Protegen ID	Gene Name	Sequence Strain (Species/Organism)	NCBI Gene ID	NCBI Nucleotide GI	NCBI Protein GI	Locus Tag	Genbank Accession	Protein Accession	Protein Name	Protein Annotation	Molecule Role	Molecule Role Annotation	COG	PMID
5065	NL-S			1772821	1772822			AAB40136.1	virulence-associated protein		Virulence factor	the null mutant, E70/43, exhibited a marked reduction in pig virulence. In contrast to revertant or parental E70 where mortality was 100%, all E70/43-infected animals survived infection.; [Ref7468:Zsak et al., 1996]  		8971015
5066	UK			2905984	2905985			AAC03759.1	virulence determinant		Virulence factor	Although deletion of UK did not affect the growth characteristics of the virus in macrophage cell cultures, deltaUK exhibited reduced virulence in infected pigs. ; [Ref7469:Zsak et al., 1998]		9444996
5067	9GL			6759648	6759649			AAF27977.1	Za-9GL protein		Virulence factor	The Delta9GL virus was markedly attenuated in swine. In contrast to 9GL-R infection, where mortality was 100%, all Delta9GL-infected animals survived infection. With the exception of a transient fever response in some animals, Delta9GL-infected animals remained clinically normal and exhibited significant 100- to 10,000-fold reductions in viremia titers. All pigs previously infected with Delta9GL survived infection when subsequently challenged with a lethal dose of virulent parental MAL. Thus, ASFV 9GL gene deletion mutants may prove useful as live-attenuated ASF vaccines.  [Ref7470:Lewis et al., 2000]		10627538
5068	MGF530			433103	433104			AAA50527.1	multigene family 530 protein		Virulence factor	both MS16 and BA71V had significant deletions in the region containing members of multigene family 360 (MGF 360) and MGF530. Deletion of this same region from highly pathogenic ASFV isolate Pr4 significantly reduced viral growth in macrophage cell cultures. Given that macrophages are the primary target cells of ASFV in swine, it is likely that these genes are also of significance for viral pathogenesis and virulence in domestic swine. Future studies will examine this possibility.  [Ref7471:Zsak et al., 2001]		11238833
5069	MGF360				210601							both MS16 and BA71V had significant deletions in the region containing members of multigene family 360 (MGF 360) and MGF530. Deletion of this same region from highly pathogenic ASFV isolate Pr4 significantly reduced viral growth in macrophage cell cultures. Given that macrophages are the primary target cells of ASFV in swine, it is likely that these genes are also of significance for viral pathogenesis and virulence in domestic swine. Future studies will examine this possibility .  [Ref7471:Zsak et al., 2001]		11238833
5075	K196R	African swine fever virus	1488815		9628158	ASFVgp045	AF504896	NP_042744	thymidine kinase		Virulence factor	The Malawi TK deletion mutant showed reduced virulence for swine, producing transient fevers, lower viremia titers, and reduced mortality. In contrast, 100% mortality was observed for swine inoculated with the TK+ revertant virus.  [Ref7477:Moore et al., 1998]		9811782