Protegen ID	Gene Name	Sequence Strain (Species/Organism)	NCBI Gene ID	NCBI Nucleotide GI	NCBI Protein GI	Locus Tag	Genbank Accession	Protein Accession	Protein Name	Protein Annotation	Molecule Role	Molecule Role Annotation	COG	PMID
5131	18K			557798815	557798816			AHA36956.1	18K immediate early protein		Virulence factor	 By successive rounds of selection for puromycin resistance and GFP expression, we have successfully constructed three recombinant viruses. In one, a "knock-in" mutant was created by inserting the Puro-EGFP cassette into a noncoding region of the FV3 genome (FV3-Puro/GFP). In the remaining two, KO mutants were constructed by replacement of the truncated viral homolog of eIF-2α (FV3-ΔvIF-2α) or the 18K IE gene (FV3-Δ18K) with the Puro-EGFP cassette  Viral replication of each recombinant in cell culture was similar to that of parental FV3; however, infection in Xenopus laevis tadpoles revealed that FV3-ΔvIF-2α and FV3-Δ18K replicated less and resulted in lower mortality than did GFP-FV3 and wild-type FV3. Our results suggest that 18K, which is conserved in all ranaviruses, and the truncated vIF-2α gene contribute to virulence. [Ref7528:Chen et al., 2011]		21865381