escC mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG1450NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 15528651 2196 Z5121 Escherichia coli O157:H7 str. EDL933 960851 15804231 Z5121 AE005174 NP_290270 hypothetical protein Virulence factor MUTATION: z5121(st22) mutant is attenuated in calves [Ref6663:van et al., 2005]. 15731074 2197 escV Escherichia coli O157:H7 str. EDL933 960852 15804230 Z5120 AE005174 NP_290269 hypothetical protein Virulence factor MUTATION: escV mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG4789U, under U: Intracellular trafficking, secretion, and vesicular transport 15528651 2198 escN from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960853 15804229 Z5119 NP_290268.1 escN Virulence factor MUTATION: deletion of escN abolished pathogenicity in infant rabbits compared to wild-type EHEC [Ref6664:Ritchie and Waldor, 2005]. COG1157NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 15731044 2199 sepQ Escherichia coli O157:H7 str. EDL933 960856 15804226 Z5116 AE005174 NP_290265 hypothetical protein Virulence factor MUTATION: sepQ mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG1886NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 15528651 2200 Z5114 Escherichia coli O157:H7 str. EDL933 960858 15804224 Z5114 AE005174 NP_290263 hypothetical protein Virulence factor MUTATION: Z5114 (cesF) mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2201 tir from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960860 15804222 Z5112 NP_290261.1 putative translocated intimin receptor protein Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture [Ref6665:Schüller et al., 2007]. 17474908 2202 eae from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960862 15804220 Z5110 NP_290259.1 intimin adherence protein Virulence factor MUTATION: attenuated in humans [Ref6666:Donnenberg et al., 1993]. 8376594 2203 espA from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960865 15804217 Z5107 NP_290256.1 secreted protein EspA Virulence factor MUTATION: deletion of espA in STEC abolished ability to attach to HeLa cells and induce actin rearrangements [Ref6667:Ebel et al., 1998]. 9786192 2204 espD from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960866 15804216 Z5106 NP_290255.1 secreted protein EspD Virulence factor MUTATION: espD deletion mutant of the enterohemorrhagic E. coli (EHEC) strain EDL933 had impaired attachment to HeLa cells [Ref6668:Kresse et al., 1999]. COG5613S, under S: Function unknown 10456938 2205 espB from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960867 15804215 Z5105 NP_290254.1 secreted protein EspB Virulence factor MUTATION: deletion mutant attenuated in humans compared to wild-type EPEC [Ref6669:Tacket et al., 2000]. 10816529 2206 espF from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 960871 15804211 Z5100 NP_290250.1 espF Virulence factor MUTATION: espF EPEC mutant is deficient in its ability to disrupt electrical resistance across a polarized monolayer of intestinal epithelial cells, suggesting that EspF is an effector molecule required for disrupting intestinal epithelial barrier function [Ref6670:McNamara et al., 2001]. 11238563 2207 yibD Escherichia coli 960931 15804159 Z5042 NP_290198.1 predicted glycosyl transferase Virulence factor MUTATION: yibD mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG0463M, under M: Cell wall/membrane/envelope biogenesis 15528651 2208 rhsA Escherichia coli O157:H7 str. EDL933 960959 15804134 Z5014 AE005174 NP_290173 rhsA protein in rhs element Virulence factor MUTATION: rhsA mutant is attenuated in calves [Ref6663:van et al., 2005]. COG3209M, under M: Cell wall/membrane/envelope biogenesis 15731074 2209 hrpA Escherichia coli O157:H7 str. EDL933 961000 15801734 Z2313 AE005174 NP_287752 ATP-dependent RNA helicase HrpA Virulence factor MUTATION: hrpA mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG1643L, under L: Replication, recombination and repair 15528651 2210 Z2318 Escherichia coli O157:H7 str. EDL933 961004 15801738 Z2318 AE005174 NP_287756 putative phosphatidate cytidiltransferase Virulence factor MUTATION: Z2318 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG4589R, under R: General function prediction only 15528651 2211 dppA from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 961074 15804087 Z4961 NP_290125.1 dipeptide transport protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 2212 dctA Escherichia coli O157:H7 str. EDL933 961092 15804072 Z4942 AE005174 NP_290108 C4-dicarboxylate transporter DctA Virulence factor MUTATION: dctA mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG1301C, under C: Energy production and conversion 15528651 2213 Z2368 Escherichia coli O157:H7 str. EDL933 961108 15801782 Z2368 AE005174 NP_287800 hypothetical protein Virulence factor MUTATION: Z2368 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2214 chuA from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 961176 15804044 Z4911 NP_290080.1 outer membrane heme/hemoglobin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme [Ref6653:Torres and Payne, 1997]. COG4771P, under P: Inorganic ion transport and metabolism 9157252 2215 yhiJ Escherichia coli 961212 15804024 Z4887 NP_290060.1 hypothetical protein Virulence factor MUTATION: yhiJ mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2216 Z3620 Escherichia coli O157:H7 str. EDL933 961230 15802901 Z3620 AE005174 NP_288928 hypothetical protein Virulence factor MUTATION: Z3620 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2217 ycjV Escherichia coli O157:H7 str. EDL933 961311 15801852 Z2463 AE005174 NP_287870 putative ATP-binding component of a transport system Virulence factor MUTATION: ycjV mutant is attenuated in calves [Ref6663:van et al., 2005]. COG3839G, under G: Carbohydrate transport and metabolism 15731074 2218 acnA Escherichia coli O157:H7 str. EDL933 961382 15801901 Z2532 AE005174 NP_287921 aconitate hydratase Virulence factor MUTATION: acnA mutant is attenuated in calves [Ref6663:van et al., 2005]. COG1048C, under C: Energy production and conversion 15731074 2219 trpB Escherichia coli O157:H7 str. EDL933 961399 15801917 Z2550 AE005174 NP_287938 tryptophan synthase subunit beta Virulence factor MUTATION: trpB mutant is attenuated in calves [Ref6663:van et al., 2005]. COG0133E, under E: Amino acid transport and metabolism 15731074 2220 Z6010 Escherichia coli O157:H7 str. EDL933 961413 15801929 Z6010 AE005174 NP_287950 hypothetical protein Virulence factor MUTATION: Z6010 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. 15528651 2221 trkA Escherichia coli O157:H7 str. EDL933 961474 15803817 Z4660 AE005174 NP_289851 potassium transporter peripheral membrane component Virulence factor MUTATION: trkA mutant is attenuated in calves [Ref6663:van et al., 2005]. COG0569P, under P: Inorganic ion transport and metabolism 15731074 2222 Z2702 Escherichia coli O157:H7 str. EDL933 961645 15802086 Z2702 AE005174 NP_288108 hypothetical protein Virulence factor MUTATION: Z2702 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG0437C, under C: Energy production and conversion 15528651 2223 Z2749 Escherichia coli O157:H7 str. EDL933 961691 15802132 Z2749 AE005174 NP_288154 hypothetical protein Virulence factor MUTATION: z2749 mutant is attenuated in calves [Ref6663:van et al., 2005]. 15731074 2224 Z2751 Escherichia coli O157:H7 str. EDL933 961692 15802133 Z2751 AE005174 NP_288155 hypothetical protein Virulence factor MUTATION: z2751 mutant is attenuated in calves [Ref6663:van et al., 2005]. COG3137M, under M: Cell wall/membrane/envelope biogenesis 15731074 2225 celF Escherichia coli O157:H7 str. EDL933 961704 15802145 Z2764 AE005174 NP_288167 6-phospho-beta-glucosidase Virulence factor MUTATION: celF mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG1486G, under G: Carbohydrate transport and metabolism 15528651 2226 cspC Escherichia coli O157:H7 str. EDL933 961796 15802236 Z2868 AE005174 NP_288259 cold shock-like protein CspC Virulence factor MUTATION: cspC mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG1278K, under K: Transcription 15528651 2227 Z2984 Escherichia coli O157:H7 str. EDL933 961896 15802336 Z2984 AE005174 NP_288362 putative serine acetlyltransferase of prophage CP-933T Virulence factor MUTATION: Z2984 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG1045E, under E: Amino acid transport and metabolism 15528651 2228 sdiA Escherichia coli O157:H7 str. EDL933 961914 15802351 Z3004 AE005174 NP_288377 DNA-binding transcriptional activator SdiA Virulence factor MUTATION: sidA mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG2771K, under K: Transcription 15528651 2229 fliC from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 961921 15802358 Z3013 NP_288384.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 2230 yedL Escherichia coli 961931 15802367 Z3022 NP_288393.1 hypothetical protein Virulence factor MUTAITON: yedL mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG1246E, under E: Amino acid transport and metabolism 15528651 2231 Z3023 Escherichia coli O157:H7 str. EDL933 961932 15802368 Z3023 AE005174 NP_288394 putative secreted protein Virulence factor MUTATION: Z3023 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG4886S, under S: Function unknown 15528651 2232 Z3026 Escherichia coli O157:H7 str. EDL933 961934 15802370 Z3026 GN097348 NP_288396 putative secreted protein Virulence factor MUTATION: Z3026 mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG4886S, under S: Function unknown 15528651 2233 Z3062 Escherichia coli O157:H7 str. EDL933 961974 15802403 Z3062 AE005174 NP_288429 hypothetical protein Virulence factor MUTATION: z3062 mutant is attenuated in calves [Ref6663:van et al., 2005]. COG2351R, under R: General function prediction only 15731074 2234 manB from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 962089 15802510 Z3194 NP_288536.1 phosphomannomutase Virulence factor MUTATION: manB mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG1109G, under G: Carbohydrate transport and metabolism 15528651 2235 manC Escherichia coli O157:H7 str. EDL933 962090 15802511 Z3195 AE005174 NP_288537 mannose-1-P guanosyltransferase Virulence factor MUTATION: manC mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG0836M, under M: Cell wall/membrane/envelope biogenesis 15528651 2236 fcI Escherichia coli O157:H7 str. EDL933 962092 15802513 Z3197 AE005174 NP_288539 fucose synthetase Virulence factor MUTATION: fcl mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG0451MG, under M: Cell wall/membrane/envelope biogenesis; G: Carbohydrate transport and metabolism 15528651 2237 wbdP Escherichia coli O157:H7 str. EDL933 962094 15802515 Z3199 AE005174 NP_288541 glycosyl transferase Virulence factor MUTATION: wbdP mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG0438M, under M: Cell wall/membrane/envelope biogenesis 15528651 2238 per Escherichia coli O157:H7 str. EDL933 962095 15802516 Z3200 AE005174 NP_288542 perosamine synthetase Virulence factor MUTATION: per mutant is attenuated in calves [Ref6662:Dziva et al., 2004]. COG0399M, under M: Cell wall/membrane/envelope biogenesis 15528651 2239 iutA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6143094 170680936 EcSMS35_3178 YP_001745168.1 ferric aerobactin receptor IutA Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG1629P, under P: Inorganic ion transport and metabolism 11553558 2240 rfaH from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6143458 170682425 EcSMS35_4222 YP_001746174.