Protegen ID	Gene Name	Sequence Strain (Species/Organism)	NCBI Gene ID	NCBI Nucleotide GI	NCBI Protein GI	Locus Tag	Genbank Accession	Protein Accession	Protein Name	Protein Annotation	Molecule Role	Molecule Role Annotation	COG	PMID
4461	TS	Trypanosoma cruzi strain CL Brener	3533424		71402314	Tc00.1047053504593.1	AAHK01002618	XP_804086			Virulence factor	MUTATION: T. cruzi isolates that express and shed higher amounts of trans-sialidase are more virulent in mice [Ref6999:Risso et al., 2004].; 		15181573
4462	Tc52				32395938				thiol transferase Tc52		Virulence factor	MUTATION: Tc52 mutant is attenuated in mice [Ref7000:Garzón et al., 2003].; 		12946866
4463	LYT1 				343407444				LYT1		Virulence factor	the deletion of both LYT1 alleles in the CL Brenner strain (the wild type [WT]) resulted in a null mutant T. cruzi clone (L16) that shows an attenuated phenotype in cell culture models. The infection of adult Swiss mice with 10(3) trypomastigotes of both clones revealed a significant reduction in infective behavior of L16, as shown by direct parasitemia, spleen index, and quantitation of tissue parasite burden, suggesting the loss of virulence in the null mutant clone.[Ref7001:Zago et al., 2008]; 		17938222
5132	 PLA1 													
5152	P85.1													
5153	p85.1	Symbiobacterium thermophilum IAM 14863	2981057		51893741	STH2603	AP006840	YP_076432	hypothetical protein		Virulence factor	p85.1 is expressed as mRNA and the corresponding protein is found on the surface of virulent Trypanosoma cruzi but not attenuated ones. virulent parasites not only gained the ability to cause high parasitemia but also caused higher mortality rates than the attenuated parasites.		
5176	DHFR-TS				1899001				dihydrofolate reductase-thymidylate synthase		Virulence factor	The dhfr-ts(+/-) mutant is possible to generate genetically attenuated T. cruzi parasites able to confer protection against further T. cruzi infections.; [Ref7576:Perez et al., 2011][Ref7577:Pérez and Basombrío, 2012]		22180798, 22705838
5200	Tc-CRT 	Trypanosoma cruzi strain CL Brener	3543786		71423784		AAHK01000598	XP_812571			Virulence factor	deletion of one copy of the TcCRT gene in the attenuated TCC strain generated a safe and irreversibly gene-deleted live attenuated parasite with high immunoprotective properties. Our results also contribute to endorse the important role of TcCRT as a T. cruzi virulence factor.;    [Ref7614:Sánchez-Valdéz et al., 2014]		24551259
5219	PLA1				372001299				phospholipase A1		Virulence factor	T. cruzi PLA1 as a novel virulence factor implicated in parasite invasion.; [Ref7613:Belaunzarán et al., 2013]		23275096
5221	trans-sialidase 	Trypanosoma cruzi strain CL Brener	3537653		71410253		AAHK01001396	XP_807431			Virulence factor	The trans-sialidase (TS) is a virulence factor involved in cell invasion and pathogenesis that is differentially expressed in aggressive and less virulent parasite stocks.; [Ref7617:Burgos et al., 2013]		23536842
5224	PkNBPXa													
5225	PkNBPXa													
5226	PkNBPXa