PHIDIAS: Pathogen-Host Interaction Data Integration and Analysis System

Phinet Name: Shigella spp.
Phinet Information
Pathogen Name: Shigella spp.
Pathogen NIAID Category: NIAID Category B
Bio-objects
Bio-object 1: Actin filaments depolymerization. Lamellipodial formation
GI Number: 145247787 Accession Number: Type: Location: Description:
Bio-object 2: Endocytosis. Shiga Toxin (Subunit B) - membrane interaction
GI Number: 143770754 Accession Number: Type: Location: Function: Description:
Bio-object 3: Endoplasmic Reticulum. Shiga Toxin-Release of fragment A1(enzymatically active) from fragment A2
GI Number: 16120353 Accession Number: Type: Location: Function: Description:
Bio-object 4: Escape of Shigella from the vacuole into the cytoplasm
GI Number: 145259569 Accession Number: Type: Location: Description:
Bio-object 5: Expression of IcsA protein on Shigella surface
GI Number: 13448939 Accession Number: Type: Location: Description:
Bio-object 6: Furin
GI Number: 20336193 Accession Number: Type: Location: Function: Description:
Bio-object 7: Golgi Apparatus. Shiga Toxin-Subunit A nicking by protease Furin
GI Number: 145250049 Accession Number: Type: Location: Function: Description:
Bio-object 8: Inhibition of binding of aminoacyl - tRNA
GI Number: 30061571 Accession Number: Type: Location: Function: Description:
Bio-object 9: IpaA translocation via TTSS-Mxi-Ipa structure
GI Number: 31983523 Accession Number: Type: Location: Description:
Bio-object 10: IpaA-Vinculin complex formation
GI Number: 2642477 Accession Number: DB Links: Type: Location: Description:
Bio-object 11: IpaB and IpaC disrupt the vacuolar membrane
Type: Location: Description:
Bio-object 12: IpaB, IpaC, IpaD translocation via TTSS-MxiH-MxiI (TTSS-Mxi) complex
Type: Location: Function: Description:
Bio-object 13: IpaB-IpaC complex formation
Type: Location: Function: Description:
Bio-object 14: IpaB-IpaD complex. Control proteins flux
Type: Location: Function: Description:
Bio-object 15: IpaC modulates Cdc 42-dependent Filopodial formation
Type: Location: Description:
Bio-object 16: IpaC modulates Rac1-dependent Lamellipodial formation
Type: Location: Description:
Bio-object 17: Movement within the cytoplasm is mediated by IcsA
Type: Location: Description:
Bio-object 18: MxiH, MxiI secretion via TTSS. MxiH-MxiI structure formation
Type: Location: Function: Description:
Bio-object 19: Protein synthesis blocking and death of intoxicated cells
Type: Location: Description:
Bio-object 20: Ribosome. The A1 fragment cleaves the N-glycosidic bond in 28S rRNA
Type: Location: Function: Description:
Bio-object 21: Shiga Toxin realease ( Subunit A - Subunit B (pentameric ring))
Type: Location: Function: Description:
Bio-object 22: Shiga Toxin secretion by bacterial cells
Type: Location: Function: Description:
Bio-object 23: Shigella induction of apoptosis
Type: Location: Description:
Bio-object 24: Shigella internalization in vacuole
Type: Location: Description:
Bio-object 25: Shigella intracellular and intercellular spread
Type: Location: Description:
Bio-object 26: Shigella-induced cytoskeletal rearrangements
Type: Location: Description:
Bio-object 27: Subunit B - Receptor Gb3 interaction on host cell surface (Gb3 - Globotiaosyl ceramide)
Type: Location: Function: Description:
Bio-object 28: Trans-Golgi Network. Shiga Toxin-Subunit A nicking by protease Furin
Type: Location: Function: Description:
Bio-object 29: TTSS-MxiH(n), TTSS-MxiI(n). Needle like structure formation
Type: Location: Function: Description:
Bio-object 30: TTSS-MxiH-MxiI-IpaB-IpaD (TTSS-Mxi-Ipa) structure formation
Type: Location: Function: Description:
Bio-object 31: Type III Secretion System
Type: Location: Function: Description:
Bio-object 32: Vesicles containing Shiga Toxin. Shiga Toxin transport to the Trans-Golgi Network
Type: Location: Function: Description:
Interactions
Interaction 1: Interaction1
Input Objects: Shiga Toxin secretion by bacterial cells Output Objects: Shiga Toxin realease ( Subunit A - Subunit B (pentameric ring)) GO Evidence Code: Inferred from Direct Assay Description:
Interaction 2: Interaction2
Input Objects: Shiga Toxin realease ( Subunit A - Subunit B (pentameric ring)) Output Objects: Subunit B - Receptor Gb3 interaction on host cell surface (Gb3 - Globotiaosyl ceramide) GO Evidence Code: Inferred from Direct Assay Description:
Interaction 3: Interaction3
Input Objects: Subunit B - Receptor Gb3 interaction on host cell surface (Gb3 - Globotiaosyl ceramide) Output Objects: Endocytosis. Shiga Toxin (Subunit B) - membrane interaction GO Evidence Code: Inferred from Direct Assay Description:
Interaction 4: Interaction4
Input Objects: Endocytosis. Shiga Toxin (Subunit B) - membrane interaction Output Objects: Vesicles containing Shiga Toxin. Shiga Toxin transport to the Trans-Golgi Network GO Evidence Code: Inferred from Direct Assay Description:
Interaction 5: Interaction5
Cofactors: Furin Input Objects: Vesicles containing Shiga Toxin. Shiga Toxin transport to the Trans-Golgi Network Output Objects: Trans-Golgi Network. Shiga Toxin-Subunit A nicking by protease Furin GO Evidence Code: Inferred from Direct Assay Description:
Interaction 6: Interaction6
Cofactors: Furin Input Objects: Trans-Golgi Network. Shiga Toxin-Subunit A nicking by protease Furin Output Objects: Golgi Apparatus. Shiga Toxin-Subunit A nicking by protease Furin GO Evidence Code: Inferred from Direct Assay Description:
Interaction 7: Interaction7
Input Objects: Golgi Apparatus. Shiga Toxin-Subunit A nicking by protease Furin Output Objects: Endoplasmic Reticulum. Shiga Toxin-Release of fragment A1(enzymatically active) from fragment A2 GO Evidence Code: Inferred from Direct Assay Description:
Interaction 8: Interaction8
Input Objects: Endoplasmic Reticulum. Shiga Toxin-Release of fragment A1(enzymatically active) from fragment A2 Output Objects: Ribosome. The A1 fragment cleaves the N-glycosidic bond in 28S rRNA GO Evidence Code: Inferred from Direct Assay Description:
Interaction 9: Interaction9
Input Objects: Ribosome. The A1 fragment cleaves the N-glycosidic bond in 28S rRNA Output Objects: Inhibition of binding of aminoacyl - tRNA GO Evidence Code: Inferred from Direct Assay Description:
Interaction 10: Interaction10
Input Objects: Inhibition of binding of aminoacyl - tRNA Output Objects: Protein synthesis blocking and death of intoxicated cells GO Evidence Code: Inferred from Direct Assay Description:
Interaction 11: Interaction11
Input Objects: Type III Secretion System Output Objects: MxiH, MxiI secretion via TTSS. MxiH-MxiI structure formation GO Evidence Code: Inferred from Direct Assay Description:
Interaction 12: Interaction12
Input Objects: MxiH, MxiI secretion via TTSS. MxiH-MxiI structure formation Output Objects: TTSS-MxiH(n), TTSS-MxiI(n). Needle like structure formation GO Evidence Code: Inferred from Direct Assay Description:
Interaction 13: Interaction13
Input Objects: TTSS-MxiH(n), TTSS-MxiI(n). Needle like structure formation Output Objects: IpaB, IpaC, IpaD translocation via TTSS-MxiH-MxiI (TTSS-Mxi) complex GO Evidence Code: Inferred from Direct Assay Description:
Interaction 14: Interaction14
Input Objects: IpaB, IpaC, IpaD translocation via TTSS-MxiH-MxiI (TTSS-Mxi) complex Output Objects: TTSS-MxiH-MxiI-IpaB-IpaD (TTSS-Mxi-Ipa) structure formation GO Evidence Code: Inferred from Direct Assay Description:
Interaction 15: Interaction15
Input Objects: TTSS-MxiH-MxiI-IpaB-IpaD (TTSS-Mxi-Ipa) structure formation Output Objects: IpaB-IpaD complex. Control proteins flux, IpaB-IpaC complex formation GO Evidence Code: Inferred from Direct Assay Description:
Interaction 16: Interaction16
Input Objects: IpaB-IpaC complex formation Output Objects: IpaC modulates Cdc 42-dependent Filopodial formation, IpaC modulates Rac1-dependent Lamellipodial formation, IpaA translocation via TTSS-Mxi-Ipa structure GO Evidence Code: Inferred from Direct Assay Description:
Interaction 17: Interaction17
Input Objects: IpaA translocation via TTSS-Mxi-Ipa structure Output Objects: IpaA-Vinculin complex formation GO Evidence Code: Inferred from Direct Assay Description:
Interaction 18: Interaction18
Input Objects: IpaA-Vinculin complex formation Output Objects: Actin filaments depolymerization. Lamellipodial formation GO Evidence Code: Inferred from Direct Assay Description:
Interaction 19: Interaction19
Input Objects: Actin filaments depolymerization. Lamellipodial formation Output Objects: Shigella-induced cytoskeletal rearrangements GO Evidence Code: Inferred from Direct Assay Description:
Interaction 20: Interaction20
Input Objects: Shigella-induced cytoskeletal rearrangements Output Objects: Shigella internalization in vacuole GO Evidence Code: Inferred from Direct Assay Description:
Interaction 21: Interaction21
Input Objects: Shigella internalization in vacuole Output Objects: IpaB and IpaC disrupt the vacuolar membrane GO Evidence Code: Inferred from Direct Assay Description:
Interaction 22: Interaction22
Input Objects: IpaB and IpaC disrupt the vacuolar membrane Output Objects: Escape of Shigella from the vacuole into the cytoplasm GO Evidence Code: Inferred from Direct Assay Description:
Interaction 23: Interaction23
Input Objects: Escape of Shigella from the vacuole into the cytoplasm Output Objects: Expression of IcsA protein on Shigella surface GO Evidence Code: Inferred from Direct Assay Description:
Interaction 24: Interaction24
Input Objects: Expression of IcsA protein on Shigella surface Output Objects: Movement within the cytoplasm is mediated by IcsA GO Evidence Code: Inferred from Direct Assay Description:
Interaction 25: Interaction25
Input Objects: Movement within the cytoplasm is mediated by IcsA Output Objects: Shigella intracellular and intercellular spread GO Evidence Code: Inferred from Direct Assay Description:
Interaction 26: Interaction26
Input Objects: Shigella intracellular and intercellular spread Output Objects: Shigella induction of apoptosis GO Evidence Code: Inferred from Direct Assay Description:
Interaction 27: Interaction27
Input Objects: IpaC modulates Cdc 42-dependent Filopodial formation, IpaC modulates Rac1-dependent Lamellipodial formation Output Objects: Shigella-induced cytoskeletal rearrangements GO Evidence Code: Inferred from Direct Assay Description:
Pathways

Phinet Name: Shigella spp.