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 2241 iucB from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6144084 170681805 EcSMS35_3181 YP_001745171.1 aerobactin siderophore biosynthesis protein IucB Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG1670J, under J: Translation, ribosomal structure and biogenesis 2973985 2242 dsbA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6144184 170681207 EcSMS35_4241 YP_001746187.1 thiol:disulfide interchange protein DsbA Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 2243 dppA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6144283 170679624 EcSMS35_3863 YP_001745830.1 dipeptide ABC transporter, periplasmic dipeptide-binding protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 2244 fliC from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6144795 170682489 EcSMS35_1258 YP_001743319.1 flagellin FliC Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 2245 tonB from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6145010 170681605 EcSMS35_1880 YP_001743934.1 outer membrane receptor-mediated transport energizer protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 2246 iucD from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6145117 170682870 EcSMS35_3179 YP_001745169.1 L-lysine 6-monooxygenase IucD Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG3486Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 2247 fimH from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6145732 170681950 EcSMS35_4847 YP_001746758.1 protein FimH Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 2248 iucA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6145998 170683655 EcSMS35_3182 YP_001745172.1 aerobactin siderophore biosynthesis protein IucA Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 2249 aslA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6146022 170681296 EcSMS35_4166 YP_001746117.1 arylsulfatase Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 [Ref6657:Hoffman et al., 2000]. COG3119P, under P: Inorganic ion transport and metabolism 10948126 2250 ompA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6146246 170681860 EcSMS35_2163 YP_001744213.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 2251 ibeA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6146360 170682712 EcSMS35_4868 YP_001746779.1 invasion protein IbeA Virulence factor MUTATION: deletion mutant was significantly less invasive in human brain microvascular endothelial cells (BMECs) than the parent strain (E. coli K1) [Ref6647:Huang et al., 2001]. 11237832 2252 chuA from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6146648 170681293 EcSMS35_3802 YP_001745768.1 outer membrane heme/hemoglobin receptor ChuA Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme [Ref6653:Torres and Payne, 1997]. COG4771P, under P: Inorganic ion transport and metabolism 9157252 2253 iucC from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6147083 170679922 EcSMS35_3180 YP_001745170.1 aerobactin siderophore biosynthesis protein IucC Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 2254 traJ from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6106483 170650783 EcSMS35_A0041 YP_001739929.1 type IV secretion-like conjugative transfer system regulator TraJ Virulence factor MUTATION: traJ mutants of E. coli K1 are attenuated in rat pups and macrophages [Ref6671:Hill et al., 2004]. 14688129 2255 traT from Escherichia coli SMS-3-5 Escherichia coli SMS-3-5 6106527 170650825 EcSMS35_A0012 YP_001739900.1 type IV secretion-like conjugative transfer system complement resistance protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 2256 ompA from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 945571 16128924 b0957 NP_415477.1 outer membrane protein A (3a;II*;G;d) Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 2257 tonB from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 945843 16129213 b1252 NP_415768.1 membrane spanning protein in TonB-ExbB-ExbD complex Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 2258 cusC Escherichia coli str. K-12 substr. MG1655 946288 16128555 b0572 U00096 NP_415104 copper/silver efflux system, outer membrane component Virulence factor MUTATION: ibeB mutant is unable to invade BMEC and was attenuated in a newborn rat model [Ref6672:Huang et al., 1999]. COG1538MU, under M: Cell wall/membrane/envelope biogenesis; U: Intracellular trafficking, secretion, and vesicular transport 10225861 2259 dppA from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 948062 16131416 b3544 NP_418001.1 dipeptide transporter Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 2260 rfaL Escherichia coli str. K-12 substr. MG1655 948148 16131493 b3622 U00096 NP_418079 O-antigen ligase Virulence factor MUTATION: rfaL mutant is attenuated in rats [Ref6673:Gonzalez et al., 2001]. COG3307M, under M: Cell wall/membrane/envelope biogenesis 11430402 2261 setC Escherichia coli str. K-12 substr. MG1655 948177 16131529 b3659 U00096 NP_418115 predicted sugar efflux system Virulence factor MUTATION: yicK mutant is attenuated in calves [Ref6663:van et al., 2005]. COG2814G, under G: Carbohydrate transport and metabolism 15731074 2262 rfaH from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 948327 16131688 b3842 NP_418284.1 DNA-binding transcriptional antiterminator Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 2263 dsbA from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 948353 16131701 b3860 NP_418297.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 2264 yijP Escherichia coli str. K-12 substr. MG1655 948458 16131793 b3955 U00096 NP_418390 conserved inner membrane protein Virulence factor MUTATION: yijP mutant had reduced invasion of BMEC [Ref6674:Wang et al., 1999]. COG2194R, under R: General function prediction only 10456927 2265 fimH from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 948847 16132141 b4320 NP_418740.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in humans and mice [Ref6644:Wiles et al., 2008]. 18482721 2266 aslA from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 949015 16131653 b3801 NP_418245.1 acrylsulfatase-like enzyme Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 [Ref6657:Hoffman et al., 2000]. COG3119P, under P: Inorganic ion transport and metabolism 10948126 2267 fliC from Escherichia coli str. K-12 substr. MG1655 Escherichia coli str. K-12 substr. MG1655 949101 16129870 b1923 NP_416433.1 flagellar filament structural protein (flagellin) Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 2268 dsbA from Escherichia coli str. K-12 substr. DH10B Escherichia coli str. K-12 substr. DH10B 6058256 170083333 ECDH10B_4049 YP_001732653.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 2269 tonB from Escherichia coli str. K-12 substr. DH10B Escherichia coli str. K-12 substr. DH10B 6058282 170080929 ECDH10B_1367 YP_001730249.1 membrane spanning protein in TonB-ExbB-ExbD complex Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 2270 dppA from Escherichia coli str. K-12 substr. DH10B Escherichia coli str. K-12 substr. DH10B 6058698 170083053 ECDH10B_3723 YP_001732373.1 dipeptide transporter; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 2271 rfaH from Escherichia coli str. K-12 substr. DH10B Escherichia coli str. K-12 substr. DH10B 6058984 170083320 ECDH10B_4031 YP_001732640.1 DNA-binding transcriptional antiterminator Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 2272 ompA from Escherichia coli str. K-12 substr. DH10B Escherichia coli str. K-12 substr. DH10B 6060328 170080615 ECDH10B_1027 YP_001729935.1 outer membrane protein A (3a;II*;G;d) Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 2273 aslA from Escherichia coli str. K-12 substr. DH10B Escherichia coli str. K-12 substr. DH10B 6061059 170083285 ECDH10B_3991 YP_001732605.1 acrylsulfatase-like enzyme Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 [Ref6657:Hoffman et al., 2000]. COG3119P, under P: Inorganic ion transport and metabolism 10948126 2274 toxB from Escherichia coli O157:H7 str. Sakai Escherichia coli O157:H7 str. Sakai 1789667 10955324 pO157p58 NP_052665.1 toxin B Virulence factor MUTATION: toxB mutants had attenuated adherence to Caco-2 cells [Ref6675:Tatsuno et al., 2001]. 11598035 2275 hlyA from Escherichia coli O157:H7 str. Sakai Escherichia coli O157:H7 str. Sakai 1789686 10955283 pO157p18 NP_052624.1 hemolysin A Virulence factor MUTATION: attenuated in zebra fish [Ref6655:Wiles et al., 2009]. 20019794 2276 hlyC from Escherichia coli O157:H7 str. Sakai Escherichia coli O157:H7 str. Sakai 1789689 10955282 pO157p17 NP_052623.1 hemolysin C Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells [Ref6656:Stanley et al., 1996]. COG2994O, under O: Posttranslational modification, protein turnover, chaperones 8793877 2277 espP from Escherichia coli O157:H7 str. Sakai Escherichia coli O157:H7 str. Sakai 1789732 10955344 pO157p78 NP_052685.1 EspP Virulence factor MUTATION: espP mutant is attenuated in calves [Ref6676:Dziva et al., 2007]. COG4625S, under S: Function unknown 17451446 2278 traJ from Escherichia coli APEC O1 Escherichia coli APEC O1 5616535 157418110 APECO1_O1CoBM27 YP_001481182.1 positive regulator of conjugative transfer; similar to AAO49515 Virulence factor MUTATION: traJ mutants of E. coli K1 are attenuated in rat pups and macrophages [Ref6671:Hill et al., 2004]. 14688129 2279 traT from Escherichia coli APEC O1 Escherichia coli APEC O1 5616563 157418138 APECO1_O1CoBM55 YP_001481210.1 surface exclusion and complement resistance protein; similar to AAT85681; identified by match to protein family HMM PF05818 Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 2280 tsh Escherichia coli APEC O1 5616581 157418156 APECO1_O1CoBM73 DQ381420 YP_001481228 Tsh Virulence factor MUTATION: tsh mutant is attenuated in chickens [Ref6677:Chen et al., 2008]. COG3468MU, under M: Cell wall/membrane/envelope biogenesis; U: Intracellular trafficking, secretion, and vesicular transport 18589815 2281 iroN from Escherichia coli APEC O1 Escherichia coli APEC O1 5616645 157418221 APECO1_O1CoBM137 YP_001481293.1 salmochelin siderophore system, salmochelin receptor; similar to AAS80269; identified by match to protein family HMM PF00593 Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum [Ref6232:Fischbach et al., 2006]. COG4771P, under P: Inorganic ion transport and metabolism 17060628 2282 iroD from Escherichia coli APEC O1 Escherichia coli APEC O1 5616647 157418223 APECO1_O1CoBM139 YP_001481295.1 salmochelin siderophore system, ferric enterochelin esterase; similar to AAS80267; identified by match to protein family HMM PF Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. COG2382P, under P: Inorganic ion transport and metabolism 18541653 2283 iroC from Escherichia coli APEC O1 Escherichia coli APEC O1 5616648 157418224 APECO1_O1CoBM140 YP_001481296.1 salmochelin siderophore system, ATP-binding cassette; similar to AAP41471; identified by match to protein family HMM PF00005 Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. COG1132V, under V: Defense mechanisms 18541653 2284 iroB from Escherichia coli APEC O1 Escherichia coli APEC O1 5616649 157418225 APECO1_O1CoBM141 YP_001481297.1 salmochelin siderophore system, glycosyltransferase; similar to AAS80265; identified by match to protein family HMM PF06722 Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. COG1819GC, under G: Carbohydrate transport and metabolism; C: Energy production and conversion 18541653 2285 iutA from Escherichia coli APEC O1 Escherichia coli APEC O1 5616676 157418252 APECO1_O1CoBM168 YP_001481324.1 aerobactin siderophore system, ferric aerobactin receptor protein; similar to ABA54744; identified by match to protein family HMM PF00593 Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG1629P, under P: Inorganic ion transport and metabolism 11553558 2286 iucD from Escherichia coli APEC O1 Escherichia coli APEC O1 5616677 157418253 APECO1_O1CoBM169 YP_001481325.1 aerobactin siderophore system, L-lysine 6-monooxygenase; similar to CAE55774 Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG3486Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 2287 iucC from Escherichia coli APEC O1 Escherichia coli APEC O1 5616678 157418254 APECO1_O1CoBM170 YP_001481326.1 aerobactin siderophore system, aerobactin biosynthesis protein; similar to ABA54742; identified by match to protein family HMMPFO4183 Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 2288 iucA from Escherichia coli APEC O1 Escherichia coli APEC O1 5616680 157418256 APECO1_O1CoBM172 YP_001481328.1 aerobactin siderophore system, aerobactin synthetase; similar to ABA54740; identified by match to protein family HMM PF04183 Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 2289 eatA from Escherichia coli E24377A Escherichia coli E24377A 5585855 157149562 EcE24377A_E0051 YP_001451588.1 secreted serine peptidase EatA Virulence factor MUTATION: interruption of the eatA gene retarded fluid accumulation in the rabbit ileal loop model, suggesting that this autotransporter contributes to the virulence of ETEC [Ref6230:Patel et al., 2004]. COG3468MU, under M: Cell wall/membrane/envelope biogenesis; U: Intracellular trafficking, secretion, and vesicular transport 14977988 2290 traJ from Escherichia coli UTI89 Escherichia coli UTI89 3996471 91206349 UTI89_P104 YP_538703.1 functional relaxosome complex initiator Virulence factor MUTATION: traJ mutants of E. coli K1 are attenuated in rat pups and macrophages [Ref6671:Hill et al., 2004]. 14688129 2291 traT from Escherichia coli UTI89 Escherichia coli UTI89 3996502 91206380 UTI89_P135 YP_538734.1 surface exclusion outer membrane protein Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 2292 espP from Escherichia coli O157:H7 str. EDL933 Escherichia coli O157:H7 str. EDL933 3683719 75994466 L7020 YP_325580.1 putative exoprotein-precursor Virulence factor MUTATION: espP mutant is attenuated in calves [Ref6676:Dziva et al., 2007]. COG4625S, under S: Function unknown 17451446 2293 EHEC-hlyC Escherichia coli O157:H7 str. EDL933 3683746 75994493 L7047 AF074613 YP_325607 hemolysin transport protein Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells [Ref6656:Stanley et al., 1996]. COG2994O, under O: Posttranslational modification, protein turnover, chaperones 8793877 2294 EHEC-hlyA Escherichia coli O157:H7 str. EDL933 3683747 75994494 L7048 AF074613 YP_325608 hemolysin toxin protein Virulence factor MUTATION: hlyA mutant is attenuated in calves [Ref6663:van et al., 2005]. 15731074 2295 traT Escherichia coli 2716459 41057009 pC15-1a_084 FJ449539 NP_957629 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 2296 tir Escherichia coli 2716469 41057025 pC15-1a_100 AF550679 NP_957645 hypothetical protein Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture [Ref6665:Schüller et al., 2007]. 17474908 2297 traT Escherichia coli 1446509 32470006 p165897_034 NP_862946.1 TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 2298 tir Escherichia coli 1446522 32470019 p165897_049 NP_862961.1 Tir Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture [Ref6665:Schüller et al., 2007]. 17474908 2299 traT from Escherichia coli E24377A Escherichia coli E24377A 5585733 157149414 EcE24377A_C0002 YP_001451443.1 TraT complement resistance protein Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 2300 eltA from Escherichia coli E24377A Escherichia coli E24377A 5585630 157149353 EcE24377A_F0020 YP_001451390.1 heat-labile enterotoxin A Virulence factor MUTATION: Deletion mutants of eltA and eltB had decreased levels of inducing cAMP secretion in an in vitro culture system, and had less adherence to IPEC-J2 and Caco-2 cells than the wild-type ETEC [Ref6678:Johnson et al., 2009]. 18978047 3368 rfaH from Escherichia coli SE11 Escherichia coli SE11 7002513 209921319 ECSE_4128 YP_002295403.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3369 tonB from Escherichia coli O157:H7 str. EC4115 Escherichia coli O157:H7 str. EC4115 6967035 209396499 ECH74115_1739 YP_002270184.1 transport protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001] COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3370 dsbA from Escherichia coli O157:H7 str. EC4115 Escherichia coli O157:H7 str. EC4115 6967123 209400816 ECH74115_5300 YP_002273372.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3371 dppA from Escherichia coli O157:H7 str. EC4115 Escherichia coli O157:H7 str. EC4115 6967851 209396782 ECH74115_4912 YP_002273027.1 dipeptide ABC transporter, periplasmic dipeptide-binding protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3372 chuA from Escherichia coli O157:H7 str. EC4115 Escherichia coli O157:H7 str. EC4115 6967853 209396568 ECH74115_4857 YP_002272975.1 outer membrane heme/hemoglobin receptor ChuA Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme [Ref6653:Torres and Payne, 1997]. COG4771P, under P: Inorganic ion transport and metabolism 9157252 3373 rfaH from Escherichia coli O157:H7 str. EC4115 Escherichia coli O157:H7 str. EC4115 6968671 209396563 ECH74115_5280 YP_002273359.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3374 ompA from Escherichia coli O157:H7 str. EC4115 Escherichia coli O157:H7 str. EC4115 6969157 209397392 ECH74115_1121 YP_002269625.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3375 fimH from Escherichia coli O157:H7 str. EC4115 Escherichia coli O157:H7 str. EC4115 6969617 209399868 ECH74115_5826 YP_002273845.1 protein FimH Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 3463 iroN from Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) 7379404 222104801 MM1_0014 YP_002539290.1 IroN, siderophore receptor Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum [Ref6232:Fischbach et al., 2006]. 17060628 3464 iroD from Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) 7379484 222104799 MM1_0011 YP_002539288.1 IroD, ferric enterochelin esterase Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. 18541653 3465 iutA from Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) 7379559 222104883 MM1_0096 YP_002539372.1 ferric aerobactin receptor precursor Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. 11553558 3466 iucC from Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) 7379561 222104885 MM1_0098 YP_002539374.1 aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. 2973985 3467 iucB from Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) 7379562 222104886 MM1_0099 YP_002539375.1 IucB Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. 2973985 3468 iucA from Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) Escherichia coli (strain: chi7122, serotype: O78:K80:H9, group: ExPEC, subgroup: APEC) 7379563 222104887 MM1_0100 YP_002539376.1 aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. 2973985 3469 iucA from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324506 221218526 pO103_24 YP_002527484.1 Aerobactin siderophore biosynthesis protein IucA Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. 2973985 3470 iucB from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324507 221218527 pO103_25 YP_002527485.1 Aerobactin siderophore biosynthesis protein IucB Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. 2973985 3471 iucC from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324508 221218528 pO103_26 YP_002527486.1 Aerobactin siderophore biosynthesis protein IucC Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. 2973985 3472 iucD from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324509 221218529 pO103_27 YP_002527487.1 Aerobactin siderophore L-lysine 6-monooxygenase IucD Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. 2973985 3473 iutA from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324510 221218530 pO103_28 YP_002527488.1 Aerobactin siderophore ferric receptor protein IutA Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. 11553558 3474 iroB from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324522 221218540 pO103_42 YP_002527498.1 Salmochelin siderophore glycosyltransferase IroB Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. 18541653 3475 iroC from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324523 221218541 pO103_43 YP_002527499.1 Salmochelin siderophore ATP-binding cassette IroC Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. 18541653 3476 iroD from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324524 221218542 pO103_44 YP_002527500.1 Salmochelin siderophore ferric enterochelin esterase IroD Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. 18541653 3477 iroN from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324526 221218544 pO103_46 YP_002527502.1 Salmochelin siderophore receptor protein IroN Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum [Ref6232:Fischbach et al., 2006]. 17060628 3478 traT from Escherichia coli (strain: APEC O103) Escherichia coli (strain: APEC O103) 7324607 221218625 pO103_127 YP_002527583.1 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 3479 traT from Escherichia coli (strain: H30, serovar: O26:H11) Escherichia coli (strain: H30, serovar: O26:H11) 7256873 219586125 pO26I_p078 YP_002456219.1 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 3480 espF from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7061130 215488961 E2348C_3930 YP_002331392.1 LEE-encoded effector EspF Virulence factor MUTATION: espF mutant of EPEC is deficient in its ability to disrupt electrical resistance across a polarized monolayer of intestinal epithelial cells, suggesting that EspF is an effector molecule required for disrupting intestinal epithelial barrier function [Ref6670:McNamara et al., 2001]. 11238563 3481 tonB from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7061491 215486487 E2348C_1379 YP_002328918.1 transport protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3482 rfaH from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7061758 215489181 E2348C_4154 YP_002331612.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3483 map from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7061805 215488973 E2348C_3942 YP_002331404.1 LEE-encoded effector Map Virulence factor MUTATION: map EPEC mutants have significantly reduced levels of invasion of HeLa cells [Ref6817:Jepson et al., 2003]. 14531893 3484 fliC from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7061913 215487128 E2348C_2041 YP_002329559.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 3485 fimH from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7062125 215489644 E2348C_4627 YP_002332075.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 3486 escN from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7062686 215488979 E2348C_3948 YP_002331410.1 translocator EscN Virulence factor MUTATION: deletion of escN abolished pathogenicity in infant rabbits compared to wild-type EHEC [Ref6664:Ritchie and Waldor, 2005]. COG1157NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 15731044 3487 ler from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7062691 215488999 E2348C_3968 YP_002331430.1 transcription regulator Ler Virulence factor MUTATION: ler mutants had less adhesion to HeLa cells, ler is a regulator of many virulence genes in the LEE PAI which are needed for adherence [Ref6818:Torres et al., 2008]. COG2916R, under R: General function prediction only 18794291 3488 espG from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7062692 215489001 E2348C_3970 YP_002331432.1 LEE-encoded effector EspG Virulence factor MUTATION: the inhibitory effect of the espG mutant on Cl– uptake in Caco-2 monolayers was significantly attenuated as compared with wild-type EPEC [Ref6819:Gill et al., 2007]. 17256057 3489 chuA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7062790 215488778 E2348C_3743 YP_002331209.1 outer membrane heme/hemoglobin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme [Ref6653:Torres and Payne, 1997]. COG4771P, under P: Inorganic ion transport and metabolism 9157252 3490 ompA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7063342 215486075 E2348C_0943 YP_002328506.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3491 ecpA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7063831 215485400 E2348C_0249 YP_002327831.1 fimbrillin precursor Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain [Ref6820:Rendón et al., 2007]. 17563352 3492 eaeA Escherichia coli O127:H6 str. E2348/69 7063868 215488970 E2348C_3939 YP_002331401.1 intimin EaeA Virulence factor MUTATION: attenuated in humans [Ref6666:Donnenberg et al., 1993]. 8376594 3493 espD from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7063878 215488966 E2348C_3935 YP_002331397.1 translocon EspD Virulence factor MUTATION: espD deletion mutant of the enterohemorrhagic E. coli (EHEC) strain EDL933 had impaired attachment to HeLa cells [Ref6668:Kresse et al., 1999]. COG5613S, under S: Function unknown 10456938 3494 espA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7063880 215488967 E2348C_3936 YP_002331398.1 translocon EspA Virulence factor MUTATION: deletion of espA in STEC abolished ability to attach to HeLa cells and induce actin rearrangements [Ref6667:Ebel et al., 1998]. 9786192 3495 dsbA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7063974 215489194 E2348C_4167 YP_002331625.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3496 espB from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7064346 215488965 E2348C_3934 YP_002331396.1 translocon EspB Virulence factor MUTATION: deletion mutant attenuated in humans compared to wild-type EPEC [Ref6669:Tacket et al., 2000]. 10816529 3497 espH from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7064347 215488975 E2348C_3944 YP_002331406.1 LEE-encoded effector EspH Virulence factor MUTATION: deletion mutant is attenuated in infant rabbits [Ref6664:Ritchie and Waldor, 2005]. 15731044 3498 tir from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7064348 215488972 E2348C_3941 YP_002331403.1 translocated intimin receptor Tir Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture [Ref6665:Schüller et al., 2007]. 17474908 3499 dppA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7065057 215488827 E2348C_3792 YP_002331258.1 dipeptide transporter Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3500 papG from Escherichia coli UMN026 Escherichia coli UMN026 7155270 218706490 ECUMN_3331 YP_002414009.1 Fimbrial PapG protein (P pilus class II adhesin) (PapGII) Virulence factor MUTATION: attenuated to human neutrophils in vitro [Ref6650:Tewari et al., 1994]. 7960108 3501 sat from Escherichia coli UMN026 Escherichia coli UMN026 7155292 218706521 ECUMN_3367 YP_002414040.1 Serine protease precursor (Secreted autotransporter toxin) Virulence factor MUTATION: Sat elicits cytopathic activity that damages host tissue and may increase the ability of E. coli to propagate [Ref6660:Guyer et al., 2002]. COG4625S, under S: Function unknown 12117966 3502 iutA from Escherichia coli UMN026 Escherichia coli UMN026 7155293 218706523 ECUMN_3371 YP_002414042.1 Ferric aerobactin receptor precursor Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG1629P, under P: Inorganic ion transport and metabolism 11553558 3503 dppA from Escherichia coli UMN026 Escherichia coli UMN026 7155420 218707178 ECUMN_4055 YP_002414697.1 dipeptide transporter ; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3504 aslA from Escherichia coli UMN026 Escherichia coli UMN026 7155811 218707435 ECUMN_4324 YP_002414954.1 arylsulfatase-like enzyme Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 [Ref6657:Hoffman et al., 2000]. COG3119P, under P: Inorganic ion transport and metabolism 10948126 3505 chuA from Escherichia coli UMN026 Escherichia coli UMN026 7155958 218707130 ECUMN_4000 YP_002414649.1 outer membrane hemin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme [Ref6653:Torres and Payne, 1997]. COG4771P, under P: Inorganic ion transport and metabolism 9157252 3506 fimH from Escherichia coli UMN026 Escherichia coli UMN026 7156362 218707987 ECUMN_4927 YP_002415506.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice in humans [Ref6644:Wiles et al., 2008]. 18482721 3507 iucA from Escherichia coli UMN026 Escherichia coli UMN026 7156875 218706527 ECUMN_3375 YP_002414046.1 aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3508 iucB from Escherichia coli UMN026 Escherichia coli UMN026 7156876 218706526 ECUMN_3374 YP_002414045.1 aerobactin siderophore biosynthesis protein, N(6)-hydroxylysine acetylase Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG1670J, under J: Translation, ribosomal structure and biogenesis 2973985 3509 iucC from Escherichia coli UMN026 Escherichia coli UMN026 7156877 218706525 ECUMN_3373 YP_002414044.1 Aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3510 iucD from Escherichia coli UMN026 Escherichia coli UMN026 7156878 218706524 ECUMN_3372 YP_002414043.1 L-lysine 6-monooxygenase (Lysine N(6)-hydroxylase) Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG3486Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3511 papA from Escherichia coli UMN026 Escherichia coli UMN026 7157388 218706498 ECUMN_3339 YP_002414017.1 P-fimbriae major subunit precursor Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence [Ref6648:Johnson, 1991]. COG3539NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 1672263 3512 rfaH from Escherichia coli UMN026 Escherichia coli UMN026 7158028 218707473 ECUMN_4366 YP_002414992.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice COG0250K, under K: Transcription 3513 ecpA from Escherichia coli UMN026 Escherichia coli UMN026 7158311 218703573 ECUMN_0327 YP_002411092.1 E. coli common pilus (ECP) Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain [Ref6820:Rendón et al., 2007]. 17563352 3514 ompA from Escherichia coli UMN026 Escherichia coli UMN026 7158500 218704380 ECUMN_1146 YP_002411899.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3515 tonB from Escherichia coli UMN026 Escherichia coli UMN026 7158576 218704775 ECUMN_1551 YP_002412294.1 transport protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3516 dsbA from Escherichia coli UMN026 Escherichia coli UMN026 7159290 218707486 ECUMN_4384 YP_002415005.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3517 ompA from Escherichia coli IAI39 Escherichia coli IAI39 7149956 218700524 ECIAI39_2190 YP_002408153.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3518 tia from Escherichia coli IAI39 Escherichia coli IAI39 7150281 218702439 ECIAI39_4192 YP_002410068.1 Tia invasion determinant Virulence factor MUTATION: tia deletion strains were noninvasive and lacked the ability to adhere to human ileocecal cells [Ref6649:Fleckenstein et al., 1996]. COG3637M, under M: Cell wall/membrane/envelope biogenesis 8675335 3519 chuA from Escherichia coli IAI39 Escherichia coli IAI39 7150945 218702265 ECIAI39_4002 YP_002409894.1 outer membrane hemin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme [Ref6653:Torres and Payne, 1997]. COG4771P, under P: Inorganic ion transport and metabolism 9157252 3520 dppA from Escherichia coli IAI39 Escherichia coli IAI39 7151167 218702310 ECIAI39_4053 YP_002409939.1 dipeptide transporter ; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3521 fimH from Escherichia coli IAI39 Escherichia coli IAI39 7151334 218703019 ECIAI39_4793 YP_002410648.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 3522 fliC from Escherichia coli IAI39 Escherichia coli IAI39 7151372 218699513 ECIAI39_1132 YP_002407142.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 3523 papA from Escherichia coli IAI39 Escherichia coli IAI39 7151788 0 ECIAI39_3404 - pseudo Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence [Ref6648:Johnson, 1991]. 1672263 3524 iucB from Escherichia coli IAI39 Escherichia coli IAI39 7151862 218701707 ECIAI39_3424 YP_002409336.1 aerobactin siderophore biosynthesis protein, N(6)-hydroxylysine acetylase Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG1670J, under J: Translation, ribosomal structure and biogenesis 2973985 3525 iucC from Escherichia coli IAI39 Escherichia coli IAI39 7151863 218701708 ECIAI39_3425 YP_002409337.1 Aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3526 iucD from Escherichia coli IAI39 Escherichia coli IAI39 7151864 218701709 ECIAI39_3426 YP_002409338.1 L-lysine 6-monooxygenase (Lysine N(6)-hydroxylase) Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG3486Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3527 iutA from Escherichia coli IAI39 Escherichia coli IAI39 7151865 218701710 ECIAI39_3427 YP_002409339.1 Ferric aerobactin receptor precursor Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG1629P, under P: Inorganic ion transport and metabolism 11553558 3528 papA from Escherichia coli IAI39 Escherichia coli IAI39 7152332 218702822 ECIAI39_4587 YP_002410451.1 major pilin protein PapA Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence [Ref6648:Johnson, 1991]. COG3539NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 1672263 3529 papG from Escherichia coli IAI39 Escherichia coli IAI39 7152338 218702830 ECIAI39_4595 YP_002410459.1 Fimbrial PapG protein (P pilus class II adhesin) (PapGII) Virulence factor MUTATION: attenuated to human neutrophils in vitro [Ref6650:Tewari et al., 1994]. 7960108 3530 tia from Escherichia coli IAI39 Escherichia coli IAI39 7152341 218702831 ECIAI39_4596 YP_002410460.1 Tia invasion determinant Virulence factor MUTATION: tia deletion strains were noninvasive and lacked the ability to adhere to human ileocecal cells [Ref6649:Fleckenstein et al., 1996]. COG3637M, under M: Cell wall/membrane/envelope biogenesis 8675335 3531 aslA from Escherichia coli IAI39 Escherichia coli IAI39 7152793 218701288 ECIAI39_2989 YP_002408917.1 arylsulfatase-like enzyme Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 [Ref6657:Hoffman et al., 2000]. COG3119P, under P: Inorganic ion transport and metabolism 10948126 3532 iucA from Escherichia coli IAI39 Escherichia coli IAI39 7152851 218701706 ECIAI39_3423 YP_002409335.1 aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3533 rfaH from Escherichia coli IAI39 Escherichia coli IAI39 7153071 218701455 ECIAI39_3155 YP_002409084.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3534 tonB from Escherichia coli IAI39 Escherichia coli IAI39 7153490 218699959 ECIAI39_1589 YP_002407588.1 transport protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3535 dsbA from Escherichia coli IAI39 Escherichia coli IAI39 7154218 218701440 ECIAI39_3140 YP_002409069.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3536 traJ from Escherichia coli UMN026 Escherichia coli UMN026 7144273 218692900 p1ECUMN_0115 YP_002406012.1 co,jugation transfer regulator protein Virulence factor MUTATION: traJ mutants of E. coli K1 are attenuated in rat pups and macrophages [Ref6671:Hill et al., 2004]. 14688129 3537 traT from Escherichia coli UMN026 Escherichia coli UMN026 7144350 218692867 p1ECUMN_0081 YP_002405979.1 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 3538 dppA from Escherichia coli 55989 Escherichia coli 55989 7145054 218697257 EC55989_3996 YP_002404924.1 dipeptide transporter ; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3539 iutA from Escherichia coli 55989 Escherichia coli 55989 7145114 218697849 EC55989_4641 YP_002405516.1 Ferric aerobactin receptor precursor Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG1629P, under P: Inorganic ion transport and metabolism 11553558 3540 iucA from Escherichia coli 55989 Escherichia coli 55989 7146638 218697853 EC55989_4645 YP_002405520.1 aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3541 iucB from Escherichia coli 55989 Escherichia coli 55989 7146639 218697852 EC55989_4644 YP_002405519.1 aerobactin siderophore biosynthesis protein, N(6)-hydroxylysine acetylase Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG1670J, under J: Translation, ribosomal structure and biogenesis 2973985 3542 iucC from Escherichia coli 55989 Escherichia coli 55989 7146640 218697851 EC55989_4643 YP_002405518.1 Aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3543 iucD from Escherichia coli 55989 Escherichia coli 55989 7146641 218697850 EC55989_4642 YP_002405517.1 L-lysine 6-monooxygenase (Lysine N(6)-hydroxylase) Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG3486Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3544 rfaH from Escherichia coli 55989 Escherichia coli 55989 7147756 218697561 EC55989_4317 YP_002405228.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3545 ecpA from Escherichia coli 55989 Escherichia coli 55989 7148058 218693755 EC55989_0297 YP_002401422.1 E. coli common pilus (ECP) Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain [Ref6820:Rendón et al., 2007]. 17563352 3546 ompA from Escherichia coli 55989 Escherichia coli 55989 7148255 218694432 EC55989_1006 YP_002402099.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3547 tonB from Escherichia coli 55989 Escherichia coli 55989 7148346 218694767 EC55989_1350 YP_002402434.1 transport protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3548 dsbA from Escherichia coli 55989 Escherichia coli 55989 7149102 218697574 EC55989_4336 YP_002405241.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3549 dppA from Escherichia coli ED1a Escherichia coli ED1a 7140515 218691829 ECED1_4223 YP_002400041.1 dipeptide transporter ; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3550 ecpA from Escherichia coli ED1a Escherichia coli ED1a 7141136 218688179 ECED1_0335 YP_002396391.1 E. coli common pilus (ECP) Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain [Ref6820:Rendón et al., 2007]. 17563352 3551 rfaH from Escherichia coli ED1a Escherichia coli ED1a 7141515 218692134 ECED1_4544 YP_002400346.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3552 tonB from Escherichia coli ED1a Escherichia coli ED1a 7141983 218689198 ECED1_1406 YP_002397410.1 transport protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3553 fliC from Escherichia coli ED1a Escherichia coli ED1a 7142129 218689916 ECED1_2188 YP_002398128.1 Flagellin (Phase 1-C flagellin) Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 3554 iucA from Escherichia coli ED1a Escherichia coli ED1a 7142472 218692458 ECED1_4905 YP_002400670.1 aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3555 iucB from Escherichia coli ED1a Escherichia coli ED1a 7142473 218692457 ECED1_4904 YP_002400669.1 aerobactin siderophore biosynthesis protein, N(6)-hydroxylysine acetylase Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG1670J, under J: Translation, ribosomal structure and biogenesis 2973985 3556 iucC from Escherichia coli ED1a Escherichia coli ED1a 7142474 218692456 ECED1_4903 YP_002400668.1 Aerobactin siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3557 iucD from Escherichia coli ED1a Escherichia coli ED1a 7142475 218692455 ECED1_4902 YP_002400667.1 L-lysine 6-monooxygenase (Lysine N(6)-hydroxylase) Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG3486Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3558 ompA from Escherichia coli ED1a Escherichia coli ED1a 7142711 218688793 ECED1_0980 YP_002397005.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3559 dsbA from Escherichia coli ED1a Escherichia coli ED1a 7142882 218692147 ECED1_4561 YP_002400359.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3560 iutA from Escherichia coli ED1a Escherichia coli ED1a 7143612 218692454 ECED1_4901 YP_002400666.1 Ferric aerobactin receptor precursor Virulence factor MUTATION: attenuated in mice. [Ref6234:Torres et al., 2001]. COG1629P, under P: Inorganic ion transport and metabolism 11553558 3561 iutA from Escherichia coli S88 Escherichia coli S88 7119366 218534486 pECS88_0009 YP_002401016.1 Ferric aerobactin receptor precusor IutA Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG1629P, under P: Inorganic ion transport and metabolism 11553558 3562 iroN from Escherichia coli S88 Escherichia coli S88 7119392 218534588 pECS88_0124 YP_002401118.1 outer membrane receptor FepA Virulence factor MUTATION: iroA gene cluster mutants were unable to resist Lcn2 in mouse serum [Ref6232:Fischbach et al., 2006]. COG4771P, under P: Inorganic ion transport and metabolism 17060628 3563 iroB from Escherichia coli S88 Escherichia coli S88 7119430 218534592 pECS88_0128 YP_002401122.1 IroB, putative glucosyltransferase Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. COG1819GC, under G: Carbohydrate transport and metabolism; C: Energy production and conversion 18541653 3564 iroC from Escherichia coli S88 Escherichia coli S88 7119431 218534591 pECS88_0127 YP_002401121.1 IroC, ATP binding cassette (ABC) transporter homolog Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. COG1132V, under V: Defense mechanisms 18541653 3565 iroD from Escherichia coli S88 Escherichia coli S88 7119432 218534590 pECS88_0126 YP_002401120.1 IroD, putative ferric enterochelin esterase Virulence factor MUTATION: attenuated in chicks [Ref6231:Caza et al., 2008]. COG2382P, under P: Inorganic ion transport and metabolism 18541653 3566 iucA from Escherichia coli S88 Escherichia coli S88 7119436 218534490 pECS88_0013 YP_002401020.1 Aerobactin siderophore biosynthesis protein IucA Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3567 iucB from Escherichia coli S88 Escherichia coli S88 7119437 218534489 pECS88_0012 YP_002401019.1 N(6)-hydroxylysine acetylase IucB Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG1670J, under J: Translation, ribosomal structure and biogenesis 2973985 3568 iucC from Escherichia coli S88 Escherichia coli S88 7119438 218534488 pECS88_0011 YP_002401018.1 Aerobactin siderophore biosynthesis protein IucC Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3569 iucD from Escherichia coli S88 Escherichia coli S88 7119439 218534487 pECS88_0010 YP_002401017.1 L-lysine 6-monooxigenase IucD Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG3486Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3570 traJ from Escherichia coli S88 Escherichia coli S88 7119465 218534534 pECS88_0064 YP_002401064.1 conjugal transfer transcriptional regulator TraJ Virulence factor MUTATION: traJ mutants of E. coli K1 are attenuated in rat pups and macrophages [Ref6671:Hill et al., 2004]. 14688129 3571 traT from Escherichia coli S88 Escherichia coli S88 7119470 218534560 pECS88_0092 YP_002401090.1 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 3572 fimH from Escherichia coli S88 Escherichia coli S88 7129499 218561496 ECS88_4938 YP_002394409.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 3573 aslA from Escherichia coli S88 Escherichia coli S88 7129944 218560864 ECS88_4222 YP_002393777.1 arylsulfatase-like enzyme Virulence factor MUTATION: disruption mutant was less invasive in an in vitro HBMEC invasion assay than the wild-type E. coli K1 [Ref6657:Hoffman et al., 2000]. COG3119P, under P: Inorganic ion transport and metabolism 10948126 3574 ompA from Escherichia coli S88 Escherichia coli S88 7130155 218557855 ECS88_0978 YP_002390768.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3575 dppA from Escherichia coli S88 Escherichia coli S88 7130233 218560622 ECS88_3964 YP_002393535.1 dipeptide transporter ; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3576 ecpA from Escherichia coli S88 Escherichia coli S88 7130299 218557210 ECS88_0290 YP_002390123.1 E. coli common pilus (ECP) Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain [Ref6820:Rendón et al., 2007]. 17563352 3577 dsbA from Escherichia coli S88 Escherichia coli S88 7130870 218560936 ECS88_4310 YP_002393849.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3578 rfaH from Escherichia coli S88 Escherichia coli S88 7131265 218560923 ECS88_4290 YP_002393836.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3579 tonB from Escherichia coli S88 Escherichia coli S88 7131480 218558181 ECS88_1322 YP_002391094.1 transport protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3580 chuA from Escherichia coli S88 Escherichia coli S88 7131907 218560576 ECS88_3913 YP_002393489.1 outer membrane hemin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme [Ref6653:Torres and Payne, 1997]. COG4771P, under P: Inorganic ion transport and metabolism 9157252 3581 FliC from Escherichia coli S88 Escherichia coli S88 7132788 218558781 ECS88_1977 YP_002391694.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 3582 papA from Escherichia coli S88 Escherichia coli S88 7133169 218559971 ECS88_3265 YP_002392884.1 major pilin protein PapA Virulence factor MUTATION: mutant papA in wild-type smooth strains do not mediate Gal-Gal adherence [Ref6648:Johnson, 1991]. COG3539NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 1672263 3583 tia from Escherichia coli S88 Escherichia coli S88 7133342 218559962 ECS88_3256 YP_002392875.1 Tia invasion determinant Virulence factor MUTATION: tia deletion strains were noninvasive and lacked the ability to adhere to human ileocecal cells [Ref6649:Fleckenstein et al., 1996]. COG3637M, under M: Cell wall/membrane/envelope biogenesis 8675335 3584 papG from Escherichia coli S88 Escherichia coli S88 7133471 218559963 ECS88_3257 YP_002392876.1 Fimbrial PapG protein (P pilus class II adhesin) (PapGII) Virulence factor MUTATION: attenuated to human neutrophils in vitro [Ref6650:Tewari et al., 1994]. 7960108 3585 ompA from Escherichia coli IAI1 Escherichia coli IAI1 7124740 218553544 ECIAI1_0998 YP_002386457.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3586 dppA from Escherichia coli IAI1 Escherichia coli IAI1 7125629 218556099 ECIAI1_3703 YP_002389012.1 dipeptide transporter ; periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3587 rfaH from Escherichia coli IAI1 Escherichia coli IAI1 7126217 218556406 ECIAI1_4035 YP_002389320.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3588 ecpA from Escherichia coli IAI1 Escherichia coli IAI1 7126700 218552859 ECIAI1_0294 YP_002385772.1 E. coli common pilus (ECP) Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain [Ref6820:Rendón et al., 2007]. 17563352 3589 fliC from Escherichia coli IAI1 Escherichia coli IAI1 7126825 218554508 ECIAI1_2009 YP_002387421.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 3590 fimH from Escherichia coli IAI1 Escherichia coli IAI1 7127116 218556851 ECIAI1_4536 YP_002389765.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 3591 tonB from Escherichia coli IAI1 Escherichia coli IAI1 7127821 218553804 ECIAI1_1272 YP_002386717.1 transport protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3592 dsbA from Escherichia coli IAI1 Escherichia coli IAI1 7128194 218556419 ECIAI1_4059 YP_002389333.1 periplasmic protein disulfide isomerase I Virulence factor, Protective antigen MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3593 bfpE from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056322 215276200 E2348_P1_009 YP_002332163.1 transmembrane protein Virulence factor MUTATION: bfpE mutant strain of EPEC fails to form detectable BFP and fails to carry out autoaggregation and localized adherence to HEp-2 epithelial cells in tissue culture [Ref6237:Blank and Donnenberg, 2001]. COG1459NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 11443077 3594 bfpF from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056323 215276201 E2348_P1_010 YP_002332164.1 nucleotide binding protein Virulence factor MUTATION: bfpF mutant colonized the human intestine but was about 200-fold less virulent than the wild-type EPEC [Ref6235:Bieber et al., 1998]. COG2805NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 9641917 3595 bfpP from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056324 215276202 E2348_P1_011 YP_002332165.1 prepilin peptidase Virulence factor MUTATION: mutation in bfpP blocks BFP biogenesis and localized adherence to host cells by EPEC [Ref6238:Anantha et al., 2000]. COG1989NOU, under N: Cell motility; O: Posttranslational modification, protein turnover, chaperones; U: Intracellular trafficking, secretion, and vesicular transport 10762251 3596 bfpB from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056325 215276196 E2348_P1_005 YP_002332159.1 lipoprotein Virulence factor MUTATION: bfpB mutant EPEC is unable to form BFP which is a major virulence factor of EPEC [Ref6236:Ramer et al., 1996]. COG1450NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 8932312 3597 bfpA from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056343 215276194 E2348_P1_003 YP_002332157.1 major pilin structural unit bundlin Virulence factor MUTATION: bfpA deletion mutant of EPEC was less virulent in humans than the wild-type EPEC [Ref6235:Bieber et al., 1998]. 9641917 3598 traT from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056350 215276226 E2348_P1_046 YP_002332189.1 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 3599 traJ from Escherichia coli O127:H6 str. E2348/69 Escherichia coli O127:H6 str. E2348/69 7056370 215276255 E2348_P1_075 YP_002332218.1 transcriptional activator Virulence factor MUTATION: traJ mutants of E. coli K1 are attenuated in rat pups and macrophages [Ref6671:Hill et al., 2004]. 14688129 3678 toxB from Escherichia coli O26:H- Escherichia coli O26:H- 8715786 284000161 pO26CRL_0032 YP_003377848.1 toxin B Virulence factor MUTATION: toxB mutants had attenuated adherence to Caco-2 cells [Ref6675:Tatsuno et al., 2001]. 11598035 3679 hlyC from Escherichia coli O26:H- Escherichia coli O26:H- 8715862 284000229 pO26CRL_0108 YP_003377916.1 hemolysin C Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells [Ref6656:Stanley et al., 1996]. 8793877 3680 hlyA from Escherichia coli O26:H- Escherichia coli O26:H- 8715863 284000230 pO26CRL_0109 YP_003377917.1 hemolysin A Virulence factor MUTATION: attenuated in zebra fish [Ref6655:Wiles et al., 2009]. 20019794 3681 ompA from Escherichia coli B str. REL606 Escherichia coli B str. REL606 8175755 254161071 ECB_00961 YP_003044179.1 outer membrane protein A (3a;II*;G;d) Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3682 dppA from Escherichia coli B str. REL606 Escherichia coli B str. REL606 8177616 254163468 ECB_03395 YP_003046576.1 dipeptide transporter Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3683 rfaH from Escherichia coli B str. REL606 Escherichia coli B str. REL606 8177876 254163799 ECB_03733 YP_003046907.1 transcriptional activator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3684 dsbA from Escherichia coli B str. REL606 Escherichia coli B str. REL606 8177889 254163812 ECB_03746 YP_003046920.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3685 fimH from Escherichia coli B str. REL606 Escherichia coli B str. REL606 8178231 254164241 ECB_04189 YP_003047351.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 3686 tonB from Escherichia coli B str. REL606 Escherichia coli B str. REL606 8178456 254161331 ECB_01226 YP_003044439.1 membrane spanning protein in TonB-ExbB-ExbD complex Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3687 traT from Escherichia coli (strain: EC14, pathovar: UPEC, nat-host: Homo sapiens) Escherichia coli (strain: EC14, pathovar: UPEC, nat-host: Homo sapiens) 8381950 256855359 pEC14_110 YP_003162603.1 IncF plasmid conjugative transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 3688 tir from Escherichia coli (strain: A, other: UK epidemic CTX-M-producing strain (PFGE-defined); MLST-defined ST131 linea Escherichia coli (strain: A, other: UK epidemic CTX-M-producing strain (PFGE-defined); MLST-defined 8319255 256367770 pEK499_p062 YP_003108327.1 inner membrane protein Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture [Ref6665:Schüller et al., 2007]. 17474908 3689 tir from Escherichia coli (strain: D, other: UK epidemic CTX-M-producing strain (PFGE-defined); MLST-defined ST131 linea Escherichia coli (strain: D, other: UK epidemic CTX-M-producing strain (PFGE-defined); MLST-defined 8319157 256367694 pEK516_p77 YP_003108263.1 Tir Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture [Ref6665:Schüller et al., 2007]. 17474908 3690 eltA from Escherichia coli ETEC H10407 Escherichia coli ETEC H10407 8571459 268507198 pEntH10407_p39 YP_003293997.1 heat-labile enterotoxin A subunit Virulence factor MUTATION: Deletion mutants of eltA and eltB had decreased levels of inducing cAMP secretion in an in vitro culture system, and had less adherence to IPEC-J2 and Caco-2 cells than the wild-type ETEC [Ref6678:Johnson et al., 2009]. 18978047 3691 eltB Escherichia coli ETEC H10407 8571464 268507197 pEntH10407_p38 AP010910 YP_003293996 heat-labile enterotoxin B subunit Virulence factor MUTATION: Deletion mutants of eltA and eltB had decreased levels of inducing cAMP secretion in an in vitro culture system, and had less adherence to IPEC-J2 and Caco-2 cells than the wild-type ETEC [Ref6678:Johnson et al., 2009]. 18978047 3692 hcpA Escherichia coli O157:H7 str. TW14359 8214675 254791243 ECSP_0109 CP001368 YP_003076080 putative major pilin subunit Virulence factor MUTATION: hcpA deletion mutant had attenuated invasion of cultured epithelial cells compared to wild-type EHEC [Ref6836:Xicohtencatl-Cortes et al., 2009]. COG4969NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 18952791 3693 ecpA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8214896 254791452 ECSP_0330 YP_003076289.1 Mat fimbrillin Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain [Ref6820:Rendón et al., 2007]. 17563352 3694 ompA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8215629 254792157 ECSP_1063 YP_003076994.1 outer membrane protein A (3a;II*;G;d) Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3695 espK Escherichia coli O157:H7 str. TW14359 8216050 254792564 ECSP_1484 CP001368 YP_003077401 non-LEE-encoded type III secreted effector Virulence factor MUTATION: espK mutant attenuated in calves [Ref6837:Vlisidou et al., 2006]. 16958848 3696 tonB from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8216210 254792722 ECSP_1644 YP_003077559.1 TonB protein Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3697 nleA Escherichia coli O157:H7 str. TW14359 8216268 254792777 ECSP_1702 CP001368 YP_003077614 non-LEE-encoded type III secreted effector Virulence factor MUTATION: nelA deletion mutant attenuated in mice [Ref6838:Gruenheid et al., 2004]. 14982621 3698 fliC from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8217094 254793574 ECSP_2528 YP_003078411.1 flagellar filament structural protein (flagellin) Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 3699 stx2B from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8217818 254794270 ECSP_3252 CP001368 YP_003079107 shiga toxin II subunit B Virulence factor TOXIN: Stx induces apoptosis in HeLa cells [Ref6661:Cherla et al., 2003]. 14638419 3700 chuA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219055 254795452 ECSP_4489 YP_003080289.1 outer membrane heme/hemoglobin receptor Virulence factor MUTATION: chuA mutant unable to grow on iron-depleted medium containing heme [Ref6653:Torres and Payne, 1997]. COG4771P, under P: Inorganic ion transport and metabolism 9157252 3701 dppA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219101 254795498 ECSP_4535 YP_003080335.1 dipeptide transporter, periplasmic-binding component of ABC superfamily Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3702 espF from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219231 254795625 ECSP_4665 YP_003080462.1 LEE-encoded type III secreted effector Virulence factor MUTATION: espF mutant of EPEC is deficient in its ability to disrupt electrical resistance across a polarized monolayer of intestinal epithelial cells, suggesting that EspF is an effector molecule required for disrupting intestinal epithelial barrier function [Ref6670:McNamara et al., 2001]. 11238563 3703 espB from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219235 254795629 ECSP_4669 YP_003080466.1 secreted protein EspB Virulence factor MUTATION: deletion mutant attenuated in humans compared to wild-type EPEC [Ref6669:Tacket et al., 2000]. 10816529 3704 espD from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219236 254795630 ECSP_4670 YP_003080467.1 secreted protein EspD Virulence factor MUTATION: espD deletion mutant of the enterohemorrhagic E. coli (EHEC) strain EDL933 had impaired attachment to HeLa cells [Ref6668:Kresse et al., 1999]. COG5613S, under S: Function unknown 10456938 3705 espA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219237 254795631 ECSP_4671 YP_003080468.1 secreted protein EspA Virulence factor MUTATION: deletion of espA in STEC abolished ability to attach to HeLa cells and induce actin rearrangements [Ref6667:Ebel et al., 1998]. 9786192 3706 eae from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219240 254795634 ECSP_4674 YP_003080471.1 intimin adherence protein Virulence factor MUTATION: attenuated in humans [Ref6666:Donnenberg et al., 1993]. 8376594 3707 tir from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219242 254795636 ECSP_4676 YP_003080473.1 translocated intimin receptor protein Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture [Ref6665:Schüller et al., 2007]. 17474908 3708 espH from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219245 254795639 ECSP_4679 YP_003080476.1 LEE-encoded type III secreted effector Virulence factor MUTATION: deletion mutant is attenuated in infant rabbits [Ref6664:Ritchie and Waldor, 2005]. 15731044 3709 escN from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219249 254795643 ECSP_4683 YP_003080480.1 LEE-encoded type III secretion system factor Virulence factor MUTATION: deletion of escN abolished pathogenicity in infant rabbits compared to wild-type EHEC [Ref6664:Ritchie and Waldor, 2005]. COG1157NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 15731044 3710 ler from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219269 254795663 ECSP_4703 YP_003080500.1 locus of enterocyte effacement (LEE)-encoded regulator Virulence factor MUTATION: ler mutants had less adhesion to HeLa cells, ler is a regulator of many virulence genes in the LEE PAI which are needed for adherence [Ref6818:Torres et al., 2008]. COG2916R, under R: General function prediction only 18794291 3711 espG from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219270 254795664 ECSP_4704 YP_003080501.1 LEE-encoded type III secreted effector Virulence factor MUTATION: the inhibitory effect of the espG mutant on Cl– uptake in Caco-2 monolayers was significantly attenuated as compared with wild-type EPEC [Ref6819:Gill et al., 2007]. 17256057 3712 rfaH from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219461 254795838 ECSP_4895 YP_003080675.1 DNA-binding transcriptional antiterminator Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3713 dsbA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219479 254795851 ECSP_4913 YP_003080688.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3714 fimH from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8219970 254796322 ECSP_5404 YP_003081159.1 minor fimbrial subunit, D-mannose specific adhesin Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 3715 iucA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8484733 260867380 ECO111_1293 YP_003233782.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3716 espG from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8485562 260869665 ECO111_3731 YP_003236067.1 T3SS secreted effector EspG Virulence factor MUTATION: the inhibitory effect of the espG mutant on Cl– uptake in Caco-2 monolayers was significantly attenuated in as compared with wild-type EPEC 3717 espF from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8485564 260869668 ECO111_3734 YP_003236070.1 T3SS secreted effector EspF Virulence factor MUTATION: espF mutant of EPEC is deficient in its ability to disrupt electrical resistance across a polarized monolayer of intestinal epithelial cells, suggesting that EspF is an effector molecule required for disrupting intestinal epithelial barrier function [Ref6670:McNamara et al., 2001]. 11238563 3718 escN from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8485567 260869686 ECO111_3752 YP_003236088.1 translocator EscN Virulence factor MUTATION: deletion of escN abolished pathogenicity in infant rabbits compared to wild-type EHEC [Ref6664:Ritchie and Waldor, 2005]. COG1157NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 15731044 3719 ler from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8485572 260869706 ECO111_3772 YP_003236108.1 transcription regulator Ler Virulence factor MUTATION: ler mutants had less adhesion to HeLa cells, ler is a regulator of many virulence genes in the LEE PAI which are needed for adherence [Ref6818:Torres et al., 2008]. COG2916R, under R: General function prediction only 18794291 3720 efa1/lifA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8485768 260870884 ECO111_5002 YP_003237286.1 Efa1/LifA-like protein Virulence factor MUTATION: deletion mutant lacks the ability to inhibit lymphokine production and lymphocyte proliferation compared to wild type EPEC [Ref6839:Klapproth et al., 2000]. 10722613 3721 espB from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486116 260869672 ECO111_3738 YP_003236074.1 translocon EspB Virulence factor MUTATION: deletion mutant attenuated in humans compared to wild-type EPEC [Ref6669:Tacket et al., 2000]. 10816529 3722 espH from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486117 260869682 ECO111_3748 YP_003236084.1 T3SS secreted effector EspH Virulence factor MUTATION: deletion mutant is attenuated in infant rabbits [Ref6664:Ritchie and Waldor, 2005]. 15731044 3723 tir from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486118 260869679 ECO111_3745 YP_003236081.1 putative translocated intimin receptor Tir Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture [Ref6665:Schüller et al., 2007]. 17474908 3724 dppA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486355 260870271 ECO111_4360 YP_003236673.1 dipeptide transport protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3725 eae from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486421 260869677 ECO111_3743 YP_003236079.1 theta intimin Virulence factor MUTATION: attenuated in humans [Ref6666:Donnenberg et al., 1993]. 8376594 3726 espD from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486431 260869673 ECO111_3739 YP_003236075.1 translocon EspD Virulence factor MUTATION: espD deletion mutant of the enterohemorrhagic E. coli (EHEC) strain EDL933 had impaired attachment to HeLa cells [Ref6668:Kresse et al., 1999]. COG5613S, under S: Function unknown 10456938 3727 espA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486432 260869674 ECO111_3740 YP_003236076.1 translocon EspA Virulence factor MUTATION: deletion of espA in STEC abolished ability to attach to HeLa cells and induce actin rearrangements [Ref6667:Ebel et al., 1998]. 9786192 3728 fimH from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486516 260871042 ECO111_5176 YP_003237444.1 minor component FimH of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 3729 fliC from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8486550 260868515 ECO111_2503 YP_003234917.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 3730 iucB from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8487016 260867381 ECO111_1294 YP_003233783.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG1670J, under J: Translation, ribosomal structure and biogenesis 2973985 3731 iucC from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8487017 260867382 ECO111_1295 YP_003233784.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3732 iucD from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8487018 260867383 ECO111_1296 YP_003233785.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG3486Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3733 iutA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8487019 260867384 ECO111_1297 YP_003233786.1 putative ferric siderophore receptor Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG1629P, under P: Inorganic ion transport and metabolism 11553558 3734 rfaH from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8488159 260870564 ECO111_4668 YP_003236966.1 DNA-binding transcriptional antiterminator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3735 map from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8488207 260869680 ECO111_3746 YP_003236082.1 T3SS secreted effector Map Virulence factor MUTATION: map EPEC mutants have significantly reduced levels of invasion of HeLa cells [Ref6817:Jepson et al., 2003]. 14531893 3736 ompA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8488643 260867129 ECO111_1025 YP_003233531.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3737 tonB from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8488732 260868022 ECO111_1986 YP_003234424.1 membrane spanning protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3738 dsbA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8489430 260870577 ECO111_4681 YP_003236979.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3739 iucA from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8479008 260854496 ECO26_1345 YP_003228387.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3740 espG from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480236 260858241 ECO26_5250 YP_003232132.1 T3SS secreted effector EspG Virulence factor MUTATION: the inhibitory effect of the espG mutant on Cl– uptake in Caco-2 monolayers was significantly attenuated as compared with wild-type EPEC [Ref6819:Gill et al., 2007]. 17256057 3741 espF from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480244 260858280 ECO26_5289 YP_003232171.1 T3SS secreted effector EspF Virulence factor MUTATION: espF mutant of EPEC is deficient in its ability to disrupt electrical resistance across a polarized monolayer of intestinal epithelial cells, suggesting that EspF is an effector molecule required for disrupting intestinal epithelial barrier function [Ref6670:McNamara et al., 2001]. 11238563 3742 efa1/lifA fromEscherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480245 260858281 ECO26_5291 YP_003232172.1 Efa1/LifA-like protein Virulence factor MUTATION: deletion mutant lacks the ability to inhibit lymphokine production and lymphocyte proliferation compared to wild type EPEC [Ref6839:Klapproth et al., 2000]. 10722613 3743 map from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480654 260858268 ECO26_5277 YP_003232159.1 T3SS secreted effector Map Virulence factor MUTATION: map EPEC mutants have significantly reduced levels of invasion of HeLa cells [Ref6817:Jepson et al., 2003]. 14531893 3744 eae from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480655 260858271 ECO26_5280 YP_003232162.1 intimin Virulence factor MUTATION: attenuated in humans [Ref6666:Donnenberg et al., 1993]. 8376594 3745 dppA from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480894 260857652 ECO26_4638 YP_003231543.1 dipeptide transport protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3746 matB/ecpA from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480920 260853523 ECO26_0328 YP_003227414.1 putative fimbrillin precursor Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain [Ref6820:Rendón et al., 2007]. 17563352 3747 escN from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480968 260858262 ECO26_5271 YP_003232153.1 T3SS structure protein EscN Virulence factor MUTATION: deletion of escN abolished pathogenicity in infant rabbits compared to wild-type EHEC [Ref6664:Ritchie and Waldor, 2005]. COG1157NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 15731044 3748 espD from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480969 260858275 ECO26_5284 YP_003232166.1 translocator EspD Virulence factor MUTATION: espD deletion mutant of the enterohemorrhagic E. coli (EHEC) strain EDL933 had impaired attachment to HeLa cells [Ref6668:Kresse et al., 1999]. COG5613S, under S: Function unknown 10456938 3749 espB from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480971 260858276 ECO26_5285 YP_003232167.1 translocator EspB Virulence factor MUTATION: deletion mutant attenuated in humans compared to wild-type EPEC [Ref6669:Tacket et al., 2000]. 10816529 3750 ler from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8480973 260858242 ECO26_5251 YP_003232133.1 transcription regulator Ler Virulence factor MUTATION: ler mutants had less adhesion to HeLa cells, ler is a regulator of many virulence genes in the LEE PAI which are needed for adherence [Ref6818:Torres et al., 2008]. COG2916R, under R: General function prediction only 18794291 3751 fimH from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8481056 260858488 ECO26_5517 YP_003232379.1 minor component FimH of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 3752 fliC from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8481090 260855903 ECO26_2815 YP_003229794.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 3753 iucB from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8481558 260854497 ECO26_1346 YP_003228388.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG1670J, under J: Translation, ribosomal structure and biogenesis 2973985 3754 iucC from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8481559 260854498 ECO26_1347 YP_003228389.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG4264Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3755 iucD from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8481560 260854499 ECO26_1348 YP_003228390.1 siderophore biosynthesis protein Virulence factor MUTATION: attenuated in mice [Ref6233:de and Martinez, 1988]. COG3486Q, under Q: Secondary metabolites biosynthesis, transport and catabolism 2973985 3756 iutA from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8481561 260854500 ECO26_1349 YP_003228391.1 putative ferric siderophore receptor Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG1629P, under P: Inorganic ion transport and metabolism 11553558 3757 tir from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8481750 260858269 ECO26_5278 YP_003232160.1 translocated intimin receptor Tir Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture [Ref6665:Schüller et al., 2007]. 17474908 3758 espA from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8482581 260858274 ECO26_5283 YP_003232165.1 translocator EspA Virulence factor MUTATION: deletion of espA in STEC abolished ability to attach to HeLa cells and induce actin rearrangements [Ref6667:Ebel et al., 1998]. 9786192 3759 espH from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8482582 260858266 ECO26_5275 YP_003232157.1 T3SS secreted effector EspH Virulence factor MUTATION: deletion mutant is attenuated in infant rabbits [Ref6664:Ritchie and Waldor, 2005]. 15731044 3760 rfaH from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8482829 260857754 ECO26_4745 YP_003231645.1 DNA-binding transcriptional antiterminator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3761 ompA from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8483180 260854249 ECO26_1084 YP_003228140.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3762 tonB from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8483275 260854911 ECO26_1764 YP_003228802.1 membrane spanning protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3763 dsbA from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8483965 260857741 ECO26_4732 YP_003231632.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3764 efa1/lifA from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8474155 260845650 ECO103_3564 YP_003223428.1 Efa1/LifA-like protein Virulence factor MUTATION: deletion mutant lacks the ability to inhibit lymphokine production and lymphocyte proliferation compared to wild type EPEC [Ref6839:Klapproth et al., 2000]. 10722613 3765 espF from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8474190 260845679 ECO103_3600 YP_003223457.1 T3SS secreted effector EspF Virulence factor MUTATION: espF mutant of EPEC is deficient in its ability to disrupt electrical resistance across a polarized monolayer of intestinal epithelial cells, suggesting that EspF is an effector molecule required for disrupting intestinal epithelial barrier function [Ref6670:McNamara et al., 2001]. 11238563 3766 escN from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8474193 260845697 ECO103_3618 YP_003223475.1 translocator EscN Virulence factor MUTATION: deletion of escN abolished pathogenicity in infant rabbits compared to wild-type EHEC [Ref6664:Ritchie and Waldor, 2005]. COG1157NU, under N: Cell motility; U: Intracellular trafficking, secretion, and vesicular transport 15731044 3767 ler from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8474199 260845717 ECO103_3638 YP_003223495.1 transcription regulator Ler Virulence factor MUTATION: ler mutants had less adhesion to HeLa cells, ler is a regulator of many virulence genes in the LEE PAI which are needed for adherence [Ref6818:Torres et al., 2008]. COG2916R, under R: General function prediction only 18794291 3768 espB from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8474872 260845683 ECO103_3604 YP_003223461.1 translocon EspB Virulence factor MUTATION: deletion mutant attenuated in humans compared to wild-type EPEC [Ref6669:Tacket et al., 2000]. 10816529 3769 espH from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8474873 260845693 ECO103_3614 YP_003223471.1 T3SS secreted effector EspH Virulence factor MUTATION: deletion mutant is attenuated in infant rabbits [Ref6664:Ritchie and Waldor, 2005]. 15731044 3770 tir from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8474874 260845690 ECO103_3611 YP_003223468.1 translocated intimin receptor Tir Virulence factor MUTATION: tir deletion strains are unable to adhere to tissue in an in vitro organ culture [Ref6665:Schüller et al., 2007]. 17474908 3771 dppA from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8475113 260846337 ECO103_4276 YP_003224115.1 dipeptide transport protein Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3772 matB/ecpA from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8475139 260842499 ECO103_0270 YP_003220277.1 putative fimbrillin precursor Virulence factor MUTATION: ecpA mutant had attenuated adherence to HEp-2 cells compared to the parental EHEC strain [Ref6820:Rendón et al., 2007]. 17563352 3773 eae from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8475178 260845688 ECO103_3609 YP_003223466.1 intimin epsilon Virulence factor MUTATION: attenuated in humans [Ref6666:Donnenberg et al., 1993]. 8376594 3774 espD from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8475188 260845684 ECO103_3605 YP_003223462.1 translocon EspD Virulence factor MUTATION: espD deletion mutant of the enterohemorrhagic E. coli (EHEC) strain EDL933 had impaired attachment to HeLa cells [Ref6668:Kresse et al., 1999]. COG5613S, under S: Function unknown 10456938 3775 espA from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8475189 260845685 ECO103_3606 YP_003223463.1 translocon EspA Virulence factor MUTATION: deletion of espA in STEC abolished ability to attach to HeLa cells and induce actin rearrangements [Ref6667:Ebel et al., 1998]. 9786192 3776 fimH from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8475277 260847136 ECO103_5101 YP_003224914.1 minor component FimH of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 3777 fliC from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8475311 260844327 ECO103_2179 YP_003222105.1 flagellin Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 3778 espG from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8475820 260845718 ECO103_3639 YP_003223496.1 T3SS secreted effector EspG Virulence factor MUTATION: the inhibitory effect of the espG mutant on Cl– uptake in Caco-2 monolayers was significantly attenuated as compared with wild-type EPEC [Ref6819:Gill et al., 2007]. 17256057 3779 map from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8476939 260845691 ECO103_3612 YP_003223469.1 T3SS secreted effector Map Virulence factor MUTATION: map EPEC mutants have significantly reduced levels of invasion of HeLa cells [Ref6817:Jepson et al., 2003]. 14531893 3780 rfaH from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8477018 260846384 ECO103_4323 YP_003224162.1 DNA-binding transcriptional antiterminator RfaH Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3781 ompA from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8477375 260843206 ECO103_1003 YP_003220984.1 outer membrane protein A Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3782 tonB from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8477474 260843543 ECO103_1353 YP_003221321.1 membrane spanning protein TonB Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3783 dsbA from Escherichia coli O103:H2 str. 12009 Escherichia coli O103:H2 str. 12009 8478162 260846371 ECO103_4310 YP_003224149.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 3784 hlyA from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8473051 260763804 ECO26_p1-02 YP_003237843.1 hemolysin A Virulence factor MUTATION: attenuated in zebra fish [Ref6655:Wiles et al., 2009]. 20019794 3785 hlyC from Escherichia coli O26:H11 str. 11368 Escherichia coli O26:H11 str. 11368 8473053 260763803 ECO26_p1-01 YP_003237842.1 hemolysin C Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells [Ref6656:Stanley et al., 1996]. COG2994O, under O: Posttranslational modification, protein turnover, chaperones 8793877 3786 hlyA from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8470732 260751837 ECO111_p3-02 YP_003237751.1 hemolysin A Virulence factor MUTATION: attenuated in zebra fish [Ref6655:Wiles et al., 2009]. 20019794 3787 hlyC from Escherichia coli O111:H- str. 11128 Escherichia coli O111:H- str. 11128 8470734 260751836 ECO111_p3-01 YP_003237750.1 hemolysin C Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells [Ref6656:Stanley et al., 1996]. COG2994O, under O: Posttranslational modification, protein turnover, chaperones 8793877 3788 hylC Escherichia coli O103:H2 str. 12009 8470589 260718958 ECO103_p32 AP010959 YP_003225099 hemolysin C Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells [Ref6656:Stanley et al., 1996]. COG2994O, under O: Posttranslational modification, protein turnover, chaperones 8793877 3789 hlyC from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8206889 254667465 ECSP_6017 YP_003082151.1 hemolysin transport protein Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells [Ref6656:Stanley et al., 1996]. COG2994O, under O: Posttranslational modification, protein turnover, chaperones 8793877 3790 hlyA from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8206890 254667466 ECSP_6018 YP_003082152.1 hemolysin toxin protein Virulence factor MUTATION: attenuated in zebra fish [Ref6655:Wiles et al., 2009]. 20019794 3791 espP from Escherichia coli O157:H7 str. TW14359 Escherichia coli O157:H7 str. TW14359 8206968 254667544 ECSP_6096 YP_003082230.1 putative exoprotein-precursor Virulence factor MUTATION: espP mutant is attenuated in calves [Ref6676:Dziva et al., 2007]. COG4625S, under S: Function unknown 17451446 3792 traT from Escherichia coli Vir68 Escherichia coli Vir68 8164705 253801054 pVir_80 YP_003034055.1 conjugal transfer surface exclusion protein TraT Virulence factor MUTATION: traT mutants have altered membrane permeability and are sensitive to hydrophobic antibiotics [Ref6228:Sukupolvi and O'Connor, 1987]. 3323837 3793 hlyC from Escherichia coli Vir68 Escherichia coli Vir68 8164736 253801086 pVir_111 YP_003034087.1 hemolysin activating protein HlyC Virulence factor MUTATION: hlyC is required for the acylation and therefore activation of the toxin hemolysin which has the ability to lyse several different types of cells [Ref6656:Stanley et al., 1996]. 8793877 3794 ompA from Escherichia coli BW2952 Escherichia coli BW2952 7952946 238900215 BWG_0809 YP_002926011.1 outer membrane protein A (3a;II*;G;d) Virulence factor MUTATION: OmpA− strains are unable to cause actin condensation needed for invasion of brain epithelial cells [Ref6643:Prasadarao et al., 1999]. COG2885M, under M: Cell wall/membrane/envelope biogenesis 10531228 3795 dppA from Escherichia coli BW2952 Escherichia coli BW2952 7953267 238902633 BWG_3233 YP_002928429.1 dipeptide transporter Virulence factor MUTATION: dppA mutant was attenuated 10- to 50-fold compared to the wild type for colonization of the bladders and kidneys of mice [Ref6641:Haugen et al., 2007]. COG0747E, under E: Amino acid transport and metabolism 17074858 3796 rfaH from Escherichia coli BW2952 Escherichia coli BW2952 7953491 238902915 BWG_3518 YP_002928711.1 DNA-binding transcriptional antiterminator Virulence factor MUTATION: attenuated in mice [Ref6640:Nagy et al., 2002]. COG0250K, under K: Transcription 12117951 3797 fimH from Escherichia coli BW2952 Escherichia coli BW2952 7954265 238903413 BWG_4018 YP_002929209.1 minor component of type 1 fimbriae Virulence factor MUTATION: attenuated in mice and humans [Ref6644:Wiles et al., 2008]. 18482721 3798 tonB from Escherichia coli BW2952 Escherichia coli BW2952 7954839 238900485 BWG_1081 YP_002926281.1 membrane spanning protein in TonB-ExbB-ExbD complex Virulence factor MUTATION: attenuated in mice [Ref6234:Torres et al., 2001]. COG0810M, under M: Cell wall/membrane/envelope biogenesis 11553558 3799 fliC from Escherichia coli BW2952 Escherichia coli BW2952 7955988 238901132 BWG_1732 YP_002926928.1 flagellar filament structural protein (flagellin) Virulence factor MUTATION: fliC deletion mutants had reduced adherence in bovine tissue compared to the wild type EHEC and EPEC strains [Ref6642:Erdem et al., 2007]. COG1344N, under N: Cell motility 17693516 3800 dsbA from Escherichia coli BW2952 Escherichia coli BW2952 7956141 238902928 BWG_3531 YP_002928724.1 periplasmic protein disulfide isomerase I Virulence factor MUTATION: mutation of dsbA led to degradation of bundilin and an absence of localized adherence to host cells in EPEC [Ref6645:Donnenberg et al., 1997]. COG1651O, under O: Posttranslational modification, protein turnover, chaperones 9224871 4876 K1 Capsule Escherichia coli Virulence factor MUTATION: K1 mutants were unable to cause meningitis in rats compared to wild type E. coli with a K1 capsule [Ref7302:Kim et al., 1992]. 1326000 4878 O75 O antigen Escherichia coli Virulence factor MUTATION: O75 antigen mutant were were more sensitive to killing by both polymorphonuclear leukocytes (PMNs) and monocytes than the wild type [Ref7304:Burns and Hull, 1999]. 10417134 4879 K5 capsular antigen Escherichia coli Virulence factor MUTATION: K5 deficient mutant is more sensitive to killing by polymorphonuclear leukocytes (PMNs) and monocytes than the wild type [Ref7304:Burns and Hull, 1999]. 10417134 4909 carA Escherichia coli str. K-12 substr. MG1655 949025 16128026 b0032 U00096 NP_414573 carbamoyl phosphate synthetase small subunit, glutamine amidotransferase Virulence factor MUTATION: A carAB mutant is attenuated in turkeys [Ref7338:Kwaga et al., 1994]. COG0505EF, under E: Amino acid transport and metabolism; F: Nucleotide transport and metabolism 8063392 4910 carB Escherichia coli str. K-12 substr. MG1655 944775 16128027 b0033 U00096 NP_414574 carbamoyl-phosphate synthase large subunit Virulence factor MUTATION: A carAB mutant is attenuated in turkeys [Ref7338:Kwaga et al., 1994]. COG0458EF, under E: Amino acid transport and metabolism; F: Nucleotide transport and metabolism 8063